Results 31 to 40 of about 19,862,753 (307)

SAS programs for real-time RT-PCR having multiple independent samples

open access: yesBioTechniques, 2004
Relative real-time reverse transcription PCR (RT-PCR) has become an important tool for quantifying changes in messenger RNA (mRNA) populations following differential development or stimulation of tissues or cells. However, the best methods for conducting
Peyton Cook   +4 more
doaj   +1 more source

Primers used for real-time RT-PCR

open access: yes, 2016
Primers used for real-time RT ...
Kohta Ohnishi (387891)   +3 more
core   +1 more source

Towards a Systematic Validation of References in Real-Time RT-PCR [PDF]

open access: yesThe Plant Cell, 2008
Real-time RT-PCR (also known as quantitative RT-PCR [qRT-PCR]) is a powerful tool for quantifying gene expression, combining both high sensitivity and specificity with efficient signal detection. It has relatively recently begun to be used to monitor gene expression in plants (reviewed in [Gachon ...
Laurent, Gutierrez   +4 more
openaire   +2 more sources

Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens

open access: yesAnnals of Clinical Microbiology, 2019
Background: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread of infection. The main
  +4 more
doaj   +1 more source

Quantitation of rabbit cytokine mRNA by real-time RT-PCR [PDF]

open access: yesCytokine, 2007
Fundamental understanding of rabbit immunology and the use of the rabbit as a disease model have long been hindered by the lack of immunological assays specific to this species. In the present study, we sought to develop a method to quantitate cytokine expression in rabbit cells and tissues.
Charmie, Godornes   +4 more
openaire   +2 more sources

Development of a One-Step Immunocapture Real-Time RT-PCR Assay for Detection of Tobacco Mosaic Virus in Soil

open access: yesSensors, 2012
Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV.
Jin-Guang Yang   +7 more
doaj   +1 more source

Real-time RT-PCR primer sequences.

open access: yes, 2018
Real-time RT-PCR primer sequences.
Hiroshi Nagahisa (5946449)   +1 more
core   +1 more source

Missed detections of influenza A(H1)pdm09 by real-time RT–PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam

open access: yesWestern Pacific Surveillance and Response, 2019
Introduction: There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR.
Hoang Vu Mai Phuong   +9 more
doaj   +1 more source

Detection of SARS Coronavirus in Plasma by Real-Time RT-PCR

open access: yesNew England Journal of Medicine, 2003
To the Editor: The identification and sequencing of a novel coronavirus1 associated with the recently described severe acute respiratory syndrome (SARS)2 have permitted the development of antibody-...
Grant, PR   +5 more
openaire   +3 more sources

Inhibition of cyclin‐dependent kinases 12/13 using CT7439 as a treatment for colorectal cancer with CDK12 upregulation

open access: yesMolecular Oncology, EarlyView.
The proposed mechanism of action for the CDK12/13 inhibitor and cyclin K degrader, CT7439. CDK12/13 inhibition interrupts transcription elongation, leading to increased DNA damage that results in cell death. This agent is a potentially novel treatment option for patients with colorectal cancer. Created in BioRender. Cyclin‐dependent kinase (CDK) 12 and
Wylie K. Watlington   +10 more
wiley   +1 more source

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