Intrinsic-Extrinsic Convolution and Pooling for Learning on 3D Protein Structures [PDF]
arXiv, 2020 Proteins perform a large variety of functions in living organisms, thus playing a key role in biology. As of now, available learning algorithms to process protein data do not consider several particularities of such data and/or do not scale well for large protein conformations.
arxiv
Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells
Microbial Cell Factories, 2005 Background Native as well as recombinant bacterial cell surface layer (S-layer) protein of Geobacillus (G.) stearothermophilus ATCC 12980 assembles to supramolecular structures with an oblique symmetry. Upon expression in E.
Veenhuis Marten +4 more
doaj +1 more source
Advanced Science, EarlyView.Based on a well‐established in vitro directed differentiation model and an integrated analysis of high‐density cell lineage trees (CLTs) and single‐cell transcriptomes, it is demonstrated that many subclones are formed by sub‐CLTs resembling each other in terms of both cell type compositions and topological structures.
Xiaoyu Zhang +14 more
wiley +1 more source
Expression of an active form of recombinant Ty1 reverse transcriptase in Escherichia coli: a fusion protein containing the C-terminal region of the Ty1 integrase linked to the reverse transcriptase–RNase H domain exhibits polymerase and RNase H activities [PDF]
, 2000 M.L. Wilhelm +2 more
openalex +2 more sources
Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites. [PDF]
, 1990 David L. Rimm +5 more
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Bilirubin Targeting WNK1 to Alleviate NLRP3‐Mediated Neuroinflammation
Advanced Science, EarlyView.At physiological concentrations, bilirubin binds to the kinase domain of WNK1, thereby augmenting its activity and facilitating the phosphorylation of downstream SPAK/OSR1. This phosphorylation inhibits KCC2 activity, leading to elevate intracellular chloride levels in neurons.
Linfei Mao +14 more
wiley +1 more source
Modulation of N- to C-terminal interactions enhances protein stability [PDF]
arXiv, 2015 Although, several factors have been attributed to thermostability, the stabilization strategies used by proteins are still enigmatic. Studies on recombinant xylanase which has the ubiquitous (\b{eta}/{\alpha})8 TIM (Triosephosphate isomerase) barrel fold showed that, just a single extreme N-terminus mutation (V1L) markedly enhanced the thermostability ...
arxiv
Brazilian Journal of Medical and Biological Research, 2004 We report here the construction of a vector derived from pET3-His and pRSET plasmids for the expression and purification of recombinant proteins in Escherichia coli based on T7 phage RNA polymerase.
C.R.R. Ramos +3 more
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Phosphorylation of recombinant human phenylalanine hydroxylase: effect on catalytic activity, substrate activation and protection against non-specific cleavage of the fusion protein by restriction protease [PDF]
, 1996 Anne P. Døskeland +3 more
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Advanced Science, EarlyView.Hepatoblastoma, an aggressive childhood liver tumor, origins from malignant hepatoblast‐like cells. Single‐cell analysis reveals IGF2‐driven pathways promoting malignancy. Targeting these pathways offers promising treatments, with serum IGF2 as a potential diagnostic biomarker.
Miao Ding +12 more
wiley +1 more source