Real-Time RT-PCR for the Detection of Lyssavirus Species [PDF]
Journal of Veterinary Medicine, 2014 The causative agents of rabies are single-stranded, negative-sense RNA viruses in the genus Lyssavirus of Rhabdoviridae, consisting of twelve classified and three as yet unclassified species including classical rabies virus (RABV). Highly neurotropic RABV causes rapidly progressive encephalomyelitis with nearly invariable fatal outcome.
Deubelbeiss, A. +4 more
openaire +5 more sources
Comparative Performance of Digital PCR and Real-Time RT-PCR in Respiratory Virus Diagnostics [PDF]
VirusesBackground: Respiratory viral infections pose a major global health burden, and molecular diagnostics such as Real-Time RT-PCR have revealed frequent co-infections. However, precise quantification of viral RNA remains challenging.
Irene Bianconi +5 more
doaj +2 more sources
Enhanced rabies surveillance in roadkill specimens by real-time RT-PCR. [PDF]
PLoS Neglected Tropical DiseasesRoadkill specimens are an important source of samples for enhanced rabies surveillance (ERS) in areas where other methods of sample collection may not be practical.
Crystal M Gigante +8 more
doaj +2 more sources
Application of standard and molecular methods for the diagnosis of newcastle disease [PDF]
Archives of Biological Sciences, 2012 Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007 ...
Vidanović D. +6 more
doaj +1 more source
Quantitative real-time RT-PCR – a perspective [PDF]
Journal of Molecular Endocrinology, 2005 Abstract The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel ...
S A, Bustin +3 more
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Evaluating an MRNA based body fluid identification test using sybr green fluorescent dye and real-time PCR [PDF]
, 2011 The requirement to have more definitive and wider ranging body fluid identification (BFI) tests has resulted in a range of mRNA based real-time PCR BFI assays utilising Taqman fluorescent dye.
Connolly, Jo-Ann, Williams, Graham
core +3 more sources
Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay [PDF]
Veterinary World, 2013 Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV). Materials and Methods: The BTV serotype 21 isolate (KMNO7) was isolated from Andhra Pradesh and propagated ...
Gaya Prasad +5 more
doaj +1 more source
استفاده از Real time RT-PCR جهت شناسایی ویروس زبان آبی ایران [PDF]
Archives of Razi Institute, 2011 طی سال های 1388-1389 از روش real time RT-PCR و RT-PCRمعمولی جهت شناسایی ویروس زبان آبی در 310 گوسفند مشکوک به بیماری زبان آبی استفاده شد. قطعاتS1 (ژن پلی مراز) و S10 (ژن NS3 ) بعنوان ژنهای حراست شده در گروه سرمی زبان آبی به ترتیب توسط rRT-PCRوRT-PCR ...
عبدالحمید شوشتری +3 more
doaj +1 more source
تشخیص سریع و دقیق ویروس تب برفکی با روش PCR Real time در نمونههای بالینی [PDF]
Archives of Razi Institute, 2012 طی سالهای 1390-1389، از روش PCR Real-time برای ردیابی RNA ویروس تب برفکی در 147 نمونه اپیتلیوم از دامهای مشکوک به بیماری تب برفکی که از کانونهای مختلف اخذ گردیده بود استفاده شد.
همایون مهروانی +3 more
doaj +1 more source
Real-time RT-PCR diagnostics of virus causing COVID-19
Фармакоэкономика, 2020 Aim: the study was aimed to develop a reagent kit for the real-time RT-PCR diagnostics of virus causing COVID-19.Materials and Methods. Three target sites were chosen in the genome SARS-CoV-2.
E. V. Goncharova +6 more
doaj +1 more source