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Chapter 12 Quantitative Evaluation of Receptor-Mediated Endocytosis
1989Publisher Summary This chapter describes assays, experimental procedures, and analyses that are designed to allow a quantitative evaluation of the process of receptor-mediated endocytosis. It emphasizes on both a theoretical and a practical approach to these issues.
D. A. Owensby +2 more
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Receptor-mediated endocytosis of fucosylated neoglycoprotein by macrophages
Molecular and Cellular Biochemistry, 1996The characteristics of the recognition system involved in the receptor mediated endocytosis of the neoglycoprotein, fucose-human serum albumin (HSA) were studied. It was found that (i) fucose-HSA showed strong affinity binding and uptake by various macrophages; (ii) binding was specific for L-fucose and D-mannose; (iii) binding was found to be ...
Pijush K. Das +3 more
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A Regulatory Role for ARF6 in Receptor-Mediated Endocytosis
Science, 1995Adenosine diphosphate-ribosylation factor 6 (ARF6), ARF6 mutants, and ARF1 were transiently expressed in Chinese hamster ovary cells, and the effects on receptor-mediated endocytosis were assessed. Overexpressed ARF6 localized to the cell periphery and led to a redistribution of transferrin receptors to the cell surface and a decrease in the rate of ...
María Isabel Colombo +3 more
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Receptor-mediated endocytosis of keratinocyte growth factor
Journal of Cell Science, 1998ABSTRACT Keratinocyte growth factor (KGF) is a fibroblast growth factor which acts specifically on epithelial cells, regulating their proliferation and differentiation. KGF elicits its activity through binding to and activation of KGF receptor, a splicing transcript variant of fibroblast growth factor receptor 2 (FGFR2). Here we analyzed
Patrizia Mancini +7 more
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Regulation of receptor-mediated endocytosis by Rho and Rac
Nature, 1996Pinocytosis and membrane ruffling are among the earliest and most dramatic cellular responses to stimulation by growth factors or other mitogens. The small Ras-related G proteins Rho and Rac have a regulatory role in membrane ruffling and activated Rho has been shown to stimulate pinocytosis when microinjected into Xenopus oocytes. In contrast to these
Tsung-Hsein Chuang +4 more
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Receptor-mediated endocytosis of tuftsin by macrophage cells
Biochemical and Biophysical Research Communications, 1984A fluorescent analog of the phagocytosis stimulating peptide tuftsin was prepared by coupling tetramethyl rhodamine isothiocyanate to a C-terminal elongated derivative of tuftsin. This analog, Thr-Lys-Pro-Arg-Gly-Lys(N epsilon-tetramethyl rhodamine)-OH, was used to visualize tuftsin receptors on mice macrophage cells by fluorescent image ...
Philip A. Gottlieb +5 more
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Mathematical Modeling of Receptor-Mediated Endocytosis
1985For a number of reasons, receptor-mediated endocytosis provides an ideal system in which a biological process can be approached with mathematical modeling. Much progress has been made in the ability to define and measure individual steps in the complex multistep process of endocytosis, and the use of radiolabeled ligands makes quantitative kinetic data
Byron Goldstein +4 more
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Receptor-mediated endocytosis of trichosanthin in choriocarcinoma cells
Toxicology, 2003Trichosanthin (TCS) is a ribosome inactivating protein (RIP). It is generally believed that its many biological activities act through inhibition of ribosomes resulting in a decrease in protein synthesis. It has been hypothesized that the rate of entry of TCS into cells to reach ribosomes is an important factor in determining its biological activity ...
Siu-Cheung Tam, Wood Yee Chan, Hai Huang
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Ethanol and regulation of receptor-mediated endocytosis [PDF]
Hepatology, 1990 openaire +2 more sources