Results 41 to 50 of about 625,783 (265)

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil

Revista da Sociedade Brasileira de Medicina Tropical, 2016
: INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance.
Marilia Farignoli Romeiro, William Marciel de Souza, Aline Lavado Tolardo, Luiz Carlos Vieira, Tatiana Elias Colombo, Victor Hugo Aquino, Maurício Lacerda Nogueira, Luiz Tadeu Moraes Figueiredo   +7 more
doaj   +1 more source

Clinical Evaluation of Three Commercial RT-PCR Kits for Routine COVID-19 Diagnosis

Pathogens, 2022
Amongst the multiple ways to diagnose coronavirus disease-2019 (COVID-19), reverse transcription polymerase chain reaction (RT-PCR) remains the reference gold standard, providing fast and accurate results.
Rifat Tasnim Juthi, Saiful Arefeen Sazed, Md Fahad Zamil, Mohammad Shafiul Alam   +3 more
doaj   +1 more source

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

Molecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh, Nitzan Medina‐Itzhaki, Milena Chekov, Eden Gal‐Swisa, Roba Gabesh‐Wahabi, Inbar Savyon, Inna Naroditsky, Hilary A. Kenny, Basem Fares, Lina Korsensky, Einav Girsh, Liron Berger, Ruth Perets   +12 more
wiley   +1 more source

Fast, Sensitive and Specific Detection of Thailand orthohantavirus and Its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay

Viruses, 2019
Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific
Vololoniaina Raharinosy, Jean-Michel Heraud, Harinirina Aina Rabemananjara, Sandra Telfer, Danielle Aurore Doll Rakoto, Claudia Filippone, Jean-Marc Reynes   +6 more
doaj   +1 more source

Evaluation of Rapid Antigen Test Results and Real-time Reverse Transcription Polymerase Chain Reaction for the Laboratory Diagnosis of SARS-CoV-2

Mediterranean Journal of Infection, Microbes and Antimicrobials, 2023
Introduction: Real-time reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard method for the diagnosis of Severe acute respiratory syndrome-Coronavirus-2 (SARS-CoV-2) infection.
Yeliz TANRIVERDİ ÇAYCI, Zeliha SEYFİ, Kübra HACIEMİNOĞLU ÜLKER, Demet GÜR VURAL, Kemal BİLGİN, Hızır Ufuk AKDEMİR, Esra AKYÜZ ÖZKAN, Asuman BİRİNCİ   +7 more
doaj   +1 more source

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source

Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Emerging Infectious Diseases, 2020
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance ...
Jesse J. Waggoner, Victoria Stittleburg, Renee Pond, Youssef Saklawi, Malaya K. Sahoo, Ahmed Babiker, Laila Hussaini, Colleen S. Kraft, Benjamin A. Pinsky, Evan J. Anderson, Nadine Rouphael   +10 more
doaj   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

A novel method for real time quantitative RT-PCR. [PDF]

Genome Research, 1996
A novel approach to quantitative reverse transcriptase polymerase chain reaction (QC RT-PCR) using real time detection and the 5' nuclease assay has been developed. Cystic fibrosis transmembrane transductance regulator (CFTR) target mRNA is reverse transcribed, amplified, detected, and quantitated in real time.
U E, Gibson, C A, Heid, P M, Williams
openaire   +2 more sources