Results 21 to 30 of about 19,661,854 (307)
Real-time RT-PCR normalisation; strategies and considerations [PDF]
Genes & Immunity, 2005 Real-time RT-PCR has become a common technique, no longer limited to specialist core facilities. It is in many cases the only method for measuring mRNA levels of vivo low copy number targets of interest for which alternative assays either do not exist or lack the required sensitivity.
J, Huggett +3 more
openaire +2 more sources
, 2022 Real-time quantitative polymerase chain reaction (real-time PCR) was designed for the detection of oil palm Coconut Cadang-cadang Viroid (CCCVd) 246 nt variant.
Roslan, Nur Diyana +5 more
core +1 more source
Molecular Plant-Microbe Interactions, 2003 Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf +9 more
doaj +1 more source
Real-Time RT-PCR for the Detection of Lyssavirus Species [PDF]
Journal of Veterinary Medicine, 2014 The causative agents of rabies are single-stranded, negative-sense RNA viruses in the genus Lyssavirus of Rhabdoviridae, consisting of twelve classified and three as yet unclassified species including classical rabies virus (RABV). Highly neurotropic RABV causes rapidly progressive encephalomyelitis with nearly invariable fatal outcome.
Deubelbeiss, A. +4 more
openaire +2 more sources
A Parylene Real Time PCR Microdevice [PDF]
, 2010 The polymerase chain reaction (PCR) is a powerful biochemical assay that is used in virtually all biochemical labs. By specifically amplifying a small sample of DNA, this technique is useful in the fields of paternity testing, forensics, and virus ...
Quach, Quoc Chan (Brandon)
core +1 more source
Real-Time Quantitative RT-PCR: Design, Calculations, and Statistics [PDF]
The Plant Cell, 2009 Two recent letters to the editor of The Plant Cell ([Gutierrez et al., 2008][1]; [Udvardi et al., 2008][2]) highlighted the importance of following correct experimental protocol in quantitative RT-PCR (qRT-PCR).
Rieu, I., Powers, S. J.
openaire +2 more sources
Memorias do Instituto Oswaldo Cruz, 2012 In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes ...
Márcia Gonçalves de Castro +11 more
doaj +1 more source
Real-time quantification of microRNAs by stem-loop RT-PCR [PDF]
Nucleic Acids Research, 2005 A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis. Stem-loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related miRNAs that differ by as little as one nucleotide ...
Chen, Caifu +12 more
openaire +2 more sources
Primers and probe sequences used for real-time RT-PCR and RT-PCR.
, 2022 Primers and probe sequences used for real-time RT-PCR and RT-PCR.
Zhiyu Xiao (6210914) +15 more
core +1 more source