Results 11 to 20 of about 625,783 (265)
Real-time RT-PCR normalisation; strategies and considerations [PDF]
Genes & Immunity, 2005 Real-time RT-PCR has become a common technique, no longer limited to specialist core facilities. It is in many cases the only method for measuring mRNA levels of vivo low copy number targets of interest for which alternative assays either do not exist or lack the required sensitivity.
J, Huggett +3 more
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Molecular Plant-Microbe Interactions, 2003 Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf +9 more
doaj +1 more source
Real-Time Quantitative RT-PCR: Design, Calculations, and Statistics [PDF]
The Plant Cell, 2009 Two recent letters to the editor of The Plant Cell ([Gutierrez et al., 2008][1]; [Udvardi et al., 2008][2]) highlighted the importance of following correct experimental protocol in quantitative RT-PCR (qRT-PCR).
Rieu, I., Powers, S. J.
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Real-time quantification of microRNAs by stem-loop RT-PCR [PDF]
Nucleic Acids Research, 2005 A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis. Stem-loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related miRNAs that differ by as little as one nucleotide ...
Chen, Caifu +12 more
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Memorias do Instituto Oswaldo Cruz, 2012 In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes ...
Márcia Gonçalves de Castro +11 more
doaj +1 more source
Real-time RT-PCR for the detection and quantitation of Oropouche virus [PDF]
Diagnostic Microbiology and Infectious Disease, 2020 Oropouche virus (OROV) causes an acute, systemic febrile illness, and in certain regions of South America, this represents the second most common human arboviral infection after dengue virus. A new real-time RT-PCR was developed for OROV and reassortant species. The new OROV rRT-PCR proved linear across 6-7 orders of magnitude with a lower limit of 95%
Alejandra, Rojas +12 more
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Molecular Plant-Microbe Interactions, 2004 The KUP family of potassium transporters in plants is large but poorly characterized. We isolated and characterized the first KUP transporter from a legume, LjKUP of Lotus japonicus.
Guilhem Desbrosses +3 more
doaj +1 more source
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis [PDF]
Journal of Visualized Experiments, 2019 Molecular assays are rapid, sensitive and specific, and have become central to diagnosing rabies. PCR based assays have been utilized for decades to confirm rabies diagnosis but have only recently been accepted by the OIE (World Organisation for Animal Health) as a primary method to detect rabies infection.
Marston, Denise A +6 more
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Journal of Infection in Developing Countries, 2021 Introduction: Acute Chagas disease involving reactivation can occur after organ transplant, and follow-up by direct parasitological or molecular methods is essential for monitoring the parasitic load in such patients. In contrast, there is a little data
Juliana Jesus Guimarães Ferreira +9 more
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Intravitam Diagnosis of Rabies From Saliva by Nested RT-PCR and Real Time PCR
Brazilian Journal of Veterinary Pathology, 2012 Nested reverse transcription (RT)-PCR and SYBR Green real time PCR protocols for the intravitam detection of rabies virus genomic RNA were tested with clinical samples for the first time from rabies suspected animals (n=12). With SYBR Green real time PCR,
Ambika Kaw +5 more
doaj +1 more source