PLoS ONE, 2021 At the start of the COVID-19 pandemic, the Centers for Disease Control and Prevention (CDC) designed, manufactured, and distributed the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel for SARS-CoV-2 detection.
Justin S. Lee +24 more
openalex +3 more sources
Comparative Performance of Digital PCR and Real-Time RT-PCR in Respiratory Virus Diagnostics [PDF]
VirusesBackground: Respiratory viral infections pose a major global health burden, and molecular diagnostics such as Real-Time RT-PCR have revealed frequent co-infections. However, precise quantification of viral RNA remains challenging.
Irene Bianconi +5 more
doaj +2 more sources
Enhanced rabies surveillance in roadkill specimens by real-time RT-PCR. [PDF]
PLoS Neglected Tropical DiseasesRoadkill specimens are an important source of samples for enhanced rabies surveillance (ERS) in areas where other methods of sample collection may not be practical.
Crystal M Gigante +8 more
doaj +2 more sources
Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
Emerging Infectious Diseases, 2020 Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance ...
Jesse J. Waggoner +10 more
doaj +2 more sources
Application of Metagenomic Next-Generation Sequencing (mNGS) and real-time RT-PCR Using different nasopharyngeal swabs in Diagnosing SARS-CoV-2 Omicron [PDF]
, 2023 LI S, Zhang Y, Tong p, Yang w.
europepmc +2 more sources
Application of standard and molecular methods for the diagnosis of newcastle disease [PDF]
Archives of Biological Sciences, 2012 Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007 ...
Vidanović D. +6 more
doaj +1 more source
Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay [PDF]
Veterinary World, 2013 Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV). Materials and Methods: The BTV serotype 21 isolate (KMNO7) was isolated from Andhra Pradesh and propagated ...
Gaya Prasad +5 more
doaj +1 more source
Viral Dynamics and Real-Time RT-PCR Ct Values Correlation with Disease Severity in COVID-19
Diagnostics, 2021 Real-time RT-PCR is considered the gold standard confirmatory test for coronavirus disease 2019 (COVID-19). However, many scientists disagree, and it is essential to understand that several factors and variables can cause a false-negative test.
A. Rabaan +15 more
semanticscholar +1 more source
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2020 Background The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially ...
V. Corman +23 more
semanticscholar +1 more source
استفاده از Real time RT-PCR جهت شناسایی ویروس زبان آبی ایران [PDF]
Archives of Razi Institute, 2011 طی سال های 1388-1389 از روش real time RT-PCR و RT-PCRمعمولی جهت شناسایی ویروس زبان آبی در 310 گوسفند مشکوک به بیماری زبان آبی استفاده شد. قطعاتS1 (ژن پلی مراز) و S10 (ژن NS3 ) بعنوان ژنهای حراست شده در گروه سرمی زبان آبی به ترتیب توسط rRT-PCRوRT-PCR ...
عبدالحمید شوشتری +3 more
doaj +1 more source