Results 11 to 20 of about 1,220,561 (394)
Real-time quantification of microRNAs by stem–loop RT–PCR [PDF]
Nucleic Acids Research, 2005 A novel microRNA (miRNA) quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones in terms of RT efficiency and specificity.
Caifu Chen +12 more
semanticscholar +3 more sources
Quantitative real-time RT-PCR--a perspective. [PDF]
Journal of Molecular Endocrinology, 2005 The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect ...
S. Bustin +3 more
semanticscholar +3 more sources
Real-time RT-PCR normalisation; strategies and considerations [PDF]
Genes & Immunity, 2005 Real-time RT-PCR has become a common technique, no longer limited to specialist core facilities. It is in many cases the only method for measuring mRNA levels of vivo low copy number targets of interest for which alternative assays either do not exist or lack the required sensitivity.
J. Huggett +3 more
semanticscholar +3 more sources
Detection of SARS coronavirus in plasma by real-time RT-PCR [PDF]
New England Journal of Medicine, 2003 To the Editor: The identification and sequencing of a novel coronavirus1 associated with the recently described severe acute respiratory syndrome (SARS)2 have permitted the development of antibody-...
Chan, PKS +5 more
core +4 more sources
A novel method for real time quantitative RT-PCR. [PDF]
Genome Research, 1996 A novel approach to quantitative reverse transcriptase polymerase chain reaction (QC RT-PCR) using real time detection and the 5' nuclease assay has been developed. Cystic fibrosis transmembrane transductance regulator (CFTR) target mRNA is reverse transcribed, amplified, detected, and quantitated in real time.
U E Gibson +2 more
openalex +4 more sources
Q-Gene: Processing Quantitative Real-time RT-PCR Data [PDF]
Bioinformatics, 2003 Abstract Summary: Q-Gene is an application for the processing of quantitative real-time RT–PCR data. It offers the user the possibility to freely choose between two principally different procedures to calculate normalized gene expressions as either means of Normalized Expressions or Mean Normalized Expressions.
P. Simon
semanticscholar +3 more sources
Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA
Journal of Virological Methods, 2011 Takeshi Noda, Yoshihiro Kawaoka
exaly +2 more sources
An optimized protocol for stepwise optimization of real-time RT-PCR analysis
Horticulture Research, 2021 Computational tool-assisted primer design for real-time reverse transcription (RT) PCR (qPCR) analysis largely ignores the sequence similarities between sequences of homologous genes in a plant genome.
Fangzhou Zhao +11 more
semanticscholar +1 more source
تشخیص سریع و دقیق ویروس تب برفکی با روش PCR Real time در نمونههای بالینی [PDF]
Archives of Razi Institute, 2012 طی سالهای 1390-1389، از روش PCR Real-time برای ردیابی RNA ویروس تب برفکی در 147 نمونه اپیتلیوم از دامهای مشکوک به بیماری تب برفکی که از کانونهای مختلف اخذ گردیده بود استفاده شد.
همایون مهروانی +3 more
doaj +1 more source
Real-time RT-PCR diagnostics of virus causing COVID-19
Фармакоэкономика, 2020 Aim: the study was aimed to develop a reagent kit for the real-time RT-PCR diagnostics of virus causing COVID-19.Materials and Methods. Three target sites were chosen in the genome SARS-CoV-2.
E. V. Goncharova +6 more
doaj +1 more source