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The evolution of real-time PCR machines to real-time PCR chips
Biosensors and Bioelectronics, 2010Development of Micro-Electro-Mechanical Systems (MEMS) technology has recently allowed the migration of real-time polymerase chain reaction (PCR) machines to lab-on-a-chip systems. The miniaturization of biological instruments has been studied extensively, with several prototypes constructed and tested.
G B Lee, Pei-Jer Chen, Dasheng Lee
exaly +3 more sources
Real-time PCR detection chemistry
Clinica Chimica Acta, 2015Real-time PCR is the method of choice in many laboratories for diagnostic and food applications. This technology merges the polymerase chain reaction chemistry with the use of fluorescent reporter molecules in order to monitor the production of amplification products during each cycle of the PCR reaction.
E, Navarro +3 more
exaly +3 more sources
Enfermedades infecciosas y microbiologia clinica, 2004
The development of instruments that allow real-time monitoring of fluorescence within PCR reaction vessels is a significant advance in clinical bacteriology. The technology is very flexible, and many alternative instruments and fluorescent probe systems are currently available.
Slade, Neda, Kapitanović, Sanja
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The development of instruments that allow real-time monitoring of fluorescence within PCR reaction vessels is a significant advance in clinical bacteriology. The technology is very flexible, and many alternative instruments and fluorescent probe systems are currently available.
Slade, Neda, Kapitanović, Sanja
+8 more sources
Forensic Science, Medicine, and Pathology, 2007
Research into the field of DNA has increased dramatically in the years since DNA profiling was first identified. Since then, techniques have been developed to identify individuals from DNA samples equivalent to a single cell. Development of these techniques meant that the ability to accurately quantitate the DNA in such samples was required to evolve ...
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Research into the field of DNA has increased dramatically in the years since DNA profiling was first identified. Since then, techniques have been developed to identify individuals from DNA samples equivalent to a single cell. Development of these techniques meant that the ability to accurately quantitate the DNA in such samples was required to evolve ...
+6 more sources
2015
Wie bereits unter Kap. 13 erwahnt, ist die Quantifizierung der amplifizierten PCR-Produkte nach 25–30 Zyklen nicht mehr sehr einfach. Damit eine Quantifizierung der zu untersuchenden Amplicons (ca. 50–150 bp Lange) ermoglicht werden kann, haben sich verschiedene optische PCR-Systeme zur „online“ Beobachtung des Amplifikationsstatus etabliert (Tab. 14.1)
Hans-Joachim Müller +1 more
openaire +2 more sources
Wie bereits unter Kap. 13 erwahnt, ist die Quantifizierung der amplifizierten PCR-Produkte nach 25–30 Zyklen nicht mehr sehr einfach. Damit eine Quantifizierung der zu untersuchenden Amplicons (ca. 50–150 bp Lange) ermoglicht werden kann, haben sich verschiedene optische PCR-Systeme zur „online“ Beobachtung des Amplifikationsstatus etabliert (Tab. 14.1)
Hans-Joachim Müller +1 more
openaire +2 more sources
Real-Time Multiplex PCR Assays
Methods, 2001The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra.
C T, Wittwer +3 more
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2009
Over the past few years there has been a considerable development of DNA amplification by polymerase chain reaction (PCR), and real-time PCR has now superseded conventional PCR techniques in many areas, e.g., the quantification of nucleic acids and genotyping.
A. Evrard, N. Boulle, G.s Lutfalla
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Over the past few years there has been a considerable development of DNA amplification by polymerase chain reaction (PCR), and real-time PCR has now superseded conventional PCR techniques in many areas, e.g., the quantification of nucleic acids and genotyping.
A. Evrard, N. Boulle, G.s Lutfalla
openaire +1 more source
Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic
2012Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical.
Barletta, Francesca +2 more
openaire +3 more sources