Results 121 to 130 of about 6,420,190 (386)
FEBS Open Bio, EarlyView.Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley +1 more source
PRODUCTION OF RECOMBINANT PROTEINS IN INSECT CELLS
, 2013 Among the wide range of methods and expression hosts available for the heterologous production of recombinant proteins, insect cells are ideal for the production of complex proteins requiring extensive post-translational modification. This review article
Christian Kollewe, A. Vilcinskas
semanticscholar +1 more source
FEBS Open Bio, EarlyView.SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel +13 more
wiley +1 more source
مجله دانشگاه علوم پزشکی بیرجند, 2018 Background and Aim: Short tandem repeat (STR) markers, are conserved region in human genome and highly polymorphic between individuals. Nowadays, genotyping of STR marker is widely known and used for the genetic identification of individuals in forensic ...
Mohammadreza Nassiri +6 more
doaj
Structural Protein-based Flexible Whispering Gallery Mode Resonators [PDF]
arXiv, 2016 Nature provides a set of solutions for photonic structures that are finely tuned, organically diverse and optically efficient. Exquisite knowledge of structure-property relationships in proteins aids in the design of materials with desired properties for building devices with novel functionalities, which are difficult to achieve or previously ...
arxiv
FEBS Open Bio, EarlyView.Etoposide, a topoisomerase II inhibitor, reduces O‐GlcNAcylation in HepG2 liver cancer cells. Further inhibition of O‐GlcNAc transferase by OSMI‐1 enhanced etoposide‐induced apoptosis, lowering the IC50 for viability and increasing the EC50 for cytotoxicity.
Jaehoon Lee +5 more
wiley +1 more source
Reconstitution of intramembrane particles in recombinants of erythrocyte protein band 3 and lipid: effects of spectrin-actin association. [PDF]
, 1976 John Yu, Daniel Branton
openalex +1 more source
Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae
Biotechnology and Bioengineering, 2012 Yeast Saccharomyces cerevisiae has become an attractive cell factory for production of commodity and speciality chemicals and proteins, such as industrial enzymes and pharmaceutical proteins.
Zihe Liu +4 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu +6 more
wiley +1 more source