Results 151 to 160 of about 6,607,627 (360)

High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli

Microbial Cell Factories, 2013
BackgroundDisulfide-rich proteins or DRPs are versatile bioactive compounds that encompass a wide variety of pharmacological, therapeutic, and/or biotechnological applications.
Hervé Nozach, C. Fruchart‐Gaillard, F. Fenaille, F. Beau, O. Ramos, B. Douzi, N. Saez, M. Moutiez, D. Servent, M. Gondry, R. Thaï, P. Cuniasse, R. Vincentelli, V. Dive   +13 more
semanticscholar   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Factors influencing in vivo transduction by recombinant adeno-associated viral vectors expressing the human factor IX cDNA.

, 2001
Long-term expression of coagulation factor IX (FIX) has been observed in murine and canine models following administration of recombinant adeno-associated viral (rAAV) vectors into either the portal vein or muscle. These studies were designed to evaluate
Hanawa, H, Vanin, EF, Nathwani, AC, Zhou, JF, Davidoff, A, Nienhuis, AW   +5 more
core  

Interaction of Human Chorionic Gonadotropin (hCG) and Asialo-hCG with Recombinant Human Thyrotropin Receptor. [PDF]

, 1994
hCG is a putative thyroid stimulator. The present studies were undertaken to examine its interaction and that of its desialylated variant asialo-hCG with recombinant human TSH (hTSH) receptor (hTSHr). To this end, we transfected a human thyroid carcinoma
Mann, K., Grossmann, M., Derwahl, M., Hoermann, R., Broecker, M.   +4 more
core   +1 more source

Production of high-affinity glycosylated anti-mouse conjugated nanobodies in Pichia pastoris

Frontiers in Bioengineering and Biotechnology
IntroductionNanobodies (NBs) are small antibody fragments derived from camelid heavy-chain antibodies, which represent the minimal functional domain capable of antigen recognition and binding.
Sofía Orioli, Javier Santos, Javier Santos, Javier Santos, Lorena I. Ibañez, Lorena I. Ibañez, Cecilia D’Alessio, Cecilia D’Alessio   +7 more
doaj   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12

, 2007
NAD+-dependent malic enzyme (NAD-ME) gene from Escherichia coli K12 was inserted into an expression vector pET24b(+) and transformed into E coli BL21 (DE3).
Wang, Jinxia, Tan, Haidong, Zhao, Zongbao (Kent)   +2 more
core   +1 more source

Multiple Strategies for Recombinant Expression of Human Chymotrypsinogen C in Pichia Pastoris

Food Bioengineering
Chymotrypsin C (CTRC) is a multifunctional serine protease that have shown great application potential in the field of food processing. This study aims to efficiently express CTRC in Pichia pastoris and provide a safe and highly effective new enzyme ...
Yi Liu, Yu Xie, Xingyu Shen, Shihui Wang, Yi Xiong, Guimin Zhang   +5 more
doaj   +1 more source

Preparation of Cyclic Lunasin by Heterologous Recombination Combined with Enzymatic Cyclization and Its Properties

Shipin gongye ke-ji
In order to prepare a cyclic polypeptide cLunasin by the method of total biosynthesis, the linear precursor aLunasin was designed for cLunasin synthesis, and a fusion expression vector with the double tags of MBP and SUMO was constructed.
Fan SHI, Yi HOU, Jinsong ZHAO, Songqing HU   +3 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source