Results 31 to 40 of about 18,885,351 (305)
The KUP family of potassium transporters in plants is large but poorly characterized. We isolated and characterized the first KUP transporter from a legume, LjKUP of Lotus japonicus.
Guilhem Desbrosses +3 more
doaj +1 more source
Introduction: Acute Chagas disease involving reactivation can occur after organ transplant, and follow-up by direct parasitological or molecular methods is essential for monitoring the parasitic load in such patients. In contrast, there is a little data
Juliana Jesus Guimarães Ferreira +9 more
doaj +1 more source
Detection of Tumor Cell-Specific mRNA in the Peripheral Blood of Patients with Breast Cancer-Evaluation of Several Markers with Real-Time Reverse Transcription-PCR [PDF]
It is widely known that cells from epithelial tumors, e. g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic ...
Simone Hofmann +20 more
core +1 more source
Real-time RT-PCR for the detection and quantitation of Oropouche virus [PDF]
Oropouche virus (OROV) causes an acute, systemic febrile illness, and in certain regions of South America, this represents the second most common human arboviral infection after dengue virus. A new real-time RT-PCR was developed for OROV and reassortant species. The new OROV rRT-PCR proved linear across 6-7 orders of magnitude with a lower limit of 95%
Alejandra, Rojas +12 more
openaire +2 more sources
Sample-to-Answer and Routine Real-Time RT-PCR [PDF]
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is spreading all over the world and has caused millions of deaths. Several sample-to-answer platforms, including Cepheid Xpert Xpress SARS-CoV-2 (Xpert Xpress), have received emergency use authorization for SARS-CoV-2 nucleic acid detection as a point of care test in the United States ...
Donghua Wen +5 more
openaire +2 more sources
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis [PDF]
Molecular assays are rapid, sensitive and specific, and have become central to diagnosing rabies. PCR based assays have been utilized for decades to confirm rabies diagnosis but have only recently been accepted by the OIE (World Organisation for Animal Health) as a primary method to detect rabies infection.
Marston, Denise A +6 more
openaire +3 more sources
Real-time quantitative RT-PCR primers.
Real-time quantitative RT-PCR primers.
Gabriel O. Akerele (11714547) +5 more
core +1 more source
Intravitam Diagnosis of Rabies From Saliva by Nested RT-PCR and Real Time PCR
Nested reverse transcription (RT)-PCR and SYBR Green real time PCR protocols for the intravitam detection of rabies virus genomic RNA were tested with clinical samples for the first time from rabies suspected animals (n=12). With SYBR Green real time PCR,
Ambika Kaw +5 more
doaj +1 more source
Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon [PDF]
PCR inhibition by nucleic acid extracts is a well known yet poorly described phenomenon. Inhibition assessment generally depends on the assumption that inhibitors affect all PCR reactions to the same extent; i.e.
Miller Robert F +20 more
core +1 more source

