Results 141 to 150 of about 2,453 (182)
Biochemical Engineering Journal, 2020 Abstract Engineering of enzymes without crystal structures and homologous structures is a great challenge for structure-based computational enzyme design. In this study, a computational strategy that combines protein structure prediction, sequence optimization, and molecular dynamics simulation was developed to improve the catalytic efficiency of the
Min Lin, Fei Wang, Yushan Zhu
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Metabolism and Toxicity Studies Supporting the Safety of Rebaudioside D
International Journal of Toxicology, 2013 Rebaudioside D (Reb D) is one of the several glycosides found in the leaves of Stevia rebaudiana (Bertoni) Bertoni (Compositae) which has been identified as a potential sweetener. The metabolism of Reb A and Reb D was evaluated in various in vitro matrices (simulated gastrointestinal fluids, rat liver microsomes, and rat cecal contents) and through ...
Andrey I. Nikiforov +3 more
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International Journal of Biological Macromolecules, 2020 Rebaudioside D is a promising sweetener due to its zero calorie and high sweetness. Here, a transglucosylase gene eugt11 from Oryza sativa was for the first time expressed in Pichia pastoris, and transformant XE-3 showed the highest expression levels in pH 5.5 BMMY media containing 0.75% methanol.
Zhenyang Wang +8 more
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Separation of rebaudioside D from steviol glycoside by frontal chromatography
, 2019 Steviol glycoside (SG) is the non-caloric food additive extracted from stevia rebaudiana leaves. It is a mixture of four major components, which are Rebaudioside A (RA), Rebaudioside C (RC), Rebaudioside D (RD) and Stevioside (STV). The RD is capable of providing 250-300 times sweetness than the sugar and giving the clean aftertaste.
Ka Chun Li
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Journal of Agricultural and Food Chemistry, 2019 In this study, a monoglucosyl rebaudioside A product was isolated from the mixture of glucosylated rebaudioside A obtained from the most reported and industrial used cyclodextrin glycosyl transferase, Toruzyme 3.0 L (CGTase, Toruzyme 3.0 L). The molecular structure of the monoglucosyl rebaudioside A was characterized using LC-MS/MS and methylation ...
Qingbin Guo +6 more
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Regulatory Toxicology and Pharmacology, 2013 The hydrolysis of the steviol glycosides rebaudioside A, B, D, and M, as well as of steviolbioside (a metabolic intermediate) to steviol was evaluated in vitro using human fecal homogenates from healthy donors under anaerobic conditions. Incubation of each of the rebaudiosides resulted in rapid hydrolysis to steviol. Metabolism was complete within 24h,
Sidd Purkayastha +5 more
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Synthesis of rebaudioside D, using glycosyltransferase UGTSL2 and in situ UDP-glucose regeneration
Food Chemistry, 2018 Steviol glycosides from Stevia rebaudiana leaves are used in stevia-based sweeteners for their intense sweetness and low calories. Rebaudioside D is present in leaves in minute quantities (∼0.4-0.5% w/w total dry weight), but it is ∼350 times sweeter than sucrose, and sweeter than the more abundant rebaudioside A and stevioside.
Liangliang Chen +8 more
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Journal of Agricultural and Food ChemistryRebaudioside M (Reb M), a zero-calorie sweetener with high sweetness, faces production challenges due to its low yield and purity. UGT76G1, a uridine diphosphate glucose (UDPG)-dependent glycosyltransferase, forms a β-1,3-glycosidic bond with rebaudioside D (Reb D) to produce Reb M but with an efficiency lower than that for stevioside (ST).
Fangwei Song +7 more
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Food Chemistry, 2014 Rebaudioside (Reb) D is a high intensity, natural sweetener that shows great potential for substituting sugar in sweetened beverages. However, Reb D is poorly water soluble, and thus, a solid dispersion technique was recently established to enhance its solubility.
Shintaro Pang +3 more
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