Results 141 to 150 of about 18,885,351 (305)
Diagnosing rotavirus A associated IID: using ELISA to identify a cut-off for real time RT-PCR [PDF]
, 2009 BackgroundThe use of RT-PCR for diagnosis of group A rotaviruses is increasing, but up to 14% of healthy individuals may be positive by RT-PCR. If RT-PCR is not well correlated with disease, rotavirus A may not always be the cause of illness in RT-PCR ...
Phillips, Gemma
core
Advanced Functional Materials, EarlyView.A 3D anisotropic hydrogel derived from heart extracellular matrix guides cytoskeletal alignment and nuclear remodeling in reprogrammed cardiomyocyte‐like cells. This study reveals how matrix alignment modulates nuclear envelope dynamics and chromatin state, triggering transcriptional and functional maturation.
Seung Ju Seo +7 more
wiley +1 more source
Real-Time PCR and Real-Time RT-PCR applications in food labelling and gene expression studies
, 2014 Polymerase chain reaction (PCR) as a scientific invention, has revolutionized molecular biology and led to real-time PCR and later, real-time reverse transcription PCR (Real-Time RT-PCR). These two techniques enable scientists to conduct PCR detection of
Malau-Aduli, AEO (15913250) +1 more
core
Primers Used for RT-PCR and Real-Time RT-PCR.
, 2014 ALP, alkaline phosphatase; RUNX2, runt-related transcription factor 2; ON, osteonectin; COL II, type II collagen; COL X, type X collagen; ACAN, aggrecan; LPL, lipoprotein lipase; PPAR-γ, peroxisome proliferator-activated receptor γ; OC, osteocalcin ...
Tianyong Hou (647259) +8 more
core +1 more source
Advanced Healthcare Materials, EarlyView.This review examines how cellular behavior is regulated by mechanical cues transmitted through soft biomaterials, from single‐cell mechanosensing to tissue‐level adaptation. It highlights why physiological relevance, rather than model complexity alone, is critical for translational mechanobiology and introduces a scoring framework linking material ...
Mathias Polz +9 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.Micro‐ and nano‐patterned elastin‐like recombinamer (ELR) hydrogels integrate bioactive matrix chemistry with defined surface topography. The combined cues promote rapid endothelial cell capture, alignment, and monolayer formation on soft substrates.
Jagoda Litowczenko +8 more
wiley +1 more source
Rapid and accurate diagnosis of Foot-and-mouth disease virus by Real-time PCR in field samples
Archives of Razi Institute, 2012 During 2010-2011, Real-time PCR procedure was used to detecting FMDV RNA on 147 epithelium samples from the field. In this survey, for Real-time PCR from 3D gene segment as conserve region selected for tracking all of seven serotypes FMDV.
Mahravani, H. +7 more
doaj
LN34 real-time RT-PCR protocol v2
, 2018 Purpose To describe the LN34 real-time RT-PCR assay procedure used for the qualitative detection of lyssavirus RNA in whole RNA extracted from brain tissue samples. The assay detects RNA from diverse lyssaviruses at varying concentrations [1].
Crystal M. Gigante +4 more
openaire +1 more source
Advanced Healthcare Materials, EarlyView.A spatially controlled embedded bioprinting strategy enables precise engineering of prevascularized multicellular spheroids within skin‐derived dECM bioinks. Optimized spheroid size, composition, and spacing promote vascular maturation, paracrine signaling, and tissue integration, resulting in enhanced angiogenesis, blood perfusion, and functional skin
Minjun Ahn +6 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.Schematic illustration of the proposed mechanism: PEG/RGD‐PSLs mimic apoptotic cells to engage PS receptors (notably CD300a), transducing an inhibitory signal that suppresses the MyD88/NF‐κB pathway, leading to global anti‐inflammatory and pro‐reparative effects.
Lele Wu +10 more
wiley +1 more source