Results 341 to 350 of about 1,220,561 (394)

Hijacking the Host Clock: A Nematode Effector Antagonizes Soybean Circadian Defense and Translation Control

Advanced Science, EarlyView.
Soybean employs its circadian clock, governed by GmCCA1, to rhythmically defend against soybean cyst nematodes. The pathogen retaliates by secreting the effector Hg4E02, which hijacks the clock to suppress defense and co‐opt the host's translation machinery for nutrient acquisition.
Xingwei Wang, Yufeng Xu, Yanfei Hu, Lijun Cao, Ru Jiang, Changtian Chen, Rick Masonbrink, Thomas Maier, Yuchen Tu, Yabo Shi, Enhui Liu, Lingan Kong, Chan Guo, Wei Zhao, Peng Shi, Wenzhen Du, Andrew Severin, Thomas Baum, Deliang Peng, Huan Peng, Mian Zhou, Wei Wang   +21 more
wiley   +1 more source

Genetic Characterization of Palyam Serogroup Viruses Isolated in Japan from 1984 to 2018 and Development of a Real-Time RT-PCR Assay for Broad Detection of Palyam Serogroup Viruses and Specific Detection of Chuzan (Kasba) and D'Aguilar Viruses. [PDF]

Pathogens
Shirafuji H, Kishida N, Murota K, Suda Y, Yanase T.   +4 more
europepmc   +1 more source

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Stepwise Optimization of Real-Time RT-PCR Analysis.

Methods in molecular biology, 2023
Quantitative real-time reverse transcription PCR (qRT-PCR) analysis has been used routinely to quantify gene expression levels. Primer design and the optimization of qRT-PCR parameters are critical for the accuracy and reproducibility of qRT-PCR analysis.
N. Maren, James R. Duduit, Debao Huang, Fang Zhao, T. Ranney, Wusheng Liu   +5 more
semanticscholar   +3 more sources

Quantification of mRNA using real-time RT-PCR

Nature Protocols, 2006
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA.
T. Nolan, R. Hands, S. Bustin
semanticscholar   +3 more sources

Real-time RT-PCR for norovirus screening in shellfish.

Journal of Virological Methods, 2005
Real-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II.
Fabienne Loisy, R. Atmar, Patrice Guillon, P. L. Cann, M. Pommepuy, F. L. Guyader   +5 more
semanticscholar   +4 more sources

Real-Time RT-PCR: cDNA Synthesis

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol uses the Superscript II First-Strand Synthesis System for the generation of cDNA from total RNA.
Wolfgang, Kusser, Sandrine, Javorschi, Martin A, Gleeson   +2 more
openaire   +2 more sources

Quantitative real-time RT-PCR assay for PCA3

Clinical Biochemistry, 2008
To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort.The RT-PCR assay for PCA3 is based on target-specific lanthanide probes ...
Riina-Minna, Väänänen, Maria, Rissanen, Otto, Kauko, Siina, Junnila, Ville, Väisänen, Jussi, Nurmi, Kalle, Alanen, Martti, Nurmi, Kim, Pettersson   +8 more
openaire   +2 more sources

Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus

Molecular and Cellular Probes, 2017
Deping Song, Yu Ye
exaly   +2 more sources

Identification of nasal blood by real-time RT-PCR

Legal Medicine, 2012
A new approach for the identification of body fluid stains by comparing specific mRNA expression levels has been extensively studied in recent years. Here, we examine whether nasal blood, which is regarded as one of the most difficult types of blood to identify, can be identified by comparing mRNA expression levels of target genes specific to saliva ...
Koichi, Sakurada, Tomoko, Akutsu, Ken, Watanabe, Mineo, Yoshino   +3 more
openaire   +2 more sources