Results 261 to 270 of about 952,798 (315)
Some of the next articles are maybe not open access.
Recombinant Protein Production in Yeasts
2011Recombinant protein production is a multibillion-dollar market. The development of a new product begins with the choice of a production host. While one single perfect host for every protein does not exist, several expression systems ranging from bacterial hosts to mammalian cells have been established.
Mattanovich, D +5 more
openaire +3 more sources
Insights on the Formulation of Recombinant Proteins
2019Recombinant proteins are large and complex molecules, whose therapeutic activity highly depends on their structure. Formulation of biopharmaceuticals aims at stabilizing protein conformation, promoting its efficacy, and preventing safety concerns, such as immunogenicity. Currently, the rational design of formulations is possible due to the availability
Ribeiro, Rita +4 more
openaire +3 more sources
Overview of the Characterization of Recombinant Proteins
Current Protocols in Protein Science, 1995AbstractThis overview provides guidelines for the characterization of recombinantly expressed proteins (e.g., verifying primary structure and appropriate post‐translational modifications), along with methodologies for characterizing the proteins according to size, X‐ray structure, absorbance, biological activity, and subunit structure.
Denslow, Nancy D. +2 more
openaire +3 more sources
Recombinant proteins for genetic disease
Clinical Genetics, 1999The era of molecular biology has led to the development of powerful tools capable of generating therapeutics for genetic disorders. Although there is much current emphasis placed on the development of ‘gene therapy’ for human disease, developments in the production and availability of recombinant proteins are likely to have a more substantial impact on
C S, Russell, L A, Clarke
openaire +2 more sources
Recombinant Therapeutic Protein Vaccines
Protein & Peptide Letters, 2013Recombinant technology has ushered in a new era for the pharmaceutical industry. Protein therapeutics, including plasma-derived products and antibodies obtained from the serum of infected patients, have been successfully adopted and utilized to treat various indications.
Satoshi, Ohtake, Tsutomu, Arakawa
openaire +2 more sources
Overproduction of recombinant proteins in plants
Plant Science, 2012Recombinant protein production in microbial hosts and animal cell cultures has revolutionized the pharmaceutical and industrial enzyme industries. Plants as alternative hosts for the production of recombinant proteins are being actively pursued, taking advantage of their unique characteristics.
Erin, Egelkrout +2 more
openaire +2 more sources
Preparation of Recombinant Proteins in Milk
2004Using transgenic animals as the source of recombinant proteins has several specific advantages. Large amounts of proteins can be obtained, essentially from milk. These proteins are often properly processed. They are in a number of cases correctly folded, assembled, cleaved, glycosylated, gamma-carboxylated, and so on.
openaire +3 more sources
[Recombinant protein folding and production].
Medecine sciences : M/S, 2005The biotechnology of recombinant protein production is now entering its most advanced stage, and the growth of industrial protein pharmaceuticals provides solid proof of this evolution. However, the systematic conversion of genetic information into a biologically active protein is constantly confronted by the fundamental problem of protein folding in ...
Betton, Jean-Michel, Chaffotte, Alain
openaire +2 more sources
Capillary electrophoresis of recombinant proteins
Journal of Chromatography B: Biomedical Sciences and Applications, 1997Many naturally occurring proteins which are used therapeutically have been cloned and expressed in large quantities in bacterial, yeast or mammalian systems. Purification of these proteins by column chromatography generates high purity products with low levels of host protein contaminants.
K A, Denton, S A, Tate
openaire +2 more sources
SCHEMA-Guided Protein Recombination
2004Publisher Summary This chapter examines the different aspects of SCHEMA-guided protein recombination. SCHEMA is a scoring function that predicts which elements in homologous proteins can be swapped without disturbing the integrity of the structure. Using the structural coordinates of the parent proteins, SCHEMA identifies pairs of residues that are ...
Silberg, Jonathan J. +2 more
openaire +3 more sources