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Genetic Engineering by DNA Recombineering
Current Protocols in Chemical Biology, 2019 AbstractRecombineering inserts PCR products into DNA using homologous recombination. A pair of short homology arms (50 base pairs) on the ends of a PCR cassette target the cassette to its intended location. These homology arms can be easily introduced as 5′ primer overhangs during the PCR reaction.
Papa, Louis John, Shoulders, Matthew D.
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Genetic recombination in Nocardia asteroides [PDF]
Journal of Bacteriology, 1982 Gene recombination between strains of Nocardia asteroides of diverse origins has been demonstrated. In particular pairwise combinations, recombinants made up 0.01% of the population. All nine selectable recombinant classes were recovered from a cross KK4-47 his-10 leu-1 and KK6-119 met-3 phe-3.
M L Little, K L Kasweck
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Random-sequence genetic oligomer pools display an innate potential for ligation and recombination
eLife, 2018 Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes. Here, we describe an innate
Hannes Mutschler +7 more
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Making serine integrases work for us [PDF]
, 2017 DNA site-specific recombinases are enzymes (often associated with mobile DNA elements) that catalyse breaking and rejoining of DNA strands at specific points, thereby bringing about precise genetic rearrangements.
Stark, W. Marshall
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Identification and manipulation of the molecular determinants influencing poliovirus recombination.
PLoS Pathogens, 2013 The control and prevention of communicable disease is directly impacted by the genetic mutability of the underlying etiological agents. In the case of RNA viruses, genetic recombination may impact public health by facilitating the generation of new viral
Charles Runckel +3 more
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mSphere, 2020 Illness caused by the pathogen Clostridioides difficile is widespread and can range in severity from mild diarrhea to sepsis and death. Strains of C. difficile isolated from human infections exhibit great genetic diversity, leading to the hypothesis that
Hannah D. Steinberg, Evan S. Snitkin
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The influence of recombination on human genetic diversity. [PDF]
PLoS Genetics, 2006 In humans, the rate of recombination, as measured on the megabase scale, is positively associated with the level of genetic variation, as measured at the genic scale.
Chris C A Spencer +8 more
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Evidence for RNA recombination between distinct isolates of Pepino mosaic virus. [PDF]
, 2010 Genetic recombination plays an important role in the evolution of virus genomes. In this study we analyzed publicly available genomic sequences of Pepino mosaic virus (PepMV) for recombination events using several bioinformatics tools.
Hasiów-Jaroszewska, B. +4 more
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Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia
FEBS Letters, EarlyView.This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley +1 more source
Genetic analysis of variation in human meiotic recombination.
PLoS Genetics, 2009 The number of recombination events per meiosis varies extensively among individuals. This recombination phenotype differs between female and male, and also among individuals of each gender.
Reshmi Chowdhury +4 more
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