Results 11 to 20 of about 637,318 (299)

Primers used for Real-Time RT-PCR.

, 2013
Primers used for Real-Time RT-PCR.
Rebecca J. Kapphahn (282242), Neal D. Heuss (263583), Marcela Maldonado (282241), Marcia R. Terluk (282243), Ching Yuan (282244), Deborah A. Ferrington (282245), Dale S. Gregerson (263587)   +6 more
core   +6 more sources

Real-time RT-PCR normalisation; strategies and considerations [PDF]

Genes & Immunity, 2005
Real-time RT-PCR has become a common technique, no longer limited to specialist core facilities. It is in many cases the only method for measuring mRNA levels of vivo low copy number targets of interest for which alternative assays either do not exist or lack the required sensitivity.
J, Huggett, K, Dheda, S, Bustin, A, Zumla   +3 more
openaire   +2 more sources

Transcriptional Changes in Response to Arbuscular Mycorrhiza Development in the Model Plant Medicago truncatula

Molecular Plant-Microbe Interactions, 2003
Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf, Katja Manthey, Jasmin Doll, Andreas M. Perlick, Burkhard Linke, Thomas Bekel, Folker Meyer, Philipp Franken, Helge Küster, Franziska Krajinski   +9 more
doaj   +1 more source

Real-Time Quantitative RT-PCR: Design, Calculations, and Statistics [PDF]

The Plant Cell, 2009
Two recent letters to the editor of The Plant Cell ([Gutierrez et al., 2008][1]; [Udvardi et al., 2008][2]) highlighted the importance of following correct experimental protocol in quantitative RT-PCR (qRT-PCR).
Rieu, I., Powers, S. J.
openaire   +2 more sources

Real-time quantification of microRNAs by stem-loop RT-PCR [PDF]

Nucleic Acids Research, 2005
A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis. Stem-loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related miRNAs that differ by as little as one nucleotide ...
Chen, Caifu, Ridzon, Dana A., Broomer, Adam J., Zhou, Zhaohui, Lee, Danny H., Nguyen, Julie T., Barbisin, Maura, Xu, Nan Lan, Mahuvakar, Vikram R., Andersen, Mark R., Lao, Kai Qin, Livak, Kenneth J., Guegler, Karl J.   +12 more
openaire   +2 more sources

Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance

Memorias do Instituto Oswaldo Cruz, 2012
In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes ...
Márcia Gonçalves de Castro, Rita Maria Ribeiro Nogueira, Ana Maria Bispo de Filippis, Anielly Alves Ferreira, Monique da Rocha Queiroz Lima, Nieli Rodrigues da Costa Faria, Fernanda de Bruycker Nogueira, Jaqueline Bastos Santos Simões, Priscila Conrado Guerra Nunes, Simone Alves Sampaio, Ricardo Lourenço-de-Oliveira, Flávia Barreto dos Santos   +11 more
doaj   +1 more source

Real time RT-PCR primers.

, 2012
Real time RT-PCR primers.
Bo Xi (129419), Jie Zhang (64655), Jie Chen (5892), Jian Xue (145753), Yueran Zhao (145760), Dan He (145755), Mei Wang (130842)   +6 more
core   +2 more sources

Real-time RT-PCR primers.

, 2016
Real-time RT-PCR primers.
Pan Dong Ryu (2153392), Min Seok Song (2153389), So Yeong Lee (1792543), Seon Young Choi (546447)   +3 more
core   +2 more sources

Real-time RT-PCR for the detection and quantitation of Oropouche virus [PDF]

Diagnostic Microbiology and Infectious Disease, 2020
Oropouche virus (OROV) causes an acute, systemic febrile illness, and in certain regions of South America, this represents the second most common human arboviral infection after dengue virus. A new real-time RT-PCR was developed for OROV and reassortant species. The new OROV rRT-PCR proved linear across 6-7 orders of magnitude with a lower limit of 95%
Alejandra, Rojas, Victoria, Stittleburg, Fátima, Cardozo, Nathen, Bopp, César, Cantero, Sanny, López, Cynthia, Bernal, Laura, Mendoza, Patricia, Aguilar, Benjamin A, Pinsky, Yvalena, Guillén, Malvina, Páez, Jesse J, Waggoner   +12 more
openaire   +2 more sources

Lotus japonicus LjKUP Is Induced Late During Nodule Development and Encodes a Potassium Transporter of the Plasma Membrane

Molecular Plant-Microbe Interactions, 2004
The KUP family of potassium transporters in plants is large but poorly characterized. We isolated and characterized the first KUP transporter from a legume, LjKUP of Lotus japonicus.
Guilhem Desbrosses, Claudia Kopka, Thomas Ott, Michael K. Udvardi   +3 more
doaj   +1 more source