Results 11 to 20 of about 689,799 (215)

Lotus japonicus LjKUP Is Induced Late During Nodule Development and Encodes a Potassium Transporter of the Plasma Membrane

open access: yesMolecular Plant-Microbe Interactions, 2004
The KUP family of potassium transporters in plants is large but poorly characterized. We isolated and characterized the first KUP transporter from a legume, LjKUP of Lotus japonicus.
Guilhem Desbrosses   +3 more
doaj   +1 more source

Evaluation of Trypanosoma cruzi parasitic load by real-time PCR and blood culture in long-term kidney transplant recipients

open access: yesJournal of Infection in Developing Countries, 2021
Introduction: Acute Chagas disease involving reactivation can occur after organ transplant, and follow-up by direct parasitological or molecular methods is essential for monitoring the parasitic load in such patients. In contrast, there is a little data
Juliana Jesus Guimarães Ferreira   +9 more
doaj   +1 more source

Intravitam Diagnosis of Rabies From Saliva by Nested RT-PCR and Real Time PCR

open access: yesBrazilian Journal of Veterinary Pathology, 2012
Nested reverse transcription (RT)-PCR and SYBR Green real time PCR protocols for the intravitam detection of rabies virus genomic RNA were tested with clinical samples for the first time from rabies suspected animals (n=12). With SYBR Green real time PCR,
Ambika Kaw   +5 more
doaj   +1 more source

Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus

open access: yes浙江大学学报. 农业与生命科学版, 2010
A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV).
LI Bin   +4 more
doaj   +1 more source

Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology [PDF]

open access: yesArchives of Biological Sciences, 2008
Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the
Majić Dragana B.   +4 more
doaj   +1 more source

Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato

open access: yesPathogens, 2022
In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature
Marta Luigi   +7 more
doaj   +1 more source

SAS programs for real-time RT-PCR having multiple independent samples

open access: yesBioTechniques, 2004
Relative real-time reverse transcription PCR (RT-PCR) has become an important tool for quantifying changes in messenger RNA (mRNA) populations following differential development or stimulation of tissues or cells. However, the best methods for conducting
Peyton Cook   +4 more
doaj   +1 more source

Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens

open access: yesAnnals of Clinical Microbiology, 2019
Background: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread of infection. The main
  +4 more
doaj   +1 more source

Development of a One-Step Immunocapture Real-Time RT-PCR Assay for Detection of Tobacco Mosaic Virus in Soil

open access: yesSensors, 2012
Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV.
Jin-Guang Yang   +7 more
doaj   +1 more source

Missed detections of influenza A(H1)pdm09 by real-time RT–PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam

open access: yesWestern Pacific Surveillance and Response, 2019
Introduction: There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR.
Hoang Vu Mai Phuong   +9 more
doaj   +1 more source

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