Results 171 to 180 of about 714,113 (219)
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Selenomethionine Labeling of Recombinant Proteins
2007Selenomethionine incorporation is a standard method for determining the phases in protein crystallography by single- or multiwavelength anomalous dispersion. Recombinant expression of selenomethionine-containing protein in non-auxotrophic Pichia pastoris strains yield an incorporation of about 50%.
Anna M. Larsson, T. Alwyn Jones
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Recombinant protein production and streptomycetes
Journal of Biotechnology, 2012The biopharmaceutical market has come a long way since 1982, when the first biopharmaceutical product, recombinant human insulin, was launched. Just over 200 biopharma products have already gained approval. The global market for biopharmaceuticals which is currently valued at over US$99 billion has been growing at an impressive compound annual growth ...
Lieve Van Mellaert +4 more
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Recombinant Protein Production in Yeasts
2004Recombinant DNA (rDNA) technologies (genetic, protein, and metabolic engineering) allow the production of a wide range of peptides, proteins, and biochemicals from naturally nonproducing cells. This technology, now approx 25 yr old, is becoming one of the most important technologies developed in the 20th century.
Porro, D, Mattanovich, D
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Purification of BmR1 Recombinant Protein
The Protein Journal, 2010This paper describes a refinement in the purification step that facilitated the downstream recovery of high purity BmR1 recombinant protein, which is a protein used as a test reagent in the commercialized rapid tests for detection of lymphac filariasis i.e. Brugia Rapid™ and panLF rapid™.
Ahmad R. M. Yahya +4 more
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Recombinant proteins for genetic disease
Clinical Genetics, 1999The era of molecular biology has led to the development of powerful tools capable of generating therapeutics for genetic disorders. Although there is much current emphasis placed on the development of ‘gene therapy’ for human disease, developments in the production and availability of recombinant proteins are likely to have a more substantial impact on
Christopher S. Russell, Lorne A. Clarke
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Preparation of Recombinant Proteins in Milk
2004Using transgenic animals as the source of recombinant proteins has several specific advantages. Large amounts of proteins can be obtained, essentially from milk. These proteins are often properly processed. They are in a number of cases correctly folded, assembled, cleaved, glycosylated, gamma-carboxylated, and so on.
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2016
Proteomics has opened the door to identify more and more clinically relevant proteins. Once identified, these proteins need to be studied in detail, including expression of the protein in model organisms by using recombinant DNA techniques. Some proteins will become therapeutic agents, and large amounts of purified protein will be required.
David P. Clark, Nanette J. Pazdernik
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Proteomics has opened the door to identify more and more clinically relevant proteins. Once identified, these proteins need to be studied in detail, including expression of the protein in model organisms by using recombinant DNA techniques. Some proteins will become therapeutic agents, and large amounts of purified protein will be required.
David P. Clark, Nanette J. Pazdernik
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Recombinant Proteins in Vaccine Development
2003The outer membrane of Neisseria meningitidis contains a variety of proteins with the potential for inclusion in new meningococcal vaccines (1). Studies on the vaccine potential of these proteins would be facilitated by the production of pure recombinant protein, free from other components of the Neisseria outer membrane.
John E. Heckels +2 more
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Overproduction of recombinant proteins in plants
Plant Science, 2012Recombinant protein production in microbial hosts and animal cell cultures has revolutionized the pharmaceutical and industrial enzyme industries. Plants as alternative hosts for the production of recombinant proteins are being actively pursued, taking advantage of their unique characteristics.
Erin Egelkrout, Vidya Rajan, John Howard
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Recombinant Protein Expression in Nicotiana
2010Recombinant protein pharmaceuticals are now widely used in treatment of chronic diseases, and several recombinant protein subunit vaccines are approved for human and veterinary use. With growing demand for complex protein pharmaceuticals, such as monoclonal antibodies, manufacturing capacity is becoming limited.
Keith R. Davis +2 more
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