Results 121 to 130 of about 18,885,351 (305)

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Evaluación comparativa de RT-PCR punto final, RT-PCR en tiempo real y RT-LAMP en el diagnóstico del virus GLRaV-3 asociado al enrollamiento de la hoja en plantas de vid [PDF]

, 2021
El enrollamiento de la hoja de la vid (grapevine leafroll disease, GLD) es causada por un complejo de virus de la familia Closteroviridae; dentro de estos, el virus más representativo de la enfermedad es el GLRaV-3, perteneciente a la especie Grapevine ...
Odett Mariana Sandez Salas
core  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Real-time RT-PCR analysis of IPFP.

, 2016
The mRNA expression of Nampt, leptin, VEGF, TNF-α, IL-6 and TGF-β1in the IPFP was evaluated by real-time RT-PCR analysis in the indicated mice. Values are the means ± 1 SD of ten mice per group. NL, normal loading; ND, normal diet; HFD, high-fat diet; TS,
Yoshinori Asou (310747), Maierhaba Ailixiding (3112011), Hiroki Ochi (310745), Jinying Piao (310739), Zulipiya Aibibula (3112008), Munetaka Iwata (322242), Yasushi Hara (402209), Atsushi Okawa (310752), Guangwen Jin (3112014)   +8 more
core   +1 more source

Determinación de Salmonella spp. por PCR en tiempo real y método convencional en canales de bovinos y en alimentos de la vía pública de Montería, Córdoba Detection of Salmonella spp. by real time PCR and standard methods in cattle carcasses and public fast food outlets in Montería, Córdoba

Infectio, 2008
Objetivo. Detectar Salmonella spp. por PCR en tiempo real (PCR-TR) y el método convencional en alimentos de la vía pública y canales de bovino de una planta certificada de beneficio animal con sistema HACCP de Montería. Materiales y métodos.
Edna Yánez, Salim Máttar, Alba Durango
doaj  

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis

The Scientific World Journal, 2014
The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A ...
K. T. Sultankulova, O. V. Chervyakova, N. S. Kozhabergenov, K. A. Shorayeva, V. M. Strochkov, M. B. Orynbayev, N. T. Sandybayev, A. R. Sansyzbay, A. V. Vasin   +8 more
doaj   +1 more source

Antemortem Diagnosis of Rabies From Skin: Comparison of nested RT-PCR with TaqMan real time PCR

Brazilian Journal of Veterinary Pathology, 2012
Molecular techniques were adopted to diagnose rabies viral RNA from skin biopsy samples collected from 20 animals. Nested RT-PCR and TaqMan real time PCR techniques have successfully diagnosed rabies viral RNA in 9 and 11 skin samples, respectively.
Karan Bansal, Charan Kamal K Singh, Bhupinder Singh Sandhu, Deepak Deka, Mangesh Dandale, Naresh Kumar Sood   +5 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Real-Time RT-PCR Detection of Equine Coronavirus

, 2016
Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and ...
Miszczak, Fabien, Kin, Nathalie, Tesson, Vincent, Vabret, Astrid   +3 more
openaire   +1 more source