Results 121 to 130 of about 1,100,263 (385)
FEBS Open Bio, EarlyView.Etoposide, a topoisomerase II inhibitor, reduces O‐GlcNAcylation in HepG2 liver cancer cells. Further inhibition of O‐GlcNAc transferase by OSMI‐1 enhanced etoposide‐induced apoptosis, lowering the IC50 for viability and increasing the EC50 for cytotoxicity.
Jaehoon Lee +5 more
wiley +1 more source
The recombinase protein is a torque sensitive molecular switch [PDF]
arXiv, 2016 How a nano-searcher finds its nano-target is a general problem in non-equilibrium statistical physics. It becomes vital when the searcher is a damaged DNA fragment trying to find its counterpart on the intact homologous chromosome. If the two copies are paired, that intact homologous sequence serves as a template to reconstitute the damaged DNA ...
arxiv
Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]
, 1993 A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Amman +29 more
core +1 more source
FEBS Open Bio, EarlyView.ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu +6 more
wiley +1 more source
Ciz1 cooperates with cyclin-A-CDK2 to activate mammalian DNA replication in vitro [PDF]
, 2010 Initiation of mammalian DNA replication can be reconstituted from isolated G1-phase nuclei and cell extracts, supplemented with cyclin-dependent protein kinases (CDKs).
Ainscough +30 more
core +3 more sources
FEBS Open Bio, EarlyView.Stem cell‐based embryo models (SCBEMs) are valuable to study early developmental milestones. Matrigel, a basement membrane matrix, is a critical substrate used in various SCBEM protocols, but its role in driving stem cell lineage commitment is not clearly defined.
Atoosa Amel +3 more
wiley +1 more source
PRODUCTION OF HIGH LEVELS OF TRANSGENC FACTOR IX WITHOUT GENE RESCUE, AND ITS THERAPEUTIC USES [PDF]
, 2011 A non-human transgenic mammalian animal, as described above, contains an exogenous double stranded DNA sequence stably integrated into the genome of the animal, which comprises cis-acting regulatory units operably linked to a DNA sequence encoding human ...
Cooper, Julian D. +2 more
core +1 more source
Development of 4T1 breast cancer mouse model system for preclinical carbonic anhydrase IX studies
FEBS Open Bio, EarlyView.Carbonic anhydrase IX (CAIX) is a well‐recognised therapeutic target and prognostic biomarker in cancer. We developed and characterised a robust murine breast cancer model system that is suitable for CAIX studies in vitro and in vivo—it comprises both CAIX‐positive and CAIX‐negative controls and provides a solid platform for the comprehensive ...
Zane Kalniņa +13 more
wiley +1 more source
مجله دانشگاه علوم پزشکی بیرجند, 2018 Background and Aim: Short tandem repeat (STR) markers, are conserved region in human genome and highly polymorphic between individuals. Nowadays, genotyping of STR marker is widely known and used for the genetic identification of individuals in forensic ...
Mohammadreza Nassiri +6 more
doaj