Results 31 to 40 of about 1,220,561 (394)

Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples [PDF]

, 2005
Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for
Al Rashid S.T., Cloak O.M., Hani E.C., Hunt J.M., Linton D., Manser P.A., Munroe D., Ng L.-K., Penner J.L., Totten P.A., Wilson D.L.   +10 more
core   +1 more source

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Genome Biology, 2002
BackgroundGene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes.
J. Vandesompele, K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, F. Speleman   +6 more
semanticscholar   +1 more source

Diagnostic performance between CT and initial real-time RT-PCR for clinically suspected 2019 coronavirus disease (COVID-19) patients outside Wuhan, China

Respiratory Medicine, 2020
Introduction Chest CT is thought to be sensitive but less specific in diagnosing the 2019 coronavirus disease (COVID-19). The diagnostic value of CT is unclear.
Jianlong He, Lin Luo, Zhendong Luo, Jianxun Lyu, M. Ng, Xinping Shen, Z. Wen   +6 more
semanticscholar   +1 more source

Evaluation of two lyophilized molecular assays to rapidly detect foot-and-mouth disease virus directly from clinical samples in field settings [PDF]

, 2017
Accurate, timely diagnosis is essential for the control, monitoring and eradication of foot‐and‐mouth disease (FMD). Clinical samples from suspect cases are normally tested at reference laboratories.
Armson, B., Chepkwony, E., Cleaveland, S., Fowler, V.L., Howson, E.L.A., Kandusi, S., Kasanga, C.J., King, D.P., Lembo, T., Madi, M., Martin, P., Mioulet, V., Sallu, R., Siddle, A., Wood, J.   +14 more
core   +1 more source

On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]

, 2007
We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
core   +1 more source

Preanalytical Issues and Cycle Threshold Values in SARS-CoV-2 Real-Time RT-PCR Testing: Should Test Results Include These?

ACS Omega, 2021
Since the emergence of SARS-CoV-2 pandemic, clinical laboratories worldwide are overwhelmed with SARS-CoV-2 testing using the current gold standard: real-time reverse-transcription polymerase chain reaction (RT-PCR) assays. The large numbers of suspected
I. Engelmann, E. K. Alidjinou, J. Ogiez, Quentin Pagneux, Sana Miloudi, Ilyes Benhalima, Mahdi Ouafi, F. Sane, D. Hober, A. Roussel, C. Cambillau, D. Devos, R. Boukherroub, S. Szunerits   +13 more
semanticscholar   +1 more source

Real-time RT-PCR for the detection and quantitation of Oropouche virus [PDF]

Diagnostic Microbiology and Infectious Disease, 2020
Oropouche virus (OROV) causes an acute, systemic febrile illness, and in certain regions of South America, this represents the second most common human arboviral infection after dengue virus. A new real-time RT-PCR was developed for OROV and reassortant species. The new OROV rRT-PCR proved linear across 6-7 orders of magnitude with a lower limit of 95%
Alejandra, Rojas, Victoria, Stittleburg, Fátima, Cardozo, Nathen, Bopp, César, Cantero, Sanny, López, Cynthia, Bernal, Laura, Mendoza, Patricia, Aguilar, Benjamin A, Pinsky, Yvalena, Guillén, Malvina, Páez, Jesse J, Waggoner   +12 more
openaire   +2 more sources

Lotus japonicus LjKUP Is Induced Late During Nodule Development and Encodes a Potassium Transporter of the Plasma Membrane

Molecular Plant-Microbe Interactions, 2004
The KUP family of potassium transporters in plants is large but poorly characterized. We isolated and characterized the first KUP transporter from a legume, LjKUP of Lotus japonicus.
Guilhem Desbrosses, Claudia Kopka, Thomas Ott, Michael K. Udvardi   +3 more
doaj   +1 more source

Performance of SARS-CoV-2 rapid antigen test compared with real-time RT-PCR in asymptomatic individuals

medRxiv, 2021
Screening, testing and contact tracing plays a pivotal role in the control of COVID-19 pandemic. To carry out this strategy it is necessary to increase the testing capacity.
M. Peña, Manuel Ampuero, C. Garcés, A. Gaggero, Patricia García, M. Velasquez, Ricardo Luza, P. Alvarez, F. Paredes, Johanna Acevedo, M. Farfán, S. Solari, R. Soto-Rifo, F. Valiente-Echeverría   +13 more
semanticscholar   +1 more source

Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR [PDF]

, 2009
Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase (CesA) genes, csr2 and csr4, respectively were crossed.
Jacobsen, E., Olawole, O., Vincken, J.P., Visser, R.G.F.   +3 more
core   +2 more sources