Results 51 to 60 of about 19,862,753 (307)

Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil

open access: yesRevista da Sociedade Brasileira de Medicina Tropical, 2016
: INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance.
Marilia Farignoli Romeiro   +7 more
doaj   +1 more source

Clinical Evaluation of Three Commercial RT-PCR Kits for Routine COVID-19 Diagnosis

open access: yesPathogens, 2022
Amongst the multiple ways to diagnose coronavirus disease-2019 (COVID-19), reverse transcription polymerase chain reaction (RT-PCR) remains the reference gold standard, providing fast and accurate results.
Rifat Tasnim Juthi   +3 more
doaj   +1 more source

Real-time RT-PCR primer sequences and reaction parameters.

open access: yes, 2019
Real-time RT-PCR primer sequences and reaction parameters.
Dapeng Zhang (89211)   +4 more
core   +1 more source

Gut microbiota alterations in patients with non‐small‐cell lung cancer undergoing chemoradiotherapy

open access: yesMolecular Oncology, EarlyView.
We investigated the impact of concurrent chemoradiotherapy (CRT) on the gut microbiota in patients with locally advanced non‐small‐cell lung cancer. Overall gut microbiota composition remained largely stable throughout CRT while antibiotic exposure may influence microbial diversity.
Hanne Marte Nymoen   +19 more
wiley   +1 more source

Fast, Sensitive and Specific Detection of Thailand orthohantavirus and Its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay

open access: yesViruses, 2019
Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific
Vololoniaina Raharinosy   +6 more
doaj   +1 more source

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

open access: yesMolecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico   +5 more
wiley   +1 more source

Determination of RNA stability using reverse transcription real-time PCR

open access: yesActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2008
The aim of this study was to verify an effect of RNA storage in different laboratory conditions. Especially, this work was focused on the importance of using diethylpyrocarbonate (DEPC) treated water for storage of isolated RNA.
Karel Bílek   +2 more
doaj   +1 more source

Evaluation of Rapid Antigen Test Results and Real-time Reverse Transcription Polymerase Chain Reaction for the Laboratory Diagnosis of SARS-CoV-2

open access: yesMediterranean Journal of Infection, Microbes and Antimicrobials, 2023
Introduction: Real-time reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard method for the diagnosis of Severe acute respiratory syndrome-Coronavirus-2 (SARS-CoV-2) infection.
Yeliz TANRIVERDİ ÇAYCI   +7 more
doaj   +1 more source

List of primers used for RT-PCR and real-time RT-PCR.

open access: yes, 2013
List of primers used for RT-PCR and real-time RT-PCR.
Kazuaki Nonaka (112499)   +8 more
core   +1 more source

Outbreak of hepatitis E virus infection in Darfur, Sudan: effectiveness of real-time reverse transcription-PCR analysis of dried blood spots [PDF]

open access: yes, 2009
Biological samples collected in refugee camps during an outbreak of hepatitis E were used to compare the accuracy of hepatitis E virus RNA amplification by real-time reverse transcription-PCR (RT-PCR) for sera and dried blood spots (concordance of 90.6%).
Mérens, Audrey   +3 more
core   +1 more source

Home - About - Disclaimer - Privacy