Results 141 to 150 of about 512,365 (262)

The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus [PDF]

, 2010
Hong Tian, Jingyan Wu, Youjun Shang, Yan Cheng, Xiangtao Liu   +4 more
openalex   +1 more source

Bioengineered Pancreatic Cancer Immunosuppressive Microenvironment Models for Screening Immunotherapies

Advanced Healthcare Materials, EarlyView.
Bioengineering tumor‐stroma pancreatic cancer models with potential to emulate the native immunosuppressive tumor microenvironment. Cancer‐on‐a‐bead models exhibited higher relevance for screening immunotherapies and modulating the protumoral PDAC microenvironment. ABSTRACT Pancreatic cancer is notably resistant to treatment, primarily due to its dense
Maria V. Monteiro, Margarida Henriques‐Pereira, Bruno M. Neves, Vítor M. Gaspar, João F. Mano   +4 more
wiley   +1 more source

Development of Reverse Transcription (RT)-PCR and Real-Time RT-PCR Assays for Rapid Detection and Quantification of Viable Yeasts and Molds Contaminating Yogurts and Pasteurized Food Products

, 2003
Gianluca Bleve, Lucia Rizzotti, Franco Dellaglio, Sandra Torriani   +3 more
openalex   +1 more source

A Heparin‐Functionalized Scaffold with HB‐EGF Immobilization for Tissue Engineering

Advanced Healthcare Materials, EarlyView.
In this study, a heparin‐functionalized scaffold is designed for bio‐mimicking growth factors reservoir function of extracellular matrix. Due to the presence of heparin‐binding domain, heparin‐binding epidermal growth factor‐like growth factor (HB‐EGF) is effectively and spatially captured by heparin‐functionalized scaffold.
Bowu Peng, Huajian Chen, Chengyu Lu, Tianjiao Zeng, Man Wang, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen   +8 more
wiley   +1 more source

Modification and Validation of a Reference Real-Time RT-PCR Method for the Detection of a New African Horse Sickness Virus Variant. [PDF]

Microorganisms
Morales J, Ruano MJ, Tena-Tomás C, van Schalkwyk A, Loundras EA, Valero-Lorenzo M, López-Herranz A, Romito M, Batten C, Villalba R, Agüero M.   +10 more
europepmc   +1 more source

Spherical Skin Model: Stratified Co‐Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening

Advanced Healthcare Materials, EarlyView.
Models of the human skin must combine the relevant biological contents and suitable biomaterials with the correct spatial organization. Performing compound screening on such in vitro models also requires fast and reproducible production methods of the models.
Elisa Lenzi, Nadia Vertti‐Quintero, Julien Husson, Anne‐Laure Bulteau, Carine Nizard, Karl Pays, Sébastien Sart, Charles N. Baroud   +7 more
wiley   +1 more source

Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR. [PDF]

PLoS One
Phimolsannousith V, Vongsouvath M, Kiedsathid P, Vongsouvath M, Ashley EA, Dubot-Pérès A.   +5 more
europepmc   +1 more source

Development of a Synthetic 3D Platform for Compartmentalized Kidney In Vitro Disease Modeling

Advanced Healthcare Materials, EarlyView.
A fully synthetic, compartmentalized 3D kidney disease model is introduced. The kidney model combines a PEG‐based hydrogel matrix with anisotropic, enzymatically degradable rod‐shaped microgels to spatially arrange a triple co‐culture of key renal epithelial, endothelial, and fibroblast cells.
Ninon Möhl, Daphne Bouwens, Johanna Abele, Aline Hans, Tanja Topic, Daniel Günther, Jitske Jansen, Rafael Kramann, Laura De Laporte   +8 more
wiley   +1 more source

Type-specific EV-D68 real-time RT-PCR assay for the detection of all extant enterovirus D68 strains. [PDF]

J Clin Microbiol
Ng TFF, Nix WA, Rogers SL, Emery B, Chern S-W, Butler K, Oberste MS.   +6 more
europepmc   +1 more source

Establishing a molecular toolbox of lineage-specific real-time RT-PCR assays for the characterization of foot-and-mouth disease viruses circulating in Asia. [PDF]

Front Vet Sci, 2023
Saduakassova MA, Wood BA, Henry E, Gray AR, Mioulet V, Sultanov AA, Wadsworth J, Knowles NJ, Di Nardo A, King DP, Bachanek-Bankowska K.   +10 more
europepmc   +1 more source