Results 31 to 40 of about 637,318 (299)

Quantitation of rabbit cytokine mRNA by real-time RT-PCR [PDF]

open access: yesCytokine, 2007
Fundamental understanding of rabbit immunology and the use of the rabbit as a disease model have long been hindered by the lack of immunological assays specific to this species. In the present study, we sought to develop a method to quantitate cytokine expression in rabbit cells and tissues.
Charmie, Godornes   +4 more
openaire   +2 more sources

Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens

open access: yesAnnals of Clinical Microbiology, 2019
Background: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread of infection. The main
  +4 more
doaj   +1 more source

Development of a One-Step Immunocapture Real-Time RT-PCR Assay for Detection of Tobacco Mosaic Virus in Soil

open access: yesSensors, 2012
Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV.
Jin-Guang Yang   +7 more
doaj   +1 more source

Detection of SARS Coronavirus in Plasma by Real-Time RT-PCR

open access: yesNew England Journal of Medicine, 2003
To the Editor: The identification and sequencing of a novel coronavirus1 associated with the recently described severe acute respiratory syndrome (SARS)2 have permitted the development of antibody-...
Grant, PR   +5 more
openaire   +3 more sources

Missed detections of influenza A(H1)pdm09 by real-time RT–PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam

open access: yesWestern Pacific Surveillance and Response, 2019
Introduction: There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR.
Hoang Vu Mai Phuong   +9 more
doaj   +1 more source

Real-time RT-PCR validation.

open access: yes, 2015
* CI: confidence interval CV: coefficient of variation ND: Not done.Real-time RT-PCR validation.
Emmanuel Breard (5664328)   +4 more
core   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

open access: yesMolecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce   +11 more
wiley   +1 more source

Real-time RT-PCR primer sequences.

open access: yes, 2018
Real-time RT-PCR primer sequences.
Hiroshi Nagahisa (5946449)   +1 more
core   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

open access: yesMolecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano   +6 more
wiley   +1 more source

MODERN LABORATORY DIAGNOSTIC METHODS IN VETERINARY MEDICINE AND THE POSSIBILITY OF ITS APPLICATION

open access: yesArchives of Veterinary Medicine, 2010
One of the greatest challenges of modern laboratory diagnosis is the selection of methods for fast, highly sensitive and specific detection of the infective agents.
Tamaš Petrović   +5 more
doaj   +1 more source

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