Results 51 to 60 of about 18,885,351 (305)

Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. [PDF]

, 2008
peer-reviewedBackground: A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, Kentucky and ...
Sheila McGuinness, McCabe, Evonne, Paul Whyte, Geraldine Duffy, McGuinness, Sheila, Barry, Thomas, Thomas Barry, Edel O'Regan, Séamus Fanning, Duffy, Geraldine, Whyte, Paul, O'Regan, Edel, Evonne McCabe, Catherine Burgess, Burgess, Catherine, Fanning, Seamus   +15 more
core   +1 more source

A real-time PCR assay to estimate Leishmania chagasi load in its natural sand fly vector Lutzomyia longipalpis [PDF]

, 2008
Leishmania chagasi, transmitted mainly by Lutzomyia longipalpis sand flies, causes visceral leishmaniasis and atypical cutaneous leishmaniasis in Latin America.
Bates, Paul A., Maingon, Rhayza D.C., Ranasinghe, Shalindra, Ranasinghe, S., Rogers, Matthew E., Hamilton, J. G. C., Maingon, Rhayza DC, Rogers, Matthew E, Bates, Paul, Hamilton, James G C, Maingon, Rhayza D C, Hamilton, James GC, Maingon, R. D. C., Hamilton, James G.C., Bates, Paul A   +14 more
core   +1 more source

Missed detections of influenza A(H1)pdm09 by real-time RT–PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam

Western Pacific Surveillance and Response, 2019
Introduction: There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR.
Hoang Vu Mai Phuong, Ung Thi Hong Trang, Nguyen Le Khanh Hang, Nguyen Thanh Thuy, Le Thi Thanh, Nguyen Vu Son, Nguyen Phuong Anh, Tran Thi Thu Huong, Vuong Duc Cuong, Le Quynh Mai   +9 more
doaj   +1 more source

Detection of SARS Coronavirus in Plasma by Real-Time RT-PCR

New England Journal of Medicine, 2003
To the Editor: The identification and sequencing of a novel coronavirus1 associated with the recently described severe acute respiratory syndrome (SARS)2 have permitted the development of antibody-...
Grant, PR, Garson, JA, Tedder, RS, Chan, PKS, Tam, JS, Sung, JJY   +5 more
openaire   +3 more sources

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Primers used for real-time RT-PCR

, 2016
Primers used for real-time RT ...
Kohta Ohnishi (387891), Akira Murakami (331566), Yoko Igarashi (3044799), Kazuhiro Irie (387902)   +3 more
core   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

MODERN LABORATORY DIAGNOSTIC METHODS IN VETERINARY MEDICINE AND THE POSSIBILITY OF ITS APPLICATION

Archives of Veterinary Medicine, 2010
One of the greatest challenges of modern laboratory diagnosis is the selection of methods for fast, highly sensitive and specific detection of the infective agents.
Tamaš Petrović, Maja Velhner, Jelena Petrović, Igor Stojanov, Živoslav Grgić, Sava Lazić   +5 more
doaj   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source