Results 61 to 70 of about 19,661,854 (307)
Determination of RNA stability using reverse transcription real-time PCR
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2008 The aim of this study was to verify an effect of RNA storage in different laboratory conditions. Especially, this work was focused on the importance of using diethylpyrocarbonate (DEPC) treated water for storage of isolated RNA.
Karel Bílek +2 more
doaj +1 more source
Outbreak of hepatitis E virus infection in Darfur, Sudan: effectiveness of real-time reverse transcription-PCR analysis of dried blood spots [PDF]
, 2009 Biological samples collected in refugee camps during an outbreak of hepatitis E were used to compare the accuracy of hepatitis E virus RNA amplification by real-time reverse transcription-PCR (RT-PCR) for sera and dried blood spots (concordance of 90.6%).
Mérens, Audrey +3 more
core +1 more source
Molecular Oncology, EarlyView.The proposed mechanism of action for the CDK12/13 inhibitor and cyclin K degrader, CT7439. CDK12/13 inhibition interrupts transcription elongation, leading to increased DNA damage that results in cell death. This agent is a potentially novel treatment option for patients with colorectal cancer. Created in BioRender. Cyclin‐dependent kinase (CDK) 12 and
Wylie K. Watlington +10 more
wiley +1 more source
Revista da Sociedade Brasileira de Medicina Tropical, 2016 : INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance.
Marilia Farignoli Romeiro +7 more
doaj +1 more source
Clinical Evaluation of Three Commercial RT-PCR Kits for Routine COVID-19 Diagnosis
Pathogens, 2022 Amongst the multiple ways to diagnose coronavirus disease-2019 (COVID-19), reverse transcription polymerase chain reaction (RT-PCR) remains the reference gold standard, providing fast and accurate results.
Rifat Tasnim Juthi +3 more
doaj +1 more source
Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies [PDF]
, 2009 De Gregoris TB, Borra M, Biffali E, et al. Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. BMC Molecular Biology.
De Gregoris, Tristano Bacchetti +14 more
core +1 more source
Cancer‐associated mutations in endometriosis reframe a benign disease through molecular oncology
Molecular Oncology, EarlyView.This review aims to comprehensively analyse cancer‐associated somatic mutations (CAMs) present in endometriotic lesions, emphasizing their biological roles, spatial distribution and implications for translational applications in medicine. By contextualizing a benign state within a genomic framework, this analysis seeks to establish its value as a ...
Clarissa Mujacic +15 more
wiley +1 more source
Viruses, 2019 Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific
Vololoniaina Raharinosy +6 more
doaj +1 more source
Mediterranean Journal of Infection, Microbes and Antimicrobials, 2023 Introduction: Real-time reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard method for the diagnosis of Severe acute respiratory syndrome-Coronavirus-2 (SARS-CoV-2) infection.
Yeliz TANRIVERDİ ÇAYCI +7 more
doaj +1 more source
The primers used for RT-PCR and real-time RT-PCR.
, 2012 The primers used for RT-PCR and real-time RT-PCR.
Na Li (6550) +10 more
core +1 more source