Results 111 to 120 of about 262,160 (309)

Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells [PDF]

, 2011
Background: Chikungunya virus (CHIKV) is a mosquito-borne, arthrogenic Alphavirus that causes large epidemics in Africa, South-East Asia and India. Recently, CHIKV has been transmitted to humans in Southern Europe by invading and now established Asian ...
Geertsema, C (Corinne), Corinne Geertsema, van Oers, MM, Paulina Andrade, Heldens, J.G.M., Geertsema, C., Vlak Just M, Pijlman Gorben P, Andrade, Paulina, Vlak, J.M. (Just), Vlak, J.M., Pijlman, G.P. (Gorben), Just M Vlak, Metz Stefan W, Goldbach, RW, Metz, S.W. (Stefan), Martina, B.E.E. (Byron), Geertsema Corinne, Metz, S.W.H., Stefan W Metz, Jacco G Heldens, Geertsema, C. (Corinne), Heldens Jacco G, Martina, Byron, Andrade, P. (Paulina), Metz, SW, Andrade Paulina, Heldens, J., Rob W Goldbach, Pijlman, G.P., Pijlman, GP, van Oers Monique M, Oers, M.M. (Monique) van, van Oers, M.M., Andrade, P, Goldbach, R.W., Gorben P Pijlman, Monique M van Oers, Vlak, JM, Goldbach Rob W, Heldens, JG, Byron E Martina, Martina, Byron E., Martina Byron E, Oers, M.M., van   +44 more
core   +1 more source

Gut Microbiota Regulates Systemic Inflammatory Response and Compensatory Anti‐Inflammatory Response Syndromes by Targeting PF4+ Macrophages in Acute Pancreatitis

Advanced Science, EarlyView.
ABSTRACT Acute pancreatitis (AP) begins with pancreatic local inflammation, leading to the onset of systemic inflammatory response syndrome (SIRS), followed by compensatory anti‐inflammatory response syndrome (CARS), which causes immune paralysis and higher mortality rate.
Liwei Liu, Guanqun Li, Dongxu Lu, Haoran Ding, Tianqi Lu, Yuhang Sui, Can Zhang, Yu Xie, Rui Kong, Hua Chen, Xuewei Bai, Hongtao Tan, Dongbo Xue, Xianzhi Meng, Le Li, Bei Sun   +15 more
wiley   +1 more source

Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein

Journal of Nanobiotechnology, 2009
Ultrashort pulsed laser ablation in liquids represents a powerful tool for the generation of pure gold nanoparticles (AuNPs) avoiding chemical precursors and thereby making them especially interesting for biomedical applications.
Bullerdiek Jörn, Richter Andreas, Wagner Siegfried, Soller Jan T, Petersen Svea, Nolte Ingo, Barcikowski Stephan, Escobar Hugo   +7 more
doaj   +1 more source

ERK‐Mediated Phosphorylation of YAP Defines a Noncanonical FGF Signaling Mechanism in Stem Cells

Advanced Science, EarlyView.
With a powerful combination of in vivo, ex vivo, and in vitro models, the authors highlight a novel FGF‐ERK signaling regulation of YAP at the S128 site in neural crest‐derived stem cells. This study opens exciting new directions in stem cell biology and craniofacial biology, paving the way for potential innovations in the treatment of craniofacial ...
Xiaolei Zhao, Shannon Erhardt, Li Tang, Xiaotong Chen, Stephen M Farmer, Zixiu Cheng, Wen Chen, Ella Ziyuan Lu, Kihan Sung, Chang‐Ru Tsai, Mingjie Zheng, Sheng Zhang, Yang Liu, Jianxin Wang, Min Li, James F Martin, Jun Wang   +16 more
wiley   +1 more source

Optimisation of the production of cathepsin L1 from a recombinant saccharomyces cerevisiae [PDF]

, 2002
Cathepsin L1 is a cysteine protease that has been previously isolated and functionally expressed in Saccharomyces cerevisiae. It has the potential to be employed as a vaccine for liver-fluke disease in cattle and other ruminants.
O'Donovan, Eimear C
core  

An albumin-derived peptide scaffold capable of binding and catalysis

, 2012
We have identified a 101-amino-acid polypeptide derived from the sequence surrounding the IIA binding site of human albumin. The polypeptide contains residues that make contact with ligands as warfarin in the parent protein, and eight cysteine residues ...
SBLATTERO, DANIELE, Fabio Benedetti, Giampaolo Fontanive (381542), SGARRA, RICCARDO, Daniele Sblattero (251886), Riccardo Sgarra (103906), Immacolata Luisi (381540), MAURIZIO, ELISA, Silvia Pavan (381541), Fabio Benedetti (243799), BENEDETTI, FABIO, Adriano Savoini, LUISI, Immacolata, Alessandro Tossi, Federico Berti, Immacolata Luisi, FONTANIVE, GIAMPAOLO, Silvia Pavan, Federico Berti (381545), BERTI, FEDERICO, Alessandro Tossi (381543), Elisa Maurizio, Ro Tossi, Adriano Savoini (381544), TOSSI, ALESSANDRO, Daniele Sblattero, Savoini A., PAVAN, SILVIA, Riccardo Sgarra, Elisa Maurizio (103901), Giampaolo Fontanive   +30 more
core   +1 more source

Marburg Virus Glycoprotein Is a Remarkable Virulent Factor Linked to Hemorrhagic Pathology: Evidence from Multimodal Experimental Systems

Advanced Science, EarlyView.
By integrating data from in vitro, ex vivo, and in vivo models, our research identifies the MARV glycoprotein as a remarkable hemorrhagic factor, filling a major gap in this important field. It also provides practical experimental tools for the basic research on viral pathogenesis and applied research aimed at antiviral intervention for hemorrhagic ...
Ting Yao, Hang Liu, Yanfeng Yao, Wei Deng, Jiawen Sun, Zhenyu Kang, Ashaq Ali, Chao Shan, Zhiming Yuan, Fei Deng, Hualin Wang, Yun‐Jia Ning   +11 more
wiley   +1 more source

LC3B Mediated SETDB1‐Accounted Alcoholic Steatohepatitis via Lipidation‐Dependent LAP and Lipidation‐Independent Nuclear Stabilization

Advanced Science, EarlyView.
SETDB1 is progressively downregulated in ALD, correlating with disease severity. SETDB1 deficiency impairs LAP by disrupting Rubicon membrane localization, leading to defective lipid droplet clearance. Concurrently, loss of SETDB1 reduces nuclear LC3B, causing R‐loop accumulation and cGAS‐STING‐driven inflammation. Lipidated LC3B mediates LAP‐dependent
Yi Zhang, Tan Wei, Jiahang Wu, Chuixu Lin, Dongbo Zhu, Yanhui Li, Shuting Shi, Shishun Huang, Leiming Jiang, Hongzhi Wang, Meiqi Song, Pengfei Gao, Xu Wu, Mingjian Fan, Chaofeng Wei, Qian Wang, Lihui Qu, Zhigang Wang   +17 more
wiley   +1 more source

Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl‐tRNA Synthetase Boosts Genetic Code Expansion in E. coli

Advanced Science, EarlyView.
ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Nikolaj G. Koch, Peter Goettig, Michael A. Nash, Juri Rappsilber, Nediljko Budisa   +4 more
wiley   +1 more source

An efficient genome-wide fusion partner screening system for secretion of recombinant proteins in yeast

, 2015
To produce rarely secreted recombinant proteins in the yeast Saccharomyces cerevisiae, we developed a novel genome-wide optimal translational fusion partner (TFP) screening system that involves recruitment of an optimal secretion signal and fusion ...
Jung Hoon Bae, Jung Hoon Sohn, Soon-Ho Park, Kwang Mook Lim, Bong Hyun Sung, Hyun-Jin Kim, Mi Jin Kim, Cho-Ryong Lee   +7 more
core   +1 more source