EZH2 upregulation by ERα induces proliferation and migration of papillary thyroid carcinoma
BMC Cancer, 2019 Background The incidence of papillary thyroid carcinoma (PTC) has been increasing worldwide in recent years. Therefore, novel potential therapeutic targets for PTC are urgently needed.
Liqiong Xue +12 more
doaj +1 more source
Peach Kernel Oil Modulates Lipid Metabolism Disorder in ApoEa CRISPR/Cas9 Gene‐Disrupted Zebrafish
Food Frontiers, Volume 7, Issue 2, March 2026.In this study, we used CRISPR/Cas9 gene editing technology to establish a lipid metabolism disorder model of ApoEa mutant zebrafish. We applied peach kernel oil to this model and studied the mechanism of peach kernel oil regulating lipid disorder in combination with transcriptomics.
Lei Xia +14 more
wiley +1 more source
Real-time PCR for mRNA quantitation
BioTechniques, 2005 Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to ...
Marisa L. Wong, Juan F. Medrano
doaj +1 more source
Cloning and expression of porcine β1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen [PDF]
, 2014 Xenograft rejection of pigs organs with an engineered mutation in the GGTA-1 gene (GTKO) remains a predominantly antibody mediated process which is directed to a variety of non-Gal protein and carbohydrate antigens.
Byrne, GW +4 more
core +2 more sources
Cytotoxic effects of Oosporein isolated from endophytic fungus Cochliobolus kusanoi
Frontiers in Microbiology, 2015 In the present study, oosporein, a fungal toxic secondary metabolite known to be a toxic agent causing chronic disorders in animals, was isolated from fungus Cochliobolus kusanoi of Nerium oleander L.
Rmaesha eA +5 more
doaj +1 more source
Development and clinical validation of the Genedrive point-of-care test for qualitative detection of hepatitis C virus [PDF]
, 2018 Objective: Recently approved direct acting antivirals provide transformative therapies for chronic hepatitis C virus (HCV) infection. The major clinical challenge remains to identify the undiagnosed patients worldwide, many of whom live in low-income and
Ainsworth, Shaun +20 more
core +5 more sources
Accurate RT-qPCR gene expression analysis on cell culture lysates [PDF]
, 2012 Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of ...
Van Peer, Gert +2 more
core +1 more source
Longitudinal Analysis of Antibody Responses to Trachoma Antigens Before and After Mass Drug Administration. [PDF]
, 2014 Blinding trachoma, caused by the bacteria Chlamydia trachomatis, is a neglected tropical disease targeted for elimination by 2020. A major component of the elimination strategy is mass drug administration (MDA) with azithromycin.
Dize, Laura +10 more
core +2 more sources
Detection of economically important viruses in boar semen by quantitative RealTime PCR™ technology
Journal of Virological Methods, 2004 The objective of this study was to develop quantitative real-time polymerase chain reaction (ReTi-PCR) tests for the detection of five economically important viruses in swine semen namely, pseudorabies virus (PRV), classical swine fever virus (CSFV), foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and porcine reproductive and
van Rijn, P.A. +5 more
openaire +3 more sources
Neurotensin is a lipid-induced gastrointestinal peptide associated with visceral adipose tissue inflammation in obesity [PDF]
, 2018 Neurotensin (NT) is a 13-amino acid peptide localized in the neuroendocrine cells of the small intestine, which promotes fat absorption and fatty acids translocation in response to lipid ingestion. NT-knock-out mice fed with a high-fat diet are protected
Barchetta, Ilaria +11 more
core +2 more sources