, 2014 Real-time RT-PCR results for MHC class II genes Cd74 (A) and RT1-Da (B) regulated by unilateral STN-HFS. Lesion effect comparison (C) for both MHC class II genes.
Andrew Sharott (534318) +11 more
core +1 more source
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
The epidemiology of norovirus in England diagnostics, incidence and transmission [PDF]
Existing estimates of the incidence of infectious intestinal disease (IID) caused by norovirus in the community are based on electron microscopy or reverse transcription- polymerase chain reaction (RT-PCR).
Phillips, Gemma
core +1 more source
Norovirus in bivalve molluscs: development of a Real Time RT PCR protocol and its application for a viral contamination monitoring and for a bioaccumulation study [PDF]
, 2010 The Food Hygiene Package is a body of European Regulations laying down hygiene rules for foodstuffs produced in the EU and non-EU countries exporting to the EU.
Ventrone, Iole
core +1 more source
Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
FEBS Open Bio, EarlyView.We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source
Problems of Infectious and Parasitic DiseasesBackground: Human non-polio enteroviruses (NPEVs) are a diverse group of RNA viruses belonging to the genus Enterovirus within the subfamily Ensavirinae of the family Picornaviridae.
Irina Georgieva +6 more
doaj +1 more source
BioTechniques, 2005 Profiling studies using microarrays to measure messenger RNA (mRNA) expression frequently identify long lists of differentially expressed genes. Differential expression is often validated using real-time reverse transcription PCR (RT-PCR) assays.
Lynne V. Abruzzo +6 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Effect of solute stress and temperature on growth rate and TRI5 gene expression using real time RT-PCR in Fusarium graminearum from Spanish wheat [PDF]
, 2010 The objective of this work was to study the effect of ecophysiological factors on trichothecene gene expression and growth in Fusarium graminearum.
Magan, Naresh +4 more
core +1 more source
Establishment of real-time rt-pcr assay for detection of mrna mycobacterium tuberculosis
Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2011 Current laboratory methods for monitoring the response to therapy for tuberculosis (TB) rely on mycobacterial culture. Their clinical usefulness is therefore limited by the slow growth rate of Mycobacterium tuberculosis.
Hồ Thị Thanh Thủy +4 more
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