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Virology Journal, 2020 Background The Coronavirus disease 2019 (COVID-19) pandemic continues to spread across the world. Hence, there is an urgent need for rapid, simple, and accurate tests to diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection ...
Chutikarn Chaimayo +14 more
semanticscholar +1 more source
Real-Time RT-PCR for the Detection of Lyssavirus Species [PDF]
Journal of Veterinary Medicine, 2014 The causative agents of rabies are single-stranded, negative-sense RNA viruses in the genus Lyssavirus of Rhabdoviridae, consisting of twelve classified and three as yet unclassified species including classical rabies virus (RABV). Highly neurotropic RABV causes rapidly progressive encephalomyelitis with nearly invariable fatal outcome.
Deubelbeiss, Andrea +4 more
openaire +3 more sources
Real-time RT-PCR in COVID-19 detection: issues affecting the results
Expert Review of Molecular Diagnostics, 2020 Due to the rapid spread and increasing number of coronavirus disease 19 (COVID-19) cases caused by a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), rapid and accurat...
A. Tahamtan, A. Ardebili
semanticscholar +1 more source
Foods, 2021 Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR ...
Daniel Plante +4 more
doaj +1 more source
Emerging Microbes and Infections, 2021 SARS-CoV-2 has spread rapidly, causing deaths worldwide. In this study, we evaluated the performance of the BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, in nasopharyngeal specimens from individuals with symptoms of ...
Hsing-Yi Chung +16 more
semanticscholar +1 more source
Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques [PDF]
, 2019 Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops.
Alfaro-Fernandez A. +5 more
core +1 more source
Molecular Plant-Microbe Interactions, 2003 Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf +9 more
doaj +1 more source
Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR [PDF]
, 2017 Background Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses ...
Agoti, Charles N. +8 more
core +2 more sources
Real-Time Quantitative RT-PCR: Design, Calculations, and Statistics [PDF]
The Plant Cell, 2009 Two recent letters to the editor of The Plant Cell ([Gutierrez et al., 2008][1]; [Udvardi et al., 2008][2]) highlighted the importance of following correct experimental protocol in quantitative RT-PCR (qRT-PCR).
Rieu, I., Powers, S. J.
openaire +2 more sources
Memorias do Instituto Oswaldo Cruz, 2012 In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes ...
Márcia Gonçalves de Castro +11 more
doaj +1 more source