In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes +32 more
wiley +1 more source
miR-146a/b-5p-IRAK1-NF-κB Axis Regulates Unexplained Recurrent Spontaneous Abortion. [PDF]
He F +7 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Clinical manifestations of injured tracheal and esophageal branches of the recurrent laryngeal nerve following thyroid surgery. [PDF]
Lv F +7 more
europepmc +1 more source
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Endoscopic ultrasound-guided gallbladder drainage and electrohydraulic lithotripsy for the treatment of gallbladder neck stones. [PDF]
Wang Y, Dong Q, Li L, Liu X, Li W, Li Q.
europepmc +1 more source
Transcriptome combined with single-cell data to construct a prognostic model for glycosylation-related genes in osteosarcoma. [PDF]
Ding N +5 more
europepmc +1 more source
Ivonescimab combined with chemotherapy for the treatment of metastatic cervical cancer: A case report and literature review. [PDF]
Xu M, Qiu S, Zhou J, Zhang Y.
europepmc +1 more source

