Results 21 to 30 of about 637,318 (299)
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis [PDF]
Journal of Visualized Experiments, 2019 Molecular assays are rapid, sensitive and specific, and have become central to diagnosing rabies. PCR based assays have been utilized for decades to confirm rabies diagnosis but have only recently been accepted by the OIE (World Organisation for Animal Health) as a primary method to detect rabies infection.
Marston, Denise A +6 more
openaire +3 more sources
Journal of Infection in Developing Countries, 2021 Introduction: Acute Chagas disease involving reactivation can occur after organ transplant, and follow-up by direct parasitological or molecular methods is essential for monitoring the parasitic load in such patients. In contrast, there is a little data
Juliana Jesus Guimarães Ferreira +9 more
doaj +1 more source
Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus
浙江大学学报. 农业与生命科学版, 2010 A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV).
LI Bin +4 more
doaj +1 more source
Primer sequences for real-time RT-PCR.
, 2016 Primer sequences for real-time RT-PCR.
Tadashi Kawakami (3202845) +4 more
core +3 more sources
Intravitam Diagnosis of Rabies From Saliva by Nested RT-PCR and Real Time PCR
Brazilian Journal of Veterinary Pathology, 2012 Nested reverse transcription (RT)-PCR and SYBR Green real time PCR protocols for the intravitam detection of rabies virus genomic RNA were tested with clinical samples for the first time from rabies suspected animals (n=12). With SYBR Green real time PCR,
Ambika Kaw +5 more
doaj +1 more source
Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology [PDF]
Archives of Biological Sciences, 2008 Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the
Majić Dragana B. +4 more
doaj +1 more source
Towards a Systematic Validation of References in Real-Time RT-PCR [PDF]
The Plant Cell, 2008 Real-time RT-PCR (also known as quantitative RT-PCR [qRT-PCR]) is a powerful tool for quantifying gene expression, combining both high sensitivity and specificity with efficient signal detection. It has relatively recently begun to be used to monitor gene expression in plants (reviewed in [Gachon ...
Laurent, Gutierrez +4 more
openaire +2 more sources
SAS programs for real-time RT-PCR having multiple independent samples
BioTechniques, 2004 Relative real-time reverse transcription PCR (RT-PCR) has become an important tool for quantifying changes in messenger RNA (mRNA) populations following differential development or stimulation of tissues or cells. However, the best methods for conducting
Peyton Cook +4 more
doaj +1 more source
Pathogens, 2022 In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature
Marta Luigi +7 more
doaj +1 more source