Results 11 to 20 of about 171,370 (219)

Recent Progress on the Versatility of Virus-Like Particles

Vaccines, 2020
Virus-like particles (VLPs) are multimeric nanostructures composed of one or more structural proteins of a virus in the absence of genetic material. Having similar morphology to natural viruses but lacking any pathogenicity or infectivity, VLPs have ...
Ciying Qian, Xinlin Liu, Qin Xu, Zhiping Wang, Jie Chen, Tingting Li, Qingbing Zheng, Hai Yu, Ying Gu, Shaowei Li, Ningshao Xia   +10 more
doaj   +1 more source

Bacterially produced recombinant influenza vaccines based on virus-like particles. [PDF]

PLoS ONE, 2013
Although current influenza vaccines are effective in general, there is an urgent need for the development of new technologies to improve vaccine production timelines, capacities and immunogenicity.
Andrea Jegerlehner, Franziska Zabel, Alice Langer, Klaus Dietmeier, Gary T Jennings, Philippe Saudan, Martin F Bachmann   +6 more
doaj   +1 more source

Plasmid DNA-based Alphavirus Vaccines

Vaccines, 2019
Alphaviruses have been engineered as vectors for high-level transgene expression. Originally, alphavirus-based vectors were applied as recombinant replication-deficient particles, subjected to expression studies in mammalian and non-mammalian cell lines,
Kenneth Lundstrom
doaj   +1 more source

Papillomavirus Infectious Pathways: A Comparison of Systems

Viruses, 2015
The HPV viral lifecycle is tightly linked to the host cell differentiation, causing difficulty in growing virions in culture. A system that bypasses the need for differentiating epithelium has allowed for generation of recombinant particles, such as ...
Jennifer Biryukov, Craig Meyers
doaj   +1 more source

ACE2-cytomimetic particles restrict SARS-Cov-2 spike protein binding to cellular targets

Biotechnology Reports, 2021
The development of countermeasures that aid in the prevention and propagation of SARS-CoV-2 infections is critical to manage the continuing crisis brought about by COVID-19.
Thomas A. Strong, Daniel Pelaez
doaj   +1 more source

Inhibition of lecithin:cholesterol acyltransferase activity by synthetic phosphatidylcholine species containing eicosapentaenoic acid or docosahexaenoic acid in the sn-2 position.

Journal of Lipid Research, 1992
Phospholipids isolated from the plasma of monkeys fed a diet enriched in fish oil were poor substrates for cholesteryl ester (CE) synthesis by the lecithin:cholesterol acyltransferase (LCAT) reaction relative to those from animals fed a lard containing ...
JS Parks, TY Thuren, JD Schmitt
doaj   +1 more source

Production in yeast of pseudotype virus-like particles harboring functionally active antibody fragments neutralizing the cytolytic activity of vaginolysin

Microbial Cell Factories, 2011
Background Recombinant antibodies can be produced in different formats and different expression systems. Single chain variable fragments (scFvs) represent an attractive alternative to full-length antibodies and they can be easily produced in bacteria or ...
Pleckaityte Milda, Zvirbliene Aurelija, Sezaite Indre, Gedvilaite Alma   +3 more
doaj   +1 more source

Isolation of Particles of Recombinant ASC and NLRP3

Bio-Protocol, 2015
NLRP3 inflammasome is a multiprotein complex responsible for the activation of inflammatory caspase-1, resulting in processing and release of pro-inflammatory cytoquines IL-1β and IL-18 (Schroder and Tschopp, 2010).
Fátima Martín-Sánchez, Ana Gómez, Pablo Pelegrín   +2 more
doaj   +1 more source

Immunogenic and Protective Features of the Recombinant Vaccinia Virus Strain Expressing Cassette of Genes of Marburg Virus Structural Proteins

Проблемы особо опасных инфекций, 2022
The aim of the study was to create a highly immunogenic vaccine construct based on a recombinant variant of a replication-defective MVA strain of vaccinia virus, expressing virus-like particles that mimic natural infection with Marburg virus.
A. V. Semenova, G. F. Sivolobova, A. A. Grazhdantseva, S. A. P’yankov, I. S. Shul’gina, O. S. Taranov, O. V. P’yankov, D. V. Antonets, L. I. Karpenko, E. V. Starostina, M. V. Borgoyakova, A. N. Chikaev, E. V. Gavrilova, R. A. Maksyutov, G. V. Kochneva   +14 more
doaj   +1 more source

Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles

BioTech, 2023
Immobilized metal affinity chromatography (IMAC) is a popular and valuable method for the affinity purification of polyhistidine-tagged recombinant proteins.
Jule L. Völzke, Sarah Smatty, Sarah Döring, Shireen Ewald, Marcus Oelze, Franziska Fratzke, Sabine Flemig, Zoltán Konthur, Michael G. Weller   +8 more
doaj   +1 more source