Results 51 to 60 of about 1,220,561 (394)

Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia () Growth Hormone Receptor Type I [PDF]

, 2009
Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for tilapia growth hormone receptor type I is ...
Rodrí, Cá, Lugo Juana, Oliva Aymé, Morales Antonio, Estrada Mario   +5 more
core   +1 more source

Species-specific Real Time-PCR primers/probe systems to identify fish parasites of the genera Anisakis, Pseudoterranova and Hysterothylacium (Nematoda: Ascaridoidea) [PDF]

, 2018
Ascaridoid nematodes belonging to the genera Anisakis and Pseudoterranova are heteroxenous parasites, involving marine mammals as definitive hosts in their life-cycles, whereas crustaceans (krill), fish and squids acting as intermediate/paratenic hosts.
Colantoni, Alessandra, Gay, Melanie, Levsen, Arne, Mattiucci, Simonetta, Nascetti, Giuseppe, Paoletti, Michela   +5 more
core   +1 more source

Towards a Systematic Validation of References in Real-Time RT-PCR [PDF]

The Plant Cell, 2008
Real-time RT-PCR (also known as quantitative RT-PCR [qRT-PCR]) is a powerful tool for quantifying gene expression, combining both high sensitivity and specificity with efficient signal detection. It has relatively recently begun to be used to monitor gene expression in plants (reviewed in [Gachon ...
Laurent, Gutierrez, Mélanie, Mauriat, Jérôme, Pelloux, Catherine, Bellini, Olivier, Van Wuytswinkel   +4 more
openaire   +2 more sources

SAS programs for real-time RT-PCR having multiple independent samples

BioTechniques, 2004
Relative real-time reverse transcription PCR (RT-PCR) has become an important tool for quantifying changes in messenger RNA (mRNA) populations following differential development or stimulation of tissues or cells. However, the best methods for conducting
Peyton Cook, Chunxiao Fu, Morgen Hickey, Eun-Soo Han, Kenton S. Miller   +4 more
doaj   +1 more source

Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato

Pathogens, 2022
In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature
Marta Luigi, Ariana Manglli, Antonio Tiberini, Sabrina Bertin, Luca Ferretti, Anna Taglienti, Francesco Faggioli, Laura Tomassoli   +7 more
doaj   +1 more source

A Step Forward in Molecular Diagnostics of Lyssaviruses – Results of a Ring Trial among European Laboratories [PDF]

, 2013
Rabies is a lethal and notifiable zoonotic disease for which diagnostics have to meet the highest standards. In recent years, an evolution was especially seen in molecular diagnostics with a wide variety of different detection methods published ...
A Orlowska, A Vos, A Vos, Anita Huovilainen, Anthony R. Fooks, B Hoffmann, B Hoffmann, B Hoffmann, Bernard Brochier, Bernd Hoffmann, CM Freuling, Conrad M. Freuling, D David, Denise A. Marston, DT Hayman, E Picard-Meyer, E Robardet, EM Black, Engbert A. Kooi, Florence Cliquet, GJ Hughes, IV Kuzmin, J Coertse, JE Echevarria, JE Whitby, Jean Mooney, Kerstin Wernike, L Dacheux, L Dacheux, M Biswal, M Fischer, M Ito, Marcin Smreczak, Martin Beer, Mats Isaksson, Melina Fischer, Mihai Turcitu, ML East, Orhan Aylan, P Crepin, P De Benedictis, Peter Hostnik, PR Heaton, PR Wakeley, S Vazquez-Moron, S Wacharapluesadee, S Wacharapluesadee, SA Nadin-Davis, Sandra Revilla-Fernández, Sonia Vázquez-Morón, T Müller, T Nagaraj, Thomas B. Rasmussen, Thomas Müller, V Shankar, W Saengseesom, Wanda Markotter   +56 more
core   +6 more sources

Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

Nature Communications, 2018
The emergence and spread of Zika virus (ZIKV) presented a challenge to the diagnosis of ZIKV infections in areas with transmission of dengue (DENV) and chikungunya (CHIKV) viruses.
G. Santiago, Jesús Vázquez, S. Courtney, Katia Y Matias, Lauren E. Andersen, Candimar Colón, A. Butler, Rebecca M. Roulo, John Bowzard, Julie M. Villanueva, J. Muñoz-Jordán   +10 more
semanticscholar   +1 more source

Quantitation of rabbit cytokine mRNA by real-time RT-PCR [PDF]

Cytokine, 2007
Fundamental understanding of rabbit immunology and the use of the rabbit as a disease model have long been hindered by the lack of immunological assays specific to this species. In the present study, we sought to develop a method to quantitate cytokine expression in rabbit cells and tissues.
Charmie, Godornes, Brandon Troy, Leader, Barbara J, Molini, Arturo, Centurion-Lara, Sheila A, Lukehart   +4 more
openaire   +2 more sources

Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection [PDF]

, 2017
Objectives: Feline leukaemia virus (FeLV), a gamma retrovirus, causes diseases of the feline haematopoietic system that are invariably fatal. Rapid and accurate testing at the point-of-need (PON) supports prevention of virus spread and management of ...
Anis, Eman, Chang, Hsiao-Fen G., Dunbar, Dawn, Graham, Elizabeth M., Lee, Fu-Chun, Lee, Pei-Yu A., Tsai, Yun-Long, Wang, Hwa-Tang T., Wilkes, Rebecca P.   +8 more
core   +1 more source

Multiple assays in a real-time RT-PCR SARS-CoV-2 panel can mitigate the risk of loss of sensitivity by new genomic variants during the COVID-19 outbreak

International Journal of Infectious Diseases, 2020
Objectives In this study, five SARS-CoV-2 PCR assay panels were evaluated against the accumulated genetic variability of the virus to assess the effect on sensitivity of the individual assays.
Luis Peñarrubia, M. Ruiz, Roberto Porco, Sonia N. Rao, Martí Juanola-Falgarona, Davide Manissero, Marta López-Fontanals, J. Pareja   +7 more
semanticscholar   +1 more source