Results 91 to 100 of about 262,160 (309)

Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells [PDF]

, 2007
Background Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components.
Buriani, Giampaolo, Nuttall, James, Marusic Carla, Lombardi Raffaele, Lombardi, Raffaele, Baschieri , Selene, Buriani Giampaolo, Eugenio Benvenuto, Lico Chiara, Baschieri Selene, Frigerio, Lorenzo, James Nuttall, Benvenuto Eugenio, Lorenzo Frigerio, Nuttall James, Giampaolo Buriani, Marusic, Carla, Selene Baschieri, Frigerio Lorenzo, Raffaele Lombardi, Benvenuto, Eugenio, Chiara Lico, Carla Marusic, Lico, Chiara   +23 more
core   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Turn TRAIL Into Better Anticancer Therapeutic Through TRAIL Fusion Proteins

Cancer Medicine
Background TNF‐related apoptosis‐inducing ligand (TRAIL) belongs to the tumor necrosis factor superfamily. TRAIL selectively induces apoptosis in tumor cells while sparing normal cells, which makes it an attractive candidate for cancer therapy ...
Yan Wang, Xin Qian, Yubo Wang, Caiyuan Yu, Li Feng, Xiaoyan Zheng, Yaya Wang, Qiuhong Gong   +7 more
doaj   +1 more source

Microfabricated Anisotropic Myobundles for the Scalable Production of Cardiac Tissue Grafts

Advanced Functional Materials, EarlyView.
Controlling the anisotropy of cardiac tissue remains an outstanding challenge in the field of cardiac tissue engineering. Here, we introduce an approach to generate anisotropic cardiac myobundles using cell‐adhesive, synthetic, electrospun fibers and stem cell‐derived cardiac fibroblasts.
Maggie E. Jewett, Javiera Jilberto, Margaret E. Stanley, David O. Ntim, Siddhi M. Bhirud, Avianna T. Schroeder, Gonzola X. Anyosa Galvez, Jingyi Xia, Samuel J. DePalma, Emmanouil Agrafiotis, Susan S. Xi, Amanda S. Bluem, Courtney M. Lestock, Adam S. Helms, David A. Nordsletten, Brendon M. Baker   +15 more
wiley   +1 more source

Topological Scaffold‐Based Trispecific Recombinant Protein‐Drug Conjugates for Solid Tumor Eradication

Advanced Science
Topology structure of proteins plays vital roles on their bioactivity and property. However, the understanding of how the biological effect of active targeting groups varies with different spatial positions in the recombinant protein scaffold is poor ...
Huiyi Jiang, Yang Yuan, Xiaoke Zhang, Weizhi Chen, Hui Mao, Baorui Liu, Xiqun Jiang   +6 more
doaj   +1 more source

Bacterial‐Electrochemical Platform Utilizing a MXene‐Peptide Hydrogel

Advanced Functional Materials, EarlyView.
A peptide‐based fibrillar hydrogel incorporating MXene facilitates efficient electron delivery to intracellular recombinant [FeFe]‐hydrogenase enzyme in E. coli, enabling sustained bioelectrochemical H2 production without engineered exoelectrogenicity pathways.
Oren Ben‐Zvi, Itzhak Grinberg, Or Messer, Dana Cohen‐Gerassi, Maxim Sokol, Iftach Yacoby, Lihi Adler‐Abramovich   +6 more
wiley   +1 more source

Improved methods for production and characterisation of Jembrana disease virus proteins

, 2010
Jembrana disease is an acute disease of Bali cattle (Bos javanicus) in Indonesia caused by Jembrana disease virus (JDV), a lentivirus most closely related to Bovine immunodeficiency virus. Control of the disease in Bali cattle, which are important to the
Rachmat, Judhi
core  

Catcher Domains as Multifunctional Fusion Modules for Soluble Expression, Covalent Coupling, and Spatial Organization of Recombinant Proteins

Small Science
Fusion tags are essential tools for recombinant protein production, but their functions are often limited to improving solubility or facilitating purification.
Ruxia Fan, Xing Wan, Safoorah Khanum, Juhani P. Jokio, Nea B. Möttönen, Miia R. Mäkelä, A. Sesilja Aranko   +6 more
doaj   +1 more source

Aqueous Two‐Phase Bioinks for Discrete Packing and Compartmentalization of 3D Bioprinted Cells

Advanced Healthcare Materials, EarlyView.
Aqueous two‐phase systems (ATPS) enable the formation of biomimetic interfaces crucial for tissue engineering. However, clinical translation remains limited by the challenge of precisely controlling cellular compartmentalization. Here, we developed ATPS biomaterial inks for 3D bioprinting allowing tuneable droplet formation via NaCl modulation.
Martina Marcotulli, Arianna Iacomino, Federico Serpe, Lucia Iafrate, Marco Bastioli, Giorgia Montalbano, Michele D'Orazio, Biagio Palmisano, Silvia Franco, Arianna Mencattini, Roberta Angelini, Eugenio Martinelli, Alessandro Corsi, Mara Riminucci, Giancarlo Ruocco, Chiara Scognamiglio, Andrea Barbetta, Gianluca Cidonio   +17 more
wiley   +1 more source

Chromatographic Analysis of Recombinant Lysostaphin Expressed in Escherichia coli

, 2011
Lysostaphin (EC.3.4.24.75) is an extracellular glycylglycine endopeptidase produced exclusively by Staphylococcus simulans biovar staphylolyticus (ATCC 1362, NRRL B-2628).
Jennings, Claire
core