Results 141 to 150 of about 814 (162)

Deinococcus radiodurans RecX and Escherichia coli RecX proteins are capable to replace each other in vivo and in vitro

Russian Journal of Genetics, 2016
A plasmid carrying the Deinococcus radiodurans recXgene under the control of a lactose promoter decreases the Escherichia coli cell resistance to UV irradiation and γ irradiation and also influences the conjugational recombination process. The D. radiodurans. RecX protein functions in the Escherichia coli cells similarly to the E.
I V, Bakhlanova, D M, Baitin
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Escherichia coli RecX Inhibits RecA Recombinase and Coprotease Activities in Vitro and in Vivo [PDF]

Journal of Biological Chemistry, 2003
In Escherichia coli the RecA protein plays a pivotal role in homologous recombination, DNA repair, and SOS repair and mutagenesis. A gene designated recX (or oraA) is present directly downstream of recA in E. coli; however, the function of RecX is unknown. In this work we demonstrated interaction of RecX and RecA in a yeast two-hybrid assay.
Elizabeth A Stohl, Katsumi Morimatsu, Stephen C Kowalczykowski   +2 more
exaly   +3 more sources

Purification and characterization of the Thermus thermophilus HB8 RecX protein

Protein Expression and Purification, 2007
The RecA protein plays a central role in homologous recombination by promoting strand exchange between ssDNA and homologous dsDNA. Since RecA alone can advance this reaction in vitro, it is widely used in gene manipulation techniques. The RecX protein downregulates the function of RecA, indicating that it could be used as an inhibitor to control the ...
Koutaro, Jimbo, Jin, Inoue, Tokiha, Masuda, Takehiko, Shibata, Tsutomu, Mikawa   +4 more
openaire   +2 more sources

The extended N-terminus of Mycobacterium smegmatis RecX potentiates its ability to antagonize RecA functions

Biochimica Et Biophysica Acta - Proteins and Proteomics, 2020
The members of the RecX family of proteins have a unique capacity to regulate the catalytic activities of RecA/Rad51 proteins in both prokaryotic and eukaryotic organisms. However, our understanding of the functional roles of RecX in pathogenic and non-pathogenic mycobacteria has been limited by insufficient knowledge of the molecular mechanisms of its
K Muniyappa
exaly   +3 more sources

Molecular Characterization and Expression of the recX Gene of Xanthomonas campestris pv. citri

Current Microbiology, 2001
Two genes important in DNA repair, recA and lexA, were recently identified in Xanthomonas campestris pathovar citri (X.c. pv. citri). An open reading frame located immediately downstream of lexA and recA has now been isolated from this pathovar and characterized. This 486-bp open reading frame encodes a protein of 162 amino acids and shares substantial
M K, Yang, M E, Chou, Y C, Yang
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The recX gene potentiates homologous recombination in Neisseria gonorrhoeae

Molecular Microbiology, 2001
In the pathogen Neisseria gonorrhoeae (Gc), the RecA protein is necessary for DNA repair, DNA transformation and pilus antigenic variation. Many bacteria contain a gene, recX, which has been suggested to downregulate recA through an unknown mechanism. To investigate the possible role of recX in Gc, we cloned and insertionally inactivated the recX gene.
E A, Stohl, H S, Seifert
openaire   +2 more sources

TherecXgene product is involved in the SOS response inHerbaspirillum seropedicae

Canadian Journal of Microbiology, 2003
The recA and the recX genes of Herbaspirillum seropedicae were sequenced. The recX is located 359 bp downstream from recA. Sequence analysis indicated the presence of a putative operator site overlapping a probable σ70-dependent promoter upstream of recA and a transcription terminator downstream from recX, with no apparent promoter sequence in the ...
Carolina W, Galvão, Fábio O, Pedrosa, Emanuel M, Souza, M Geoffrey, Yates, Leda S, Chubatsu, Maria Berenice R, Steffens   +5 more
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recX, a new SOS gene that is co-transcribed with the recA gene in Escherichia coli

DNA Repair, 2003
recX is a small open reading frame located downstream of recA that is conserved in many bacteria. In Escherichia coli, the recX gene (also named oraA) is a 501 bp open reading frame that encodes a predicted basic protein. Transcriptional analysis by Northern blots showed that in E. coli the recX gene is SOS-regulated.
Vincent, Pagès, Nicole, Koffel-Schwartz, Robert P P, Fuchs   +2 more
openaire   +2 more sources

RecX is Involved In the Switch between DNA Damage Response and Normal Metabolism in D. radiodurans

Journal of Biochemistry, 2009
Apart from inhibiting RecA activity through protein-protein interactions, Deinococcus radiodurans RecX inhibits the expression of RecA and two other anti-oxidant proteins. To identify the repertoire of proteins regulated by RecX, comparative proteomic studies were undertaken on a wild-type strain (R1) and recX null mutant (RecX(-)).
Duohong, Sheng, Jiandong, Jao, Mingfeng, Li, Peng, Xu, Junbing, Zhang   +4 more
exaly   +3 more sources

Complexes of RecA with LexA and RecX Differentiate Between Active and Inactive RecA Nucleoprotein Filaments

Journal of Molecular Biology, 2003
The bacterial RecA protein has been the dominant model system for understanding homologous genetic recombination. Although a crystal structure of RecA was solved ten years ago, we still do not have a detailed understanding of how the helical filament formed by RecA on DNA catalyzes the recognition of homology and the exchange of strands between two DNA
Margaret S, VanLoock, Xiong, Yu, Shixin, Yang, Vitold E, Galkin, Hao, Huang, Shyamala S, Rajan, Wayne F, Anderson, Elizabeth A, Stohl, H Steven, Seifert, Edward H, Egelman   +9 more
openaire   +2 more sources