Results 131 to 140 of about 268,012 (165)
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Journal of Steroid Biochemistry, 1980
Abstract A controlled trypsinization procedure is reported which results in more efficient solubilization of the nuclear estrogen receptor from human endometrium and rat uterine tissue than does extraction with high salt (0.4 M KCl) alone. The trypsin-liberated nuclear receptors in both species sediment at 3–4S on both high and low salt gradients ...
Benita S. Katzenellenbogen +5 more
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Abstract A controlled trypsinization procedure is reported which results in more efficient solubilization of the nuclear estrogen receptor from human endometrium and rat uterine tissue than does extraction with high salt (0.4 M KCl) alone. The trypsin-liberated nuclear receptors in both species sediment at 3–4S on both high and low salt gradients ...
Benita S. Katzenellenbogen +5 more
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Nuclear and Cytoplasmic Estrogen Receptors in Ovine Endometrium During the Estrous Cycle1
Endocrinology, 1977Binding of estradiol to a specific endometrial cytoplasmic receptor and translocation of the estrogen-receptor complex to the nucleus was studied in ewes on days 0 (estrus), 3, 6 and 10 of the estrous cycle. In addition, total endometrial cytoplasmic and nuclear receptors were determined by use of the estradiol exchange assay.
K. Bruce Koligian, Fredrick Stormshak
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Journal of Steroid Biochemistry, 1985
An assay for the quantitation of cytoplasmic and nuclear glucocorticoid receptors in lymphoid tissue has been developed using controlled pore glass (CPG) beads. Soluble receptor--3H-steroid complex (cytosol or nuclear extract) is adsorbed quantitatively within the crevasses of porous glass beads.
Elwood V. Jensen +3 more
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An assay for the quantitation of cytoplasmic and nuclear glucocorticoid receptors in lymphoid tissue has been developed using controlled pore glass (CPG) beads. Soluble receptor--3H-steroid complex (cytosol or nuclear extract) is adsorbed quantitatively within the crevasses of porous glass beads.
Elwood V. Jensen +3 more
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Endocrinology, 1979
Nuclear and cytoplasmic estrogen receptors have been identified and characterized in the rabbit corpus luteum, and validated methods are described for the measurement of both unoccupied and total estrogen receptor. Binding was specific for the biologically active estrogens. Equilibrium binding analysis of cytoplasmic estrogen receptor yielded saturable,
Khe-Ching M. Yuh, Landis P. Keyes
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Nuclear and cytoplasmic estrogen receptors have been identified and characterized in the rabbit corpus luteum, and validated methods are described for the measurement of both unoccupied and total estrogen receptor. Binding was specific for the biologically active estrogens. Equilibrium binding analysis of cytoplasmic estrogen receptor yielded saturable,
Khe-Ching M. Yuh, Landis P. Keyes
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Molecular pathways involved in the transport of nuclear receptors from the nucleus to cytoplasm
The Journal of Steroid Biochemistry and Molecular Biology, 2018Nuclear receptors (NRs) are transcription regulators that direct the expression of many genes linked to cellular processes, such as proliferation, differentiation, and apoptosis. Additionally, some cellular events are also modulated by signaling pathways induced by NRs outside of the nucleus.
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European Journal of Cancer (1965), 1980
21 mastectomy specimens were investigated none of which had received preoperative radiation chemotherapy or endocrine therapy to determine the cytoplasmic and nuclear estradiol receptor content in different parts of human mammary carcinomas. The study found cytoplasmic-specific estradiol receptor values to vary widely but predictably throughout the ...
Sven A. Gustafsson +4 more
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21 mastectomy specimens were investigated none of which had received preoperative radiation chemotherapy or endocrine therapy to determine the cytoplasmic and nuclear estradiol receptor content in different parts of human mammary carcinomas. The study found cytoplasmic-specific estradiol receptor values to vary widely but predictably throughout the ...
Sven A. Gustafsson +4 more
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Nuclear‐cytoplasmic transport of EGFR involves receptor endocytosis, importin β1 and CRM1
Journal of Cellular Biochemistry, 2006AbstractMany receptor tyrosine kinases (RTKs) can be detected in the cell nucleus, such as EGFR, HER‐2, HER‐3, HER‐4, and fibroblast growth factor receptor. EGFR, HER‐2 and HER‐4 contain transactivational activity and function as transcription co‐factors to activate gene promoters. High EGFR in tumor nuclei correlates with increased tumor proliferation
Sheng-Chieh Hsu +5 more
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Biochemical Pharmacology, 1994
The signal responsible for the nuclear localization of the progesterone receptor has been characterized. The study of the mechanism of this nuclear localization has revealed that the receptor continuously shuttles between the nucleus and the cytoplasm.
Anne Guiochon-Mantel +4 more
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The signal responsible for the nuclear localization of the progesterone receptor has been characterized. The study of the mechanism of this nuclear localization has revealed that the receptor continuously shuttles between the nucleus and the cytoplasm.
Anne Guiochon-Mantel +4 more
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Archives of Biochemistry and Biophysics, 1986
In order to investigate the subcellular distribution of unoccupied 1,25-dihydroxyvitamin D3 receptors, highly purified cytoplasts and nucleoplasts were prepared from two kidney cell lines (PK1 and MDBK). This was accomplished utilizing the technique of enucleation by cytochalasin B and density gradient centrifugation.
Timothy A. Reinhardt +4 more
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In order to investigate the subcellular distribution of unoccupied 1,25-dihydroxyvitamin D3 receptors, highly purified cytoplasts and nucleoplasts were prepared from two kidney cell lines (PK1 and MDBK). This was accomplished utilizing the technique of enucleation by cytochalasin B and density gradient centrifugation.
Timothy A. Reinhardt +4 more
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Heat Shock Proteins and the Cytoplasmic-Nuclear Trafficking of Steroid Receptors
1994Transcription factors, such as steroid receptors, must move through the cytoplasm to the nucleus and subsequently within the nucleus in a precisely targeted manner. Experiments carried out twenty years ago in intact cells exposed to metabolic inhibitors suggested that glucocorticoid receptors (GR) that have entered the nucleus (then defined as the low ...
William B. Pratt, Lawrence C. Scherrer
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