Results 21 to 30 of about 551,717 (313)
PROTEIN BIOTECHNOLOGY: THE SIGNIFICANCE AND USE OF INDUSTRIAL POLYMER-DEGRADING ENZYMES [PDF]
Microorganisms are the primary source of industrial enzymes, with Bacillus and Aspergillus species being the leading producers. Bacillus strains have historically been used to synthesize commercially significant enzymes due to their status as GRAS ...
Mustafa Omar
doaj +1 more source
DNA looping: the consequences and its control [PDF]
The formation of DNA loops by proteins and protein complexes is ubiquitous to many fundamental cellular processes, including transcription, recombination, and replication. Here we review recent advances in understanding the properties of DNA looping in its natural context and how they propagate to the cellular behavior through gene regulation.
arxiv +1 more source
Investigating BB0405 as a novel Borrelia afzelii vaccination candidate in Lyme borreliosis
BB0405 is a surface exposed Borrelia burgdorferi protein and its vaccination protected mice against B. burgdorferi infection. As BB0405 is highly conserved across different B.
M. J. Klouwens+8 more
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Complementary Theory of Evolutionary Genetics [PDF]
This theory seeks to define species and to explore evolutionary forces and genetic elements in speciation and species maintenance. The theory explains how speciation and species maintenance are caused by natural selection acting on non-Mendelian and Mendelian variation, respectively. The emergence and maintenance of species as groups of populations are
arxiv
Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate
Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the research was to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinant technology.
Erma Sulistyaningsih+3 more
doaj +1 more source
DNA looping in gene regulation: from the assembly of macromolecular complexes to the control of transcriptional noise [PDF]
The formation of DNA loops by proteins and protein complexes that bind at distal DNA sites plays a central role in many cellular processes, such as transcription, recombination, and replication. Here we review the basic thermodynamic concepts underlying the assembly of macromolecular complexes on looped DNA and the effects that this process has in the ...
arxiv +1 more source
The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors.
Sobirdjan A. Sasmakov+7 more
doaj
Physics of RecA-mediated homologous recognition [PDF]
Most proteins involved in processing DNA accomplish their activities as a monomer or as a component of a multimer containing a relatively small number of other elements. They generally act locally, binding to one or a few small regions of the DNA substrate. Striking exceptions are the \textit{E.
arxiv +1 more source
Making tau amyloid models in vitro: a crucial and underestimated challenge
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley +1 more source
Construction of Recombinant Vaccinia Viruses Using PUV-Inactivated Virus as a Helper
Recombinant vaccinia viruses (VVs) are widely used as expression vectors in molecular biology and immunology and are now under evaluation for gene therapy.
Tatyana M. Timiryasova+3 more
doaj +1 more source