Results 111 to 120 of about 356,079 (353)

Recombineering: A Homologous Recombination-Based Method of Genetic Engineering

Nature Protocols, 2009
Recombineering is an efficient method of in vivo genetic engineering applicable to chromosomal as well as episomal replicons in Escherichia coli. This method circumvents the need for most standard in vitro cloning techniques.
S. K. Sharan, L. Thomason, S. Kuznetsov, D. Court   +3 more
semanticscholar   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Evidence for RNA recombination between distinct isolates of Pepino mosaic virus.

, 2010
Genetic recombination plays an important role in the evolution of virus genomes. In this study we analyzed publicly available genomic sequences of Pepino mosaic virus (PepMV) for recombination events using several bioinformatics tools.
Leunissen, Jack, Kuzniar, A., Hasiów-Jaroszewska, B., Peters, S.A., Peters, Sander, Leunissen, J.A.M., Pospieszny, Henryk, Hasiów-Jaroszewska, Beata, Kuzniar, Arnold, Pospieszny, H.   +9 more
core  

Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination

, 2008
Over a period of a few years, Pepino mosaic virus (PepMV) has become one of the most important viral diseases in tomato production worldwide. Infection by PepMV can cause a broad range of symptoms on tomato plants, often leading to significant financial ...
Wittemans, Lieve, Paeleman, A., Thomma, B.P.H.J., Paeleman, Anneleen, Vanachter, Alfons C. R. C., Goen, K., Hanssen, Inge M., Lievens, Bart, Hanssen, I.M., Bragard, C., Wittemans, L.P.F., Vanachter, A.C.R.C., Vanachter, Alfons C.R.C., Thomma, Bart P.H.J., Bragard, Claude, Groen, Kris, Goen, Kris, Thomma, Bart P. H. J., Lievens, B.   +18 more
core   +1 more source

Bayesian modeling of recombination events in bacterial populations [PDF]

, 2008
Background: We consider the discovery of recombinant segments jointly with their origins within multilocus DNA sequences from bacteria representing heterogeneous populations of fairly closely related species.
Marttinen, P., Hanage William P, Mahenthiralingam Eshwar, Adam Baldwin, Jukka Corander, Marttinen Pekka, Hanage, William P., Pekka Marttinen, Hanage, W. P., Marttinen, Pekka, Dowson, Christopher G., Baldwin, A., Baldwin Adam, Dowson, C., Chris Dowson, Corander, J., Baldwin, Adam, Dowson Chris, William P Hanage, Eshwar Mahenthiralingam, Corander Jukka, Mahenthiralingam, Eshwar   +21 more
core   +1 more source

Homologous recombination deficiency: Exploiting the fundamental vulnerability of ovarian cancer

Cancer Discovery, 2015
Approximately 50% of epithelial ovarian cancers (EOCs) exhibit defective DNA repair via homologous recombination (HR) due to genetic and epigenetic alterations of HR pathway genes.
P. Konstantinopoulos, Raphael Ceccaldi, G. Shapiro, A. D’Andrea   +3 more
semanticscholar   +1 more source

Aging Is a Key Driver for Adult Acute Myeloid Leukemia

Aging and Cancer, EarlyView.
Acute myeloid leukemia (AML) is a classical age‐related hematologic malignancy, and a key driver of AML is aging, which profoundly regulates intrinsic factors such as genomic instability, epigenetic reprogramming, and metabolic dysregulation, and alters bone marrow microenvironment.
Rong Yin, Haojian Zhang
wiley   +1 more source

Determinants of selectivity in Xer site-specific recombination

, 1996
A remarkable property of some DNA-binding proteins that can interact with and pair distant DNA segments is that they mediate their biological function only when their binding sites are arranged in a specific configuration. Xer site-specific recombination
Blakely, G, Sherratt, D
core   +1 more source

Fungal clones win the battle, but recombination wins the war

IMA Fungus, 2019
Clonal reproduction is common in fungi and fungal-like organisms during epidemics and invasion events. The success of clonal fungi shaped systems for their classification and some pathogens are tacitly treated as asexual.
André Drenth, Alistair R. McTaggart, Brenda D. Wingfield   +2 more
doaj   +1 more source

Optimization of the Production of Rubber Compounds Using Mathematical Models

Advanced Engineering Materials, EarlyView.
Rubber compounds were mixed in a batch internal mixer, and symbolic regression was used to derive mathematical models linking recipe and process parameters to ram path, torque, and mixing quality (incorporation, dispersion, distribution). Subsequent optimization with evolutionary algorithms identified operating conditions that reduce specific energy ...
Anke Bardehle, Andreas Limper, Ulrich Giese, Carlos Lucke, Mario Beiner, Christoph Gögelein, Lisa Leuchtenberger‐Engel, Christian Hopmann   +7 more
wiley   +1 more source