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Comparative Performance of Digital PCR and Real-Time RT-PCR in Respiratory Virus Diagnostics [PDF]

Viruses
Background: Respiratory viral infections pose a major global health burden, and molecular diagnostics such as Real-Time RT-PCR have revealed frequent co-infections. However, precise quantification of viral RNA remains challenging.
Irene Bianconi, Giovanna Viviana Pellecchia, Elisabetta Maria Incrocci, Fabio Vittadello, Maira Nicoletti, Elisabetta Pagani   +5 more
doaj   +2 more sources

Enhanced rabies surveillance in roadkill specimens by real-time RT-PCR. [PDF]

PLoS Neglected Tropical Diseases
Roadkill specimens are an important source of samples for enhanced rabies surveillance (ERS) in areas where other methods of sample collection may not be practical.
Crystal M Gigante, Claire Hartloge, Rene Edgar Condori, Jordona D Kirby, Lauren Hovis, Kathleen M Nelson, Ryan Wallace, Yu Li, Richard B Chipman   +8 more
doaj   +2 more sources

Application of standard and molecular methods for the diagnosis of newcastle disease [PDF]

Archives of Biological Sciences, 2012
Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007 ...
Vidanović D., Šekler M., Polaček V., Vasković N., Ašanin Ružica, Milić N., Nišavić J.   +6 more
doaj   +1 more source

Quantitative real-time RT-PCR – a perspective [PDF]

Journal of Molecular Endocrinology, 2005
The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect ...
S A, Bustin, V, Benes, T, Nolan, M W, Pfaffl   +3 more
openaire   +2 more sources

Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay [PDF]

Veterinary World, 2013
Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV). Materials and Methods: The BTV serotype 21 isolate (KMNO7) was isolated from Andhra Pradesh and propagated ...
Gaya Prasad, Pawan Kumar, Supriya A, P Minakshi, Ranjan Koushlesh, T.M. Vishwaradhya   +5 more
doaj   +1 more source

استفاده از Real time RT-PCR جهت شناسایی ویروس زبان آبی ایران [PDF]

Archives of Razi Institute, 2011
طی سال های 1388-1389 از روش real time RT-PCR و RT-PCRمعمولی جهت شناسایی ویروس زبان آبی در 310 گوسفند مشکوک به بیماری زبان آبی استفاده شد. قطعاتS1 (ژن پلی مراز) و S10 (ژن NS3 ) بعنوان ژنهای حراست شده در گروه سرمی زبان آبی به ترتیب توسط rRT-PCRوRT-PCR ...
عبدالحمید شوشتری, ف. جیرانی, همایون مهروانی, س.م. عظیمی   +3 more
doaj   +1 more source

تشخیص سریع و دقیق ویروس تب برفکی با روش PCR Real time در نمونه‌های بالینی [PDF]

Archives of Razi Institute, 2012
طی سالهای 1390-1389، از روش PCR Real-time برای ردیابی RNA ویروس تب برفکی در 147 نمونه اپیتلیوم از دام‌های مشکوک به بیماری تب برفکی که از کانون‌های مختلف اخذ گردیده بود استفاده شد.
همایون مهروانی, م. عظیمی, ا. احمدی, ف. جیرانی   +3 more
doaj   +1 more source

Real-time RT-PCR diagnostics of virus causing COVID-19

Фармакоэкономика, 2020
Aim: the study was aimed to develop a reagent kit for the real-time RT-PCR diagnostics of virus causing COVID-19.Materials and Methods. Three target sites were chosen in the genome SARS-CoV-2.
E. V. Goncharova, A. E. Donnikov, V. V. Kadochnikova, S. A. Morozova, M. N. Boldyreva, I. S. Galkina, D. V. Blinov   +6 more
doaj   +1 more source

Real-Time RT-PCR for the Detection of Lyssavirus Species [PDF]

Journal of Veterinary Medicine, 2014
The causative agents of rabies are single-stranded, negative-sense RNA viruses in the genus Lyssavirus of Rhabdoviridae, consisting of twelve classified and three as yet unclassified species including classical rabies virus (RABV). Highly neurotropic RABV causes rapidly progressive encephalomyelitis with nearly invariable fatal outcome.
Deubelbeiss, A., Zahno, M.-L., Zanoni, M., Bruegger, D., Zanoni, R.   +4 more
openaire   +2 more sources

Development of an RNA Extraction Protocol for Norovirus from Raw Oysters and Detection by qRT-PCR and Droplet-Digital RT-PCR

Foods, 2021
Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR ...
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irène Iugovaz, Neda Nasheri   +4 more
doaj   +1 more source