Results 61 to 70 of about 19,862,753 (307)
Molecular Oncology, EarlyView.This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault +12 more
wiley +1 more source
Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
Emerging Infectious Diseases, 2020 Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance ...
Jesse J. Waggoner +10 more
doaj +1 more source
Table 1. Primers used for real-time RT-PCR and RT-PCR.
, 2014 Table 1. Primers used for real-time RT-PCR and RT-PCR.
Claus Hellerbrand (94992) +6 more
core +1 more source
Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer
Molecular Oncology, EarlyView.Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba +5 more
wiley +1 more source
The development and application of the two real-time RT-PCR assays to detect the pathogen of HFMD. [PDF]
PLoS ONE, 2013 Large-scale Hand, Foot, and Mouth Disease (HFMD) outbreaks have frequently occurred in China since 2008, affecting more than one million children and causing several hundred children deaths every year. The pathogens of HFMD are mainly human enteroviruses
Aili Cui +7 more
doaj +1 more source
The primers used for RT-PCR and real-time RT-PCR.
, 2012 The primers used for RT-PCR and real-time RT-PCR.
Na Li (6550) +10 more
core +1 more source
Molecular Oncology, EarlyView.LVRF (lymphatic and vascular resistance factor) is a VEGFA splice variant upregulated in response to anti‐angiogenic therapy that fosters tumor adaptation through enhanced vascularization, lymphangiogenesis, and metastatic dissemination. Targeting LVRF with fully human neutralizing antibodies disrupts these processes and limits resistant tumor growth ...
Marion David +12 more
wiley +1 more source
A novel method for real time quantitative RT-PCR. [PDF]
Genome Research, 1996 A novel approach to quantitative reverse transcriptase polymerase chain reaction (QC RT-PCR) using real time detection and the 5' nuclease assay has been developed. Cystic fibrosis transmembrane transductance regulator (CFTR) target mRNA is reverse transcribed, amplified, detected, and quantitated in real time.
U E, Gibson, C A, Heid, P M, Williams
openaire +2 more sources
Oligonucleotide primer sequences for RT-PCR and real -time quantitative RT-PCR.
, 2012 Oligonucleotide primer sequences for RT-PCR and real -time quantitative RT-PCR.
Claudia Müller (19978) +6 more
core +1 more source
Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
Molecular Oncology, EarlyView.We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source