Results 21 to 30 of about 551,717 (313)

PROTEIN BIOTECHNOLOGY: THE SIGNIFICANCE AND USE OF INDUSTRIAL POLYMER-DEGRADING ENZYMES [PDF]

open access: yesمجلة جامعة كركوك للعلوم الزراعية
Microorganisms are the primary source of industrial enzymes, with Bacillus and Aspergillus species being the leading producers. Bacillus strains have historically been used to synthesize commercially significant enzymes due to their status as GRAS ...
Mustafa Omar
doaj   +1 more source

DNA looping: the consequences and its control [PDF]

open access: yesCurr. Opin. Struct. Biol. 16, 344-350 (2006), 2006
The formation of DNA loops by proteins and protein complexes is ubiquitous to many fundamental cellular processes, including transcription, recombination, and replication. Here we review recent advances in understanding the properties of DNA looping in its natural context and how they propagate to the cellular behavior through gene regulation.
arxiv   +1 more source

Investigating BB0405 as a novel Borrelia afzelii vaccination candidate in Lyme borreliosis

open access: yesScientific Reports, 2021
BB0405 is a surface exposed Borrelia burgdorferi protein and its vaccination protected mice against B. burgdorferi infection. As BB0405 is highly conserved across different B.
M. J. Klouwens   +8 more
doaj   +1 more source

Complementary Theory of Evolutionary Genetics [PDF]

open access: yesarXiv, 2021
This theory seeks to define species and to explore evolutionary forces and genetic elements in speciation and species maintenance. The theory explains how speciation and species maintenance are caused by natural selection acting on non-Mendelian and Mendelian variation, respectively. The emergence and maintenance of species as groups of populations are
arxiv  

Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate

open access: yesIndonesian Journal of Biotechnology, 2005
Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the research was to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinant technology.
Erma Sulistyaningsih   +3 more
doaj   +1 more source

DNA looping in gene regulation: from the assembly of macromolecular complexes to the control of transcriptional noise [PDF]

open access: yesCurrent Opinion in Genetics & Development, 15, 136-144 (2005), 2005
The formation of DNA loops by proteins and protein complexes that bind at distal DNA sites plays a central role in many cellular processes, such as transcription, recombination, and replication. Here we review the basic thermodynamic concepts underlying the assembly of macromolecular complexes on looped DNA and the effects that this process has in the ...
arxiv   +1 more source

Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris

open access: yesVacciMonitor, 2021
The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors.
Sobirdjan A. Sasmakov   +7 more
doaj  

Physics of RecA-mediated homologous recognition [PDF]

open access: yes, 2004
Most proteins involved in processing DNA accomplish their activities as a monomer or as a component of a multimer containing a relatively small number of other elements. They generally act locally, binding to one or a few small regions of the DNA substrate. Striking exceptions are the \textit{E.
arxiv   +1 more source

Making tau amyloid models in vitro: a crucial and underestimated challenge

open access: yesFEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

Construction of Recombinant Vaccinia Viruses Using PUV-Inactivated Virus as a Helper

open access: yesBioTechniques, 2001
Recombinant vaccinia viruses (VVs) are widely used as expression vectors in molecular biology and immunology and are now under evaluation for gene therapy.
Tatyana M. Timiryasova   +3 more
doaj   +1 more source

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