Results 131 to 140 of about 814 (162)
Bacillus subtilis RecA with DprA–SsbA antagonizes RecX function during natural transformation [PDF]
Nucleic Acids Research, 2017 Bacillus subtilis DprA and RecX proteins, which interact with RecA, are crucial for efficient chromosomal and plasmid transformation. We showed that RecA, in the rATP·Mg2+ bound form (RecA·ATP), could not compete with RecX, SsbA or SsbB for assembly onto single-stranded (ss)DNA, but RecA·dATP partially displaced these proteins from ssDNA. RecX promoted
Shimin Le +2 more
exaly +6 more sources
FEBS Letters, 2014 Using molecular modeling techniques we have built the full atomic structure and performed molecular dynamics simulations for the complexes formed by Escherichia coli RecX protein with a single-stranded oligonucleotide and with RecA presynaptic filament ...
Alexey V Shvetsov +2 more
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A RecA Filament Capping Mechanism for RecX Protein [PDF]
Molecular Cell, 2004 The RecX protein is a potent inhibitor of RecA protein activities. RecX functions by specifically blocking the extension of RecA filaments. In vitro, this leads to a net disassembly of RecA protein from circular single-stranded DNA. Based on multiple observations, we propose that RecX has a RecA filament capping activity.
Shelley L Lusetti +2 more
exaly +3 more sources
SSB Antagonizes RecX-RecA Interaction [PDF]
Journal of Biological Chemistry, 2008 The RecX protein of Escherichia coli inhibits the extension of RecA protein filaments on DNA, presumably by binding to and blocking the growing filament end. The direct binding of RecX protein to single-stranded DNA is weak, and previous reports suggested that direct binding to DNA did not explain the effects of RecX.
Dmitry Baitin, Michael M Cox
exaly +3 more sources
The RecF Protein Antagonizes RecX Function via Direct Interaction [PDF]
Molecular Cell, 2006 The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX. In vitro, efficient RecA filament formation onto single-stranded DNA binding protein (SSB)-coated circular single-stranded DNA (ssDNA) in the presence ...
Shelley L Lusetti +2 more
exaly +3 more sources
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Molecular modeling of RecX reveals its mode of interaction with RecA
Biochemical and Biophysical Research Communications, 2003The protein RecA is involved in homologous recombination, DNA repair and also catalyzes DNA strand exchange. RecX gene is downstream of recA and the gene product RecX is supposed to be important for RecA regulation. Recombinant RecX is purified to homogeneity, and circular dichroism (CD) and FTIR spectroscopy show the protein to exist mostly in helical
Joykrishna Dey, Amit Kumar Das
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Less Is More: Neisseria gonorrhoeae RecX Protein Stimulates Recombination by Inhibiting RecA [PDF]
Journal of Biological Chemistry, 2010 Escherichia coli RecX (RecX(Ec)) is a negative regulator of RecA activities both in the bacterial cell and in vitro. In contrast, the Neisseria gonorrhoeae RecX protein (RecX(Ng)) enhances all RecA-related processes in N. gonorrhoeae. Surprisingly, the RecX(Ng) protein is not a RecA protein activator in vitro.
Elizabeth A Stohl +2 more
exaly +3 more sources
Expression, purification, and DNA-binding activity of the Herbaspirillum seropedicae RecX protein
Protein Expression and Purification, 2004The Herbaspirillum seropedicae RecX protein participates in the SOS response: a process in which the RecA protein plays a central role. The RecX protein of the H. seropedicae, fused to a His-tag sequence (RecX His-tagged), was over-expressed in Escherichia coli and purified by metal-affinity chromatography to yield a highly purified and active protein.
Carolina Weigert Galvao +2 more
exaly +3 more sources
Inhibition of RecA Protein by the Escherichia coli RecX Protein [PDF]
Journal of Biological Chemistry, 2004 Shelley L Lusetti, Michael M Cox
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Evolutionary pathways of an ancient gene recX
Gene, 2007RecX is a regulator of RecA activity by interacting with RecA protein or RecA filaments. Genes encoding RecX were found in genomes of a wide diversity of bacteria and some plants (e.g., Arabidopsis thaliana and Oryza sativa). Our comparative genome analysis showed that although members of the RecX family are found in many bacterial species, they are ...
Jun, Lin +3 more
openaire +2 more sources