Results 161 to 170 of about 1,103,976 (304)

Plant‐Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors

Advanced Healthcare Materials, EarlyView.
Potato virus X is a filamentous RNA plant virus that can be engineered into a molecular tool for cancer therapy. We produced genetically‐encoded virus‐derived nanoparticles decorated with nanobodies targeting cancer cell receptors, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2).
Enrique Lozano‐Sanchez, Miguel A. Moreno‐Gonzalez, Fernando Merwaiss, Nicole F. Steinmetz, José‐Antonio Daròs   +4 more
wiley   +1 more source

A katE katG double-knockout E. coli strain eliminates the risk of catalase contamination in recombinant proteins. [PDF]

Appl Microbiol Biotechnol
Scholz AT, Coppo L, Nolan E, Hernandez M, Wang X, Mishra P, Schnell R, Pató ZA, Chen Y, Dagnell M, Andor A, Cheng Q, Arnér ESJ.   +12 more
europepmc   +1 more source

A Physiological Microfluidic Blood–Brain‐Barrier Model for In Vitro Study of Nanoparticle Trafficking and Accumulation

Advanced Healthcare Materials, EarlyView.
A human microfluidic blood‐brain barrier (mBBB) model enables spatially resolved comparison of nanoparticle trafficking. Extracellular vesicles (EVs), liposomes, and nanoplastics exhibit distinct transport and disruption behaviors, revealing that membrane composition and uptake pathways govern BBB interaction.
Bryan B. Nguyen, Neona M. Lowe, Sophia Kellogg, Kuan‐Wei Huang, Hannah O'Toole, Elizabeth J. Hale, Venktesh S. Shirure, Bhupinder S. Shergill, Steven C. George, Randy P. Carney   +9 more
wiley   +1 more source

A Versatile Plasmid System for Translational Control and Secretion of Recombinant Proteins in Mycobacteria. [PDF]

ACS Synth Biol
Gigante Pereira V, Rezende Corrêa P, Barros RM, Corrêa MBC, Dellagostin OA, Mendonça-Lima L, Schwarz MGA.   +6 more
europepmc   +1 more source

3D Soft Hydrogels Induce Human Mesenchymal Stem Cells “Deep” Quiescence

Advanced Healthcare Materials, EarlyView.
Three‐dimensional soft hydrogels mimicking the bone marrow niche induce deep quiescence in human mesenchymal stem cells. Unlike 2D culture, 3D matrices halt proliferation, regulate cell‐cycle and quiescence markers, and downregulate mTORC1 signaling, preserving stem cell phenotype and therapeutic potential ex vivo.
David Boaventura Gomes, Timo Rademakers, Ana Filipa Henriques Lourenço, Andrea Calore, Clarissa Tomasina, Denis van Beurden, Jinjie Venema, Bryan Chömpff, Hong Liu, Nicole Bouvy, Sandra Camarero‐Espinosa, Lorenzo Moroni   +11 more
wiley   +1 more source

Application of a novel fusion tag system for enhanced soluble expression of recombinant proteins in Escherichia coli. [PDF]

BMC Biotechnol
Luo LZ, Cai JT, Tan ZY, Chen YQ, Hu Z, Wang HH, Zhang WB, Ma JC.   +7 more
europepmc   +1 more source

Recapitulating Endochondral Ossification for Bone Repair: From Development to Engineering Strategy

Advanced Healthcare Materials, EarlyView.
This review summarizes the developmental basis of endochondral ossification (ECO) and its applications in bone tissue engineering (BTE). It first outlines the key biological processes and signaling pathways underlying ECO, then discusses biomaterial‐based engineering strategies derived from these principles, and finally highlights future directions for
Yiqi Su, Zihao He, Qianqian Chen, Long Chen, Caiyi Wei, Zijin Zhou, Jianhao Lin, Hui Li, Dan Xing   +8 more
wiley   +1 more source

On-Column Dual-Gradient Refolding for Efficient Recovery of Insoluble Affinity-Tagged Recombinant Proteins. [PDF]

Bio Protoc
Vlaskina A, Petrenko D, Agapova Y, Kuzminkova A, Evteeva M, Patrushev M.   +5 more
europepmc   +1 more source

Engineered Protein‐Based Ionic Conductors for Sustainable Energy Storage Applications

Advanced Materials, EarlyView.
Rational incorporation of charged residues into an engineered, self‐assembling protein scaffold yields solid‐state protein films with outstanding ionic conductivity. Salt‐doping further enhances conductivity, an effect amplified in the engineered variants. These properties enable the material integration into an efficient supercapacitor.
Juan David Cortés‐Ossa, Paolo Blesio, Marcial Fernandez‐Castro, Lisa Almonte, Maxence Fernandez, Mantas Liutkus, Perumal Pandurangan, Carlos Sabater, Aitor Villaverde, Manuel Melle‐Franco, Nurit Ashkenasy, Felipe Jiménez‐Ángeles, M. Carmen Morant‐Miñana, M. Reyes Calvo, Aitziber L. Cortajarena   +14 more
wiley   +1 more source

Protein-Derived Signal Peptides Induced by Agrobacterium Infection Promote the Secretion of Recombinant Proteins in Nicotiana benthamiana. [PDF]

Plant Biotechnol J
Kajiura H, Yamamoto K, Misaki R, Fujiyama K.   +3 more
europepmc   +1 more source