Results 141 to 150 of about 6,607,627 (360)
Molecules, 2017 Antimicrobial peptides represent an emerging category of therapeutic agents with remarkable structural and functional diversity. Modified vasoactive intestinal peptide (VIP) (VIP analogue 8 with amino acid sequence “FTANYTRLRRQLAVRRYLAAILGRR”) without ...
Chunlan Xu +5 more
doaj +1 more source
Tuning recombinant protein expression to match secretion capacity
Microbial Cell Factories, 2018 BackgroundThe secretion of recombinant disulfide-bond containing proteins into the periplasm of Gram-negative bacterial hosts, such as E. coli, has many advantages that can facilitate product isolation, quality and activity.
Luminita Gabriela Horga +10 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
CropsDrastic changes in temperature, salinity of soils and drought are some of the most studied abiotic stressors in important crops. Plants have developed various biochemical mechanisms to counteract these conditions. Transcription factors play a significant
Diobel González-Stewart +8 more
doaj +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Recombinant gelatin and collagen from methylotrophic yeasts
, 2002 Based on its structural role and compatibility within the human body, collagen is a commonly used biomaterial in medical applications, such as cosmetic surgery, wound treatment and tissue engineering.
Bruin, E.C., de
core
BioTechHuman embryonic kidney (HEK293) cells are a widespread choice for recombinant protein expression. To optimise yields, the hydrolysate Tryptone N1 (TN1) is commonly added post-transfection. TN1 is obtained by controlled enzymatic digestion of casein.
Shafqat Shabir +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia +3 more
wiley +1 more source
Introduction of recombinant antigens against Jembrana Disease Virus into plants
, 2010 Jembrana disease virus (JDV) is a bovine lentivirus that causes an acute severe disease syndrome in banteng cattle (Bos javanicus) in Indonesia. In order to develop a vaccine, JDV capsid (CA) protein together with transactivator of transcription (Tat ...
Singh, Meenu
core
Agriculture, 2012 Botrytis cinerea is a necrotrophic fungus infecting over 230 plant species worldwide. This highly adaptable pathogen can afflict agricultural products from seed to storage, causing significant economic losses and instability in the food supply.
Maria R. Davis +3 more
doaj +1 more source