Results 61 to 70 of about 32,209 (206)

Accurate RT-qPCR gene expression analysis on cell culture lysates [PDF]

open access: yes, 2012
Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of ...
Van Peer, Gert   +2 more
core   +1 more source

Salt Treatment Confers Protection Against Acute Carp Edema Virus Reinfection While Promoting Viral Persistence

open access: yesJournal of Fish Diseases, EarlyView.
ABSTRACT Carp edema virus (CEV) infects the common carp (Cyprinus carpio) and causes the lethal koi sleepy disease (KSD). Signs of KSD include respiratory, detoxification, and osmoregulatory difficulties. Salt treatment re‐equilibrates blood sodium levels and can save the fish. However, it is unclear whether these fish are immunized, remain chronically
Mikolaj Adamek   +12 more
wiley   +1 more source

Expression of VPAC1 in a murine model of allergic asthma [PDF]

open access: yes, 2013
Vasoactive intestinal polypeptide (VIP) is a putative neurotransmitter of the inhibitory non-adrenergic non-cholinergic nervous system and influences the mammalian airway function in various ways.
Braun, Armin   +4 more
core  

The Transcription Factor OsWRKY64 Interacts With OsART1 to Positively Regulate Al Resistance in Rice

open access: yesPlant Biotechnology Journal, EarlyView.
A proposed working model illustrating the cooperative regulation of Al tolerance by OsWRKY64 and OsART1 in rice. Under Al stress conditions in acidic soil, the transcription factor OsART1 directly binds to the promoter of OsWRKY64 to activate its transcription. The synthesized OsWRKY64 protein then physically interacts with OsART1 in the nucleus.
Changzhao Chen   +10 more
wiley   +1 more source

Severe pertussis infection in infants less than 6 months of age: clinical manifestations and molecular characterization [PDF]

open access: yes, 2017
We conducted a study to determine the main traits of pertussis among unimmunized infants less than 6 months of age. From August 2012 to March 2015, 141 nasopharyngeal aspirates (NPAs) were collected from infants with respiratory symptoms attending 2
Buttinelli, Gabriele   +12 more
core   +1 more source

Evaluation of Copan FLOQSwab for the molecular detection ofChlamydia trachomatisby Abbott RealTime CT PCR [PDF]

open access: yesActa Clinica Belgica, 2015
We evaluated Copan FLOQSwabs next to Abbott swabs for the detection of Chlamydia trachomatis (CT) by Abbott RealTime PCR.We collected 1062 paired swabs from female sex workers. The study was divided in two arms, according to the order of swab collection.If the Abbott swab was collected first, 501 couples were concordant and two discordant (Abbott ...
Coorevits, Liselotte   +5 more
openaire   +3 more sources

PLK4 is a potential therapeutic target in nonmelanoma skin cancers: Evidence from molecular and in vivo studies

open access: yesPhotochemistry and Photobiology, EarlyView.
Exposure to solar ultraviolet radiation is the main etiologic driver of nonmelanoma skin cancers (NMSCs), including basal cell (BCC) and cutaneous squamous cell carcinomas (cSCC), which are the most prevalent types of cancers in the US. In this study, we demonstrate that the serine/threonine kinase Polo‐like kinase 4 (PLK4) is overexpressed in NMSCs ...
Mary A. Ndiaye   +5 more
wiley   +1 more source

Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis

open access: yesInfection and Drug Resistance, 2017
Pilar Ruiz,1 Manuel Causse,2 Manuel Vaquero,1 Juan Bautista Gutierrez,1,2 Manuel Casal1,2 1Mycobacteria Reference Center, Department of Microbiology, Faculty of Medicine and Nursing, University of Córdoba, Córdoba, Spain; 2Microbiology ...
Ruiz P   +4 more
doaj  

Establishment and preliminary evaluation of a non-invasive fetal M blood group genotyping method by real-time PCR

open access: yesZhongguo shuxue zazhi
[Objective] To establish a method for the genotyping of fetal M blood group antigen by extracting cell-free fetal DNA (cff-DNA) from maternal plasma, so as to guide the management of M antigen-negative pregnant women with IgG anti-M antibody during ...
JIA Shuangshuang   +5 more
doaj   +1 more source

Genome-wide profiling of human cap-independent translation-enhancing elements. [PDF]

open access: yes, 2013
We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput ...
Briones, Natalia   +10 more
core  

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