Results 41 to 50 of about 1,220,561 (394)

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis [PDF]

Journal of Visualized Experiments, 2019
Molecular assays are rapid, sensitive and specific, and have become central to diagnosing rabies. PCR based assays have been utilized for decades to confirm rabies diagnosis but have only recently been accepted by the OIE (World Organisation for Animal Health) as a primary method to detect rabies infection.
Denise A, Marston, Daisy L, Jennings, Nikki C, MacLaren, Daniel, Dorey-Robinson, Anthony R, Fooks, Ashley C, Banyard, Lorraine M, McElhinney   +6 more
openaire   +2 more sources

Evaluation of Trypanosoma cruzi parasitic load by real-time PCR and blood culture in long-term kidney transplant recipients

Journal of Infection in Developing Countries, 2021
Introduction: Acute Chagas disease involving reactivation can occur after organ transplant, and follow-up by direct parasitological or molecular methods is essential for monitoring the parasitic load in such patients. In contrast, there is a little data
Juliana Jesus Guimarães Ferreira, Eros Antonio de Almeida, Gláucia Elisete Barbosa Marcon, Rodrigo Gonçalves Lima, Mariane Barroso Pereira, Fernanda Ramos Gadelha, Marilda Mazzali, Luiz Cláudio Martins, Jamiro Silva Wanderley, Sandra Cecília Botelho Costa   +9 more
doaj   +1 more source

A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses [PDF]

, 2010
In order to establish an accurate, ready-to-use assay for simultaneous detection of Eastern equine encephalitis virus (EEEV) and Western equine encephalitis virus (WEEV), we developed one duplex TaqMan real-time reverse transcriptase polymerase chain ...
Xiaoping Kang, Yuchang Li, Hong Liu, Fang Lin, Xuyu Cai, Tingting Sun, Guohui Chang, Qingyu Zhu, Yinhui Yang   +8 more
core   +1 more source

Molecular assays for detection of human metapneumovirus [PDF]

, 2003
The recent description of the respiratory pathogen human metapneumovirus (hMPV) has highlighted a deficiency in current diagnostic techniques for viral agents associated with acute lower respiratory tract infections.
Mackay, Ian M., Jacob, Kevin C., Woolhouse, Daniel, Waller, Katharine, Syrmis, Melanie W., Whiley, David M., Siebert, David J., Nissen, Michael, Sloots, Theo P.   +8 more
core   +2 more sources

An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development

BMC Plant Biology, 2006
BackgroundAccuracy in quantitative real-time RT-PCR is dependent on high quality RNA, consistent cDNA synthesis, and validated stable reference genes for data normalization.
K. E. Reid, Niclas Olsson, J. Schlosser, F. Peng, Steven T. Lund   +4 more
semanticscholar   +1 more source

Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens

International Journal of Molecular Sciences, 2020
The pandemic novel coronavirus infection, Coronavirus Disease 2019 (COVID-19), has affected at least 190 countries or territories, with 465,915 confirmed cases and 21,031 deaths.
C. Yip, Chi-chun Ho, J. Chan, K. To, Helen Shuk-Ying Chan, S. Wong, Kit-Hang Leung, Agnes Yim-Fong Fung, A. C. Ng, Zijiao Zou, A. Tam, T. Chung, Kwok-Hung Chan, I. Hung, V. Cheng, O. Tsang, S. Tsui, K. Yuen   +17 more
semanticscholar   +1 more source

Intravitam Diagnosis of Rabies From Saliva by Nested RT-PCR and Real Time PCR

Brazilian Journal of Veterinary Pathology, 2012
Nested reverse transcription (RT)-PCR and SYBR Green real time PCR protocols for the intravitam detection of rabies virus genomic RNA were tested with clinical samples for the first time from rabies suspected animals (n=12). With SYBR Green real time PCR,
Ambika Kaw, Charan K. Singh, Naresh K Sood, Bhupinder S Sandhu, Deepak Deka, Sumedha Awahan   +5 more
doaj   +1 more source

Detection of dengue virus in saliva and urine by real time RT-PCR [PDF]

, 2010
Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time
Telma R Poloni, Anibal S Oliveira, Helda L Alfonso, Larissa R Galvao, Alberto A Amarilla, Dimair F Poloni, Luiz T Figueiredo, Victor H Aquino   +7 more
core   +1 more source

Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus

浙江大学学报. 农业与生命科学版, 2010
A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV).
LI Bin, SU Han, WU Cui-ping, ZHOU Ming-hua, AN Yu-lin   +4 more
doaj   +1 more source

Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology [PDF]

Archives of Biological Sciences, 2008
Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the
Majić Dragana B., Samardžić Jelena T., Milisavljević Mira Đ., Krstić A.M., Maksimović Vesna R.   +4 more
doaj   +1 more source