Complementary Theory of Evolutionary Genetics [PDF]
arXiv, 2021 This theory seeks to define species and to explore evolutionary forces and genetic elements in speciation and species maintenance. The theory explains how speciation and species maintenance are caused by natural selection acting on non-Mendelian and Mendelian variation, respectively. The emergence and maintenance of species as groups of populations are
arxiv
MRE11 facilitates the removal of human topoisomerase II complexes from genomic DNA [PDF]
, 2012 Topoisomerase II creates a double-strand break intermediate with topoisomerase covalently coupled to the DNA via a 5'-phosphotyrosyl bond. These intermediate complexes can become cytotoxic protein-DNA adducts and DSB repair at these lesions requires ...
Austin, Caroline +10 more
core +3 more sources
DNA induces conformational changes in a recombinant human minichromosome maintenance complex [PDF]
, 2015 ATP-dependent DNA unwinding activity has been demonstrated for recombinant archaeal homohexameric minichromosome maintenance (MCM) complexes and their yeast heterohexameric counterparts, but in higher eukaryotes such as Drosophila, MCM-associated DNA ...
Chaban, Y. +6 more
core +1 more source
DNA looping in gene regulation: from the assembly of macromolecular complexes to the control of transcriptional noise [PDF]
Current Opinion in Genetics & Development, 15, 136-144 (2005), 2005 The formation of DNA loops by proteins and protein complexes that bind at distal DNA sites plays a central role in many cellular processes, such as transcription, recombination, and replication. Here we review the basic thermodynamic concepts underlying the assembly of macromolecular complexes on looped DNA and the effects that this process has in the ...
arxiv +1 more source
VacciMonitor, 2021 The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors.
Sobirdjan A. Sasmakov +7 more
doaj
Physics of RecA-mediated homologous recognition [PDF]
, 2004 Most proteins involved in processing DNA accomplish their activities as a monomer or as a component of a multimer containing a relatively small number of other elements. They generally act locally, binding to one or a few small regions of the DNA substrate. Striking exceptions are the \textit{E.
arxiv +1 more source
Cloning and expression of the Propionibacterium shermanii methylmalonyl-CoA epimerase gene in Escherichia coli : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]
, 1994 Genomic DNA was isolated from Propionibacterium shermanii (52W). A 454 bp DNA fragment coding for the methylmalonyl-CoA epimerase (EC 5.1.99.1, subsequently referred to as epimerase) was amplified from genomic DNA by the polymerase chain reaction using ...
Saafi, 'Etuate Lui
core
Holliday junction resolvase in Schizosaccharomyces pombe has identical endonuclease activity to the CCE1 homologue YDC2 [PDF]
, 1998 A novel Holliday junction resolving activity has been identified in fractionated cell extracts of the fission yeast Schizosaccharomyces pombe . The enzyme catalyses endonucleolytic cleavage of Holliday junction-containing chi DNA and synthetic four-way ...
Keeley, A, Oram, M, Tsaneva, IR
core +2 more sources
FEBS Letters, EarlyView.We present the first solution structure of the Ca2+‐depleted LETM1 F‐EF‐hand through a D676A/N678A Ca2+ binding‐deficient mutant, revealing a closed hydrophobic cleft caused by a unique F1‐helix pivot. The apo LETM1 F‐EF‐hand exhibits regiospecific hot and cold unfolding, sensitivity to physiological pH changes and potential for promiscuous heterotypic
Qi‐Tong Lin +2 more
wiley +1 more source
Construction of Recombinant Vaccinia Viruses Using PUV-Inactivated Virus as a Helper
BioTechniques, 2001 Recombinant vaccinia viruses (VVs) are widely used as expression vectors in molecular biology and immunology and are now under evaluation for gene therapy.
Tatyana M. Timiryasova +3 more
doaj +1 more source