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Recombinant Proteins in Vaccine Development
2003The outer membrane of Neisseria meningitidis contains a variety of proteins with the potential for inclusion in new meningococcal vaccines (1). Studies on the vaccine potential of these proteins would be facilitated by the production of pure recombinant protein, free from other components of the Neisseria outer membrane.
M, Christodoulides +2 more
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Preparation of recombinant neuritin protein
Protein Expression and PurificationNeuritin plays an important role in promoting nerve injury repair and maintaining synaptic plasticity, making it a potential therapeutic target for the treatment of nerve injury and neurodegenerative diseases. The present study aimed to obtain an active, unlabeled neuritin protein.
Pingping, Meng +6 more
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Recombinant protein production and streptomycetes
Journal of Biotechnology, 2012The biopharmaceutical market has come a long way since 1982, when the first biopharmaceutical product, recombinant human insulin, was launched. Just over 200 biopharma products have already gained approval. The global market for biopharmaceuticals which is currently valued at over US$99 billion has been growing at an impressive compound annual growth ...
Jozef, Anné +4 more
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Purification of BmR1 Recombinant Protein
The Protein Journal, 2010This paper describes a refinement in the purification step that facilitated the downstream recovery of high purity BmR1 recombinant protein, which is a protein used as a test reagent in the commercialized rapid tests for detection of lymphac filariasis i.e. Brugia Rapid™ and panLF rapid™.
Norsyahida, Arifin +4 more
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The expression of recombinant proteins in yeasts
Current Opinion in Biotechnology, 1996The methylotrophic yeasts Hansenula polymorpha and Pichia pastoris are rapidly becoming the systems of choice for the expression of recombinant proteins in yeast. However, the powerful genetic techniques available in Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe are still exploited to establish models to study medically ...
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Selenomethionine Labeling of Recombinant Proteins
2007Selenomethionine incorporation is a standard method for determining the phases in protein crystallography by single- or multiwavelength anomalous dispersion. Recombinant expression of selenomethionine-containing protein in non-auxotrophic Pichia pastoris strains yield an incorporation of about 50%.
Anna M, Larsson, T Alwyn, Jones
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Recombinant Protein Expression in Nicotiana
2010Recombinant protein pharmaceuticals are now widely used in treatment of chronic diseases, and several recombinant protein subunit vaccines are approved for human and veterinary use. With growing demand for complex protein pharmaceuticals, such as monoclonal antibodies, manufacturing capacity is becoming limited.
Nobuyuki, Matoba +2 more
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Challenges in production of recombinant proteins
Bioprocess and Biosystems Engineering, 2007The sequencing of the human genome and genomes from lower species has led molecular biology research into the era of proteomics. Starting from the cDNA’s, a large number of proteins have to be expressed in quantities large enough to allow determining of their structure and function, which forms the basis for the development of new pharmaceutical ...
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Industrial Challenges of Recombinant Proteins
2019The use of recombinant DNA methods to produce large quantities of protein for therapeutic uses has revolutionized medicine. Industrial challenges for manufacture of biotherapeutic proteins are related to the characteristics of these proteins and the increasing quantities required to address needs of patients, worldwide. A brief overview of therapies in
Scott R, Rudge, Michael R, Ladisch
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Heavy Labeling of Recombinant Proteins
2007Because of the cost of isotopic chemicals and heterologous proteins to produce, an economical 15N/13C isotopic labeling method is critically needed. Four protocols have been tested for the expression of Ovine interferon-tau in Pichia pastoris. 13C-glucose in place of 13C-glycerol as well as the need for 15N/13C-sources were evaluated during the growth ...
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