Results 21 to 30 of about 18,885,351 (305)

Development of an RNA Extraction Protocol for Norovirus from Raw Oysters and Detection by qRT-PCR and Droplet-Digital RT-PCR

Foods, 2021
Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR ...
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irène Iugovaz, Neda Nasheri   +4 more
doaj   +1 more source

Real-time RT-PCR normalisation; strategies and considerations [PDF]

Genes & Immunity, 2005
Real-time RT-PCR has become a common technique, no longer limited to specialist core facilities. It is in many cases the only method for measuring mRNA levels of vivo low copy number targets of interest for which alternative assays either do not exist or lack the required sensitivity.
J, Huggett, K, Dheda, S, Bustin, A, Zumla   +3 more
openaire   +2 more sources

A Parylene Real Time PCR Microdevice [PDF]

, 2010
The polymerase chain reaction (PCR) is a powerful biochemical assay that is used in virtually all biochemical labs. By specifically amplifying a small sample of DNA, this technique is useful in the fields of paternity testing, forensics, and virus ...
Quach, Quoc Chan (Brandon)
core   +1 more source

Transcriptional Changes in Response to Arbuscular Mycorrhiza Development in the Model Plant Medicago truncatula

Molecular Plant-Microbe Interactions, 2003
Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf, Katja Manthey, Jasmin Doll, Andreas M. Perlick, Burkhard Linke, Thomas Bekel, Folker Meyer, Philipp Franken, Helge Küster, Franziska Krajinski   +9 more
doaj   +1 more source

Real-Time Quantitative RT-PCR: Design, Calculations, and Statistics [PDF]

The Plant Cell, 2009
Two recent letters to the editor of The Plant Cell ([Gutierrez et al., 2008][1]; [Udvardi et al., 2008][2]) highlighted the importance of following correct experimental protocol in quantitative RT-PCR (qRT-PCR).
Rieu, I., Powers, S. J.
openaire   +2 more sources

Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance

Memorias do Instituto Oswaldo Cruz, 2012
In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes ...
Márcia Gonçalves de Castro, Rita Maria Ribeiro Nogueira, Ana Maria Bispo de Filippis, Anielly Alves Ferreira, Monique da Rocha Queiroz Lima, Nieli Rodrigues da Costa Faria, Fernanda de Bruycker Nogueira, Jaqueline Bastos Santos Simões, Priscila Conrado Guerra Nunes, Simone Alves Sampaio, Ricardo Lourenço-de-Oliveira, Flávia Barreto dos Santos   +11 more
doaj   +1 more source

Real-time quantification of microRNAs by stem-loop RT-PCR [PDF]

Nucleic Acids Research, 2005
A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis. Stem-loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related miRNAs that differ by as little as one nucleotide ...
Chen, Caifu, Ridzon, Dana A., Broomer, Adam J., Zhou, Zhaohui, Lee, Danny H., Nguyen, Julie T., Barbisin, Maura, Xu, Nan Lan, Mahuvakar, Vikram R., Andersen, Mark R., Lao, Kai Qin, Livak, Kenneth J., Guegler, Karl J.   +12 more
openaire   +2 more sources

Comparison of real-time PCR, conventional PCR and RT-lamp for the detection of Coconut Cadang-Cadang Viroid variant in oil palm

, 2023
Real-time quantitative polymerase chain reaction (real-time PCR) was designed for the detection of oil palm Coconut Cadang-cadang Viroid (CCCVd) 246 nt variant.
Roslan, Nur Diyana, Vadamalai, Ganesan, Idris, Abu Seman, Kong, Lih Ling, Sundram, Shamala   +4 more
core   +1 more source

Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies [PDF]

, 2009
De Gregoris TB, Borra M, Biffali E, et al. Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. BMC Molecular Biology.
De Gregoris, Tristano Bacchetti, Clare, Anthony S., Biffali, Elio, Elio Biffali, Thomas Bekel, Bekel, Thomas, Richard R Kirby, Borra, Marco, J Grant Burgess, Marco Borra, Burgess, J. Grant, Bacchetti De Gregoris, Tristano, Anthony S Clare, Kirby, Richard R., Tristano Bacchetti De Gregoris   +14 more
core   +1 more source

Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize [PDF]

, 2012
Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a
Atoui, Ali, Kallassy, Mireille, El Khoury, André, Lebrihi, Ahmed   +3 more
core   +1 more source