Results 71 to 80 of about 282,766 (336)
Develop machine learning based predictive models for engineering protein solubility [PDF]
arXiv, 2018 Protein activity is a significant characteristic for recombinant proteins which can be used as biocatalysts. High activity of proteins reduces the cost of biocatalysts. A model that can predict protein activity from amino acid sequence is highly desired, as it aids experimental improvement of proteins.
arxiv
Hematopoietic cancers and Nup98 fusions: determining common mechanisms of malignancy [PDF]
, 2012 Chromosomal aberrations are very frequent in leukemias and several recurring mutations capable of malignant transformation have been described. These mutations usually occur in hematopoietic stem cells (HSC), transforming them into leukemia stem cells.
arxiv +1 more source
FimH-based display of functional eukaryotic proteins on bacteria surfaces
Scientific Reports, 2019 The demand for recombinant proteins for analytic and therapeutic purposes is increasing; however, most currently used bacterial production systems accumulate the recombinant proteins in the intracellular space, which requires denaturating procedures for ...
Markus Chmielewski +5 more
doaj +1 more source
Human αB-crystallin as fusion protein and molecular chaperone increases the expression and folding efficiency of recombinant insulin. [PDF]
PLoS ONE, 2018 Low expression and instability are significant challenges in the recombinant production of therapeutic peptides. The current study introduces a novel expression and purification system for human insulin production using the molecular chaperone αB ...
Mohsen Akbarian, Reza Yousefi
doaj +1 more source
Advanced Functional Materials, EarlyView.A series of ultraviolet‐ and near‐infrared radiation sensitive photoactivatable hydrogels are developed that can be controlled spatiotemporally down to µm precisions using one‐ and two‐photon uncaging. The latent hydroxylamine‐, hydrazine‐, and amine uncaged moieties are used to photopattern a series of diverse proteins displaying complimentary ...
Tove Kivijärvi +6 more
wiley +1 more source
Utilizing of protein splicing phenomenon for optimization of obtaining and purification of the recombinant human growth hormone [PDF]
Europ J Medic Res., 2006, Oct. 8, vol.11 - p. 128-129, 2006 Protein splicing is a post-translational autocatalystic excision of internal protein sequence (intein) with the subsequent ligation of the flanking polypeptides (exteins). The high specificity of excision ensured by intein makes it possible to use a phenomenon of protein splicing for the biotechnology purposes.
arxiv
Design and expression of TolC as a recombinant protein vaccine against Shigella flexneri and evaluation of immunogenic response in mice [PDF]
arXiv, 2023 Background: Shigella is one of the major cause of dysenteric diarrhea, which is known shigelosis. Shigelosis causes 160,000 deaths annually of diarrheal disease in a global scale especially children less than 5 years old. No licensed vaccine is available against shigelosis, therefore, efforts for develop an effective and safe vaccine against shigella ...
arxiv
Advanced Functional Materials, EarlyView.Designer DNA Architecture‐templated Drug Conjugates (DDA‐DCs) are comprised of a DNA nanomaterial decorated with multiple drug‐loaded DNA aptamers. DDA‐DCs use a combination of multiple aptamer types, each recognizing a different membrane protein to achieve precise targeting of cancer cells.
Abhisek Dwivedy +19 more
wiley +1 more source
Advanced Functional Materials, EarlyView.This review highlights recent advances in engineering artificial antigen‐presenting cells (aAPCs) as alternatives to dendritic cells for T cell expansion. Key design principles inspired by the immunological synapse are discussed, with emphasis on strategies for polyclonal and antigen‐specific T cell expansion.
Nguyen Thi Nguyen, Yu Seok Youn
wiley +1 more source
FIT: Tag based method for fusion proteins identification [PDF]
arXiv, 2014 There is increased interest in the identification and analysis of gene fusions and chimeric RNA transcripts. While most recent efforts focused on the analysis of genomic and transcriptomic data, identi-fication of novel peptides corresponding to such events in mass spectrometry-based proteomic datasets would provide complemen-tary, protein-level ...
arxiv