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Results 111 to 120 of about 19,661,854 (307)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu +2 more
wiley +1 more source

Primer sequences used in real time PCR and RT-PCR.

, 2014
Primer sequences used in real time PCR and RT-PCR.
Yeh Chen (680848) +4 more
core +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo +6 more
wiley +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang +10 more
wiley +1 more source

ISOLATION AND GENETIC IDENTIFICATION OF HUMAN NON-POLIO ENTEROVIRUSES AT THE NATIONAL REFERENCE LABORATORY OF ENTEROVIRUSES, BULGARIA

Problems of Infectious and Parasitic Diseases
Background: Human non-polio enteroviruses (NPEVs) are a diverse group of RNA viruses belonging to the genus Enterovirus within the subfamily Ensavirinae of the family Picornaviridae.
Irina Georgieva +6 more
doaj +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source

Primer sequences for real-time RT-PCR.

, 2016
Primer sequences for real-time RT-PCR.
Sung-Bae Kim (798272) +8 more
core +2 more sources

Validation of oligonucleotide microarray data using microfluidic low-density arrays: a new statistical method to normalize real-time RT-PCR data

BioTechniques, 2005
Profiling studies using microarrays to measure messenger RNA (mRNA) expression frequently identify long lists of differentially expressed genes. Differential expression is often validated using real-time reverse transcription PCR (RT-PCR) assays.
Lynne V. Abruzzo +6 more
doaj +1 more source

Oligonucleotide sequences for real-time RT-PCR.

, 2016
Oligonucleotide sequences for real-time RT-PCR.
Chin-Kai Tseng (276730) +6 more
core +1 more source

rt-pcr
real-time rt-pcr
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covid-19
real-time pcr
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conventional rt-pcr
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