Results 71 to 80 of about 1,220,561 (394)

Simultaneous detection and differentiation of dengue virus serotypes 1-4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay [PDF]

, 2011
Background Rapid identification and differentiation of mosquito-transmitted flaviviruses in acute-phase sera of patients and field-caught vector mosquitoes are important for the prediction and prevention of large-scale epidemics.
Shuhua Li, Meiyu Fang, Bin Zhou, Hongxia Ni, Qiuxia Shen, Hongwei Zhang, Yifang Han, Jianhua Yin, Wenjun Chang, Guozhang Xu, Guangwen Cao   +10 more
core   +1 more source

Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon

BMC Molecular Biology, 2005
BackgroundSalmonid fishes are among the most widely studied model fish species but reports on systematic evaluation of reference genes in qRT-PCR studies is lacking.ResultsThe stability of six potential reference genes was examined in eight tissues of ...
P. Olsvik, K. Lie, A. Jordal, T. Nilsen, I. Hordvik   +4 more
semanticscholar   +1 more source

Phenotypic and genotypic characterization of single circulating tumor cells in the follow‐up of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Single circulating tumor cells (sCTCs) from high‐grade serous ovarian cancer patients were enriched, imaged, and genomically profiled using WGA and NGS at different time points during treatment. sCTCs revealed enrichment of alterations in Chromosomes 2, 7, and 12 as well as persistent or emerging oncogenic CNAs, supporting sCTC identity.
Carolin Salmon, Rui P. L. Neves, Nikolas H. Stoecklein, Sven‐Thorsten Liffers, Jens Siveke, Jan D. Kuhlmann, Pauline Wimberger, Paul Buderath, Rainer Kimmig, Sabine Kasimir‐Bauer   +9 more
wiley   +1 more source

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR [PDF]

, 2016
Real Time Polymerase Chain Reaction also known as quantitative PCR (q-PCR) is a widely used tool in microbial ecology to quantify gene abundances of taxonomic and functional groups in environmental samples.
McKew, Boyd A, Smith, Cindy J, Tatti, Enrico, Whitby, Corrine   +3 more
core   +2 more sources

Dammarenediol II enhances etoposide‐induced apoptosis by targeting O‐GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer

Molecular Oncology, EarlyView.
Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee, Byung‐Cheol Han, Gi‐Bang Koo, Jihye Park, Mijin Kwon, Young Bin Park, Jae‐Mun Choi, Seung‐Ho Lee, Sangho Roh   +8 more
wiley   +1 more source

Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control [PDF]

, 2011
<p>The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories.
Beer, Martin, Blome, Sandra, Bonilauri, Paolo, Fernández-Piñero, Jovita, Greiser-Wilke, Irene, Haegeman, Andy, Hoffmann, Bernd, Isaksson, Mats, Koenen, Frank, Le Potier, Marie-Frederique, LeBlanc, Neil, Leifer, Immanuel, Loeffen, Willie, Rasmussen, Thomas Bruun, Stadejek, Tomasz, Ståhl, Karl, Tignon, Marylène, Uttenthal, Åse, van der Poel, Wim   +18 more
core   +7 more sources

Primers and probe design and precision assessment of the real time RT-PCR assay in Coxsackievirus A10 and enterovirus detection

Data in Brief, 2017
This data article contains data related to the research article entitled “Rapid detection of enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay” (Chen at al., 2017) [1]. Primers and probe sequence design are among
Jingfang Chen, Rusheng Zhang, Xinhua Ou, Dong Yao, Zheng Huang, Linzhi Li, Biancheng Sun   +6 more
doaj   +1 more source

Virus Culture and Real-time RT-PCR in Identifying Influenza Viruses From Influenza-like Illness Cases in Indonesia 2007-2008 [PDF]

, 2011
Introduction: From the influenza-like illness (ILI) surveillance in Indonesia, we learned that there was disagreement between virus culture and reverse trancriptase polymerase chain reaction (RT-PCR).
Indalao, I. L. (Irene), Pawestri, H. A. (Hana), Setiawaty, V. (Vivi), Subangkit, S. (Subangkit)   +3 more
core  

Colorectal cancer‐derived FGF19 is a metabolically active serum biomarker that exerts enteroendocrine effects on mouse liver

Molecular Oncology, EarlyView.
Meta‐transcriptome analysis identified FGF19 as a peptide enteroendocrine hormone associated with colorectal cancer prognosis. In vivo xenograft models showed release of FGF19 into the blood at levels that correlated with tumor volumes. Tumoral‐FGF19 altered murine liver metabolism through FGFR4, thereby reducing bile acid synthesis and increasing ...
Jordan M. Beardsley, Michael W. Rohr, Joseph A. Goode, Sai Preethi Nakkina, Jignesh G. Parikh, Deborah A. Altomare   +5 more
wiley   +1 more source

Appling real time RT-PCR for bluetongue virus detection in Iran

Archives of Razi Institute, 2011
During 2009-10, real time RT-PCR and conventional RT-PCR techniques Used for detecting BTVs RNA in310 blood samples. For real time and gel based RT-PCR segment-1 and segment-10 selected as conservegenes to search any BTV strains. Using these methods, 58 (
Shoshtari, A., Jeirani, F.,, Mahravani, H.,, Azimi, S.M.   +3 more
doaj