Results 21 to 30 of about 625,783 (265)
Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus
浙江大学学报. 农业与生命科学版, 2010 A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV).
LI Bin +4 more
doaj +1 more source
Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology [PDF]
Archives of Biological Sciences, 2008 Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the
Majić Dragana B. +4 more
doaj +1 more source
Towards a Systematic Validation of References in Real-Time RT-PCR [PDF]
The Plant Cell, 2008 Real-time RT-PCR (also known as quantitative RT-PCR [qRT-PCR]) is a powerful tool for quantifying gene expression, combining both high sensitivity and specificity with efficient signal detection. It has relatively recently begun to be used to monitor gene expression in plants (reviewed in [Gachon ...
Laurent, Gutierrez +4 more
openaire +2 more sources
SAS programs for real-time RT-PCR having multiple independent samples
BioTechniques, 2004 Relative real-time reverse transcription PCR (RT-PCR) has become an important tool for quantifying changes in messenger RNA (mRNA) populations following differential development or stimulation of tissues or cells. However, the best methods for conducting
Peyton Cook +4 more
doaj +1 more source
Quantitation of rabbit cytokine mRNA by real-time RT-PCR [PDF]
Cytokine, 2007 Fundamental understanding of rabbit immunology and the use of the rabbit as a disease model have long been hindered by the lack of immunological assays specific to this species. In the present study, we sought to develop a method to quantitate cytokine expression in rabbit cells and tissues.
Charmie, Godornes +4 more
openaire +2 more sources
Pathogens, 2022 In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature
Marta Luigi +7 more
doaj +1 more source
Annals of Clinical Microbiology, 2019 Background: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread of infection. The main
+4 more
doaj +1 more source
Sensors, 2012 Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV.
Jin-Guang Yang +7 more
doaj +1 more source
Detection of SARS Coronavirus in Plasma by Real-Time RT-PCR
New England Journal of Medicine, 2003 To the Editor: The identification and sequencing of a novel coronavirus1 associated with the recently described severe acute respiratory syndrome (SARS)2 have permitted the development of antibody-...
Grant, PR +5 more
openaire +3 more sources
Western Pacific Surveillance and Response, 2019 Introduction: There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR.
Hoang Vu Mai Phuong +9 more
doaj +1 more source