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Enfermedades infecciosas y microbiologia clinica, 2004
The development of instruments that allow real-time monitoring of fluorescence within PCR reaction vessels is a significant advance in clinical bacteriology. The technology is very flexible, and many alternative instruments and fluorescent probe systems are currently available.
Slade, Neda, Kapitanović, Sanja
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The development of instruments that allow real-time monitoring of fluorescence within PCR reaction vessels is a significant advance in clinical bacteriology. The technology is very flexible, and many alternative instruments and fluorescent probe systems are currently available.
Slade, Neda, Kapitanović, Sanja
+8 more sources
Forensic Science, Medicine, and Pathology, 2007
Research into the field of DNA has increased dramatically in the years since DNA profiling was first identified. Since then, techniques have been developed to identify individuals from DNA samples equivalent to a single cell. Development of these techniques meant that the ability to accurately quantitate the DNA in such samples was required to evolve ...
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Research into the field of DNA has increased dramatically in the years since DNA profiling was first identified. Since then, techniques have been developed to identify individuals from DNA samples equivalent to a single cell. Development of these techniques meant that the ability to accurately quantitate the DNA in such samples was required to evolve ...
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2010
Die vielfaltigen Anwendungsmoglichkeiten der Polymerasekettenreaktion (polymerase chain reaction, PCR) machen sie zu einer der wichtigsten und am haufigsten eingesetzten Methoden in der molekularbiologischen Forschung und Diagnostik. Fur diese Technologie wurde der Erfinder der Methode, Kary Mullis, 1993 mit dem Nobelpreis ausgezeichnet.
Regina Konrad, Ulrich Busch
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Die vielfaltigen Anwendungsmoglichkeiten der Polymerasekettenreaktion (polymerase chain reaction, PCR) machen sie zu einer der wichtigsten und am haufigsten eingesetzten Methoden in der molekularbiologischen Forschung und Diagnostik. Fur diese Technologie wurde der Erfinder der Methode, Kary Mullis, 1993 mit dem Nobelpreis ausgezeichnet.
Regina Konrad, Ulrich Busch
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2015
Wie bereits unter Kap. 13 erwahnt, ist die Quantifizierung der amplifizierten PCR-Produkte nach 25–30 Zyklen nicht mehr sehr einfach. Damit eine Quantifizierung der zu untersuchenden Amplicons (ca. 50–150 bp Lange) ermoglicht werden kann, haben sich verschiedene optische PCR-Systeme zur „online“ Beobachtung des Amplifikationsstatus etabliert (Tab. 14.1)
Hans-Joachim Müller +1 more
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Wie bereits unter Kap. 13 erwahnt, ist die Quantifizierung der amplifizierten PCR-Produkte nach 25–30 Zyklen nicht mehr sehr einfach. Damit eine Quantifizierung der zu untersuchenden Amplicons (ca. 50–150 bp Lange) ermoglicht werden kann, haben sich verschiedene optische PCR-Systeme zur „online“ Beobachtung des Amplifikationsstatus etabliert (Tab. 14.1)
Hans-Joachim Müller +1 more
openaire +2 more sources
Real-time PCR detection chemistry
Clinica Chimica Acta, 2015Real-time PCR is the method of choice in many laboratories for diagnostic and food applications. This technology merges the polymerase chain reaction chemistry with the use of fluorescent reporter molecules in order to monitor the production of amplification products during each cycle of the PCR reaction.
E, Navarro +3 more
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Real-Time Multiplex PCR Assays
Methods, 2001The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra.
C T, Wittwer +3 more
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