Results 181 to 190 of about 714,113 (219)

Capillary electrophoresis of recombinant proteins

Journal of Chromatography B: Biomedical Sciences and Applications, 1997
Many naturally occurring proteins which are used therapeutically have been cloned and expressed in large quantities in bacterial, yeast or mammalian systems. Purification of these proteins by column chromatography generates high purity products with low levels of host protein contaminants.
Stephen A. Tate, Katharine A. Denton
openaire   +3 more sources

Recombinant proteins in the diagnosis of toxoplasmosis

APMIS, 2010
Kotresha D, Rahmah N. Recombinant proteins in the diagnosis of toxoplasmosis. APMIS 2010; 118: 529–42. Toxoplasma gondii is an important human pathogen with a worldwide distribution. It is primarily of medical importance for pregnant women and immunocompromised patients.
D. Kotresha, Rahmah Noordin
openaire   +3 more sources

Recombinant Therapeutic Protein Vaccines

Protein & Peptide Letters, 2013
Recombinant technology has ushered in a new era for the pharmaceutical industry. Protein therapeutics, including plasma-derived products and antibodies obtained from the serum of infected patients, have been successfully adopted and utilized to treat various indications.
Satoshi Ohtake, Tsutomu Arakawa
openaire   +3 more sources

The expression of recombinant proteins in yeasts

Current Opinion in Biotechnology, 1996
The methylotrophic yeasts Hansenula polymorpha and Pichia pastoris are rapidly becoming the systems of choice for the expression of recombinant proteins in yeast. However, the powerful genetic techniques available in Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe are still exploited to establish models to study medically ...
openaire   +3 more sources

SCHEMA-Guided Protein Recombination

2004
Publisher Summary This chapter examines the different aspects of SCHEMA-guided protein recombination. SCHEMA is a scoring function that predicts which elements in homologous proteins can be swapped without disturbing the integrity of the structure. Using the structural coordinates of the parent proteins, SCHEMA identifies pairs of residues that are ...
Silberg, Jonathan J., Endelman, Jeffrey B., Arnold, Frances H.   +2 more
openaire   +4 more sources

Heavy Labeling of Recombinant Proteins

2007
Because of the cost of isotopic chemicals and heterologous proteins to produce, an economical 15N/13C isotopic labeling method is critically needed. Four protocols have been tested for the expression of Ovine interferon-tau in Pichia pastoris. 13C-glucose in place of 13C-glycerol as well as the need for 15N/13C-sources were evaluated during the growth ...
openaire   +3 more sources

A Universal Tag for Recombinant Proteins

Archives of Biochemistry and Biophysics, 1996
Incorporation of tags into recombinant proteins can facilitate their identification and purification. In addition, these tags can also be used to monitor the trafficking or localization of the recombinant proteins inside the cells. Several such tags have been developed. However, the lengths of these tags make it cumber-some to incorporate them into the
Jane M. Li, T. Chyau Liang, Sue Hwa Lin, Jer Tsong Hsieh, Weiping Luo   +4 more
openaire   +3 more sources

Challenges in production of recombinant proteins

Bioprocess and Biosystems Engineering, 2007
The sequencing of the human genome and genomes from lower species has led molecular biology research into the era of proteomics. Starting from the cDNA’s, a large number of proteins have to be expressed in quantities large enough to allow determining of their structure and function, which forms the basis for the development of new pharmaceutical ...
openaire   +3 more sources

Industrial Challenges of Recombinant Proteins

2019
The use of recombinant DNA methods to produce large quantities of protein for therapeutic uses has revolutionized medicine. Industrial challenges for manufacture of biotherapeutic proteins are related to the characteristics of these proteins and the increasing quantities required to address needs of patients, worldwide. A brief overview of therapies in
Michael R. Ladisch, Scott R. Rudge
openaire   +2 more sources

Characterization of Recombinant Proteins

Current Protocols in Protein Science, 2003
Roger H. Pain, Paul T. Wingfield
openaire   +2 more sources