Results 21 to 30 of about 19,862,753 (307)

Lotus japonicus LjKUP Is Induced Late During Nodule Development and Encodes a Potassium Transporter of the Plasma Membrane

open access: yesMolecular Plant-Microbe Interactions, 2004
The KUP family of potassium transporters in plants is large but poorly characterized. We isolated and characterized the first KUP transporter from a legume, LjKUP of Lotus japonicus.
Guilhem Desbrosses   +3 more
doaj   +1 more source

Evaluation of Trypanosoma cruzi parasitic load by real-time PCR and blood culture in long-term kidney transplant recipients

open access: yesJournal of Infection in Developing Countries, 2021
Introduction: Acute Chagas disease involving reactivation can occur after organ transplant, and follow-up by direct parasitological or molecular methods is essential for monitoring the parasitic load in such patients. In contrast, there is a little data
Juliana Jesus Guimarães Ferreira   +9 more
doaj   +1 more source

Real-time RT-PCR for the detection and quantitation of Oropouche virus [PDF]

open access: yesDiagnostic Microbiology and Infectious Disease, 2020
Oropouche virus (OROV) causes an acute, systemic febrile illness, and in certain regions of South America, this represents the second most common human arboviral infection after dengue virus. A new real-time RT-PCR was developed for OROV and reassortant species. The new OROV rRT-PCR proved linear across 6-7 orders of magnitude with a lower limit of 95%
Alejandra, Rojas   +12 more
openaire   +2 more sources

Primers used for real-time RT-PCR.

open access: yes, 2013
Primers used for real-time RT-PCR.
Jianfeng Wang (232744)   +11 more
core   +5 more sources

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis [PDF]

open access: yesJournal of Visualized Experiments, 2019
Molecular assays are rapid, sensitive and specific, and have become central to diagnosing rabies. PCR based assays have been utilized for decades to confirm rabies diagnosis but have only recently been accepted by the OIE (World Organisation for Animal Health) as a primary method to detect rabies infection.
Marston, Denise A   +6 more
openaire   +3 more sources

Real-time RT-PCR Primers.

open access: yes, 2012
Real-time RT-PCR Primers.
Taoping Shi (172485)   +12 more
core   +2 more sources

Intravitam Diagnosis of Rabies From Saliva by Nested RT-PCR and Real Time PCR

open access: yesBrazilian Journal of Veterinary Pathology, 2012
Nested reverse transcription (RT)-PCR and SYBR Green real time PCR protocols for the intravitam detection of rabies virus genomic RNA were tested with clinical samples for the first time from rabies suspected animals (n=12). With SYBR Green real time PCR,
Ambika Kaw   +5 more
doaj   +1 more source

Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus

open access: yes浙江大学学报. 农业与生命科学版, 2010
A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV).
LI Bin   +4 more
doaj   +1 more source

Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology [PDF]

open access: yesArchives of Biological Sciences, 2008
Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the
Majić Dragana B.   +4 more
doaj   +1 more source

Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato

open access: yesPathogens, 2022
In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature
Marta Luigi   +7 more
doaj   +1 more source

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