Results 51 to 60 of about 4,116,135 (338)

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Dynamics of neutrophil phenotype and function in sickle cell disease

Frontiers in Immunology
IntroductionWhile sickle cell disease (SCD) is primarily acknowledged as an erythrocyte disorder, emerging evidence suggests a role for altered neutrophil phenotype and function in SCD pathophysiology and disease severity.
Aafke E. Gaartman, Aafke E. Gaartman, Lydian A. de Ligt, Lydian A. de Ligt, Lydian A. de Ligt, Boukje M. Beuger, Anton T. J. Tool, Martijn Veldthuis, Taco W. Kuijpers, Taco W. Kuijpers, Rob van Zwieten, Bart J. Biemond, Robin van Bruggen, Erfan Nur, Erfan Nur   +14 more
doaj   +1 more source

Proteomic analysis of plasma from children with sickle cell anemia and silent cerebral infarction

Haematologica, 2018
Silent cerebral infarction is the most common neurological abnormality in children with sickle cell anemia, affecting 30-40% of 14 year olds. There are no known biomarkers to identify children with silent cerebral infarcts, and the pathological basis is ...
Sanjay Tewari, George Renney, John Brewin, Kate Gardner, Fenella Kirkham, Baba Inusa, James E Barrett, Stephan Menzel, Swee Lay Thein, Malcolm Ward, David C. Rees   +10 more
doaj   +1 more source

Rare-cell enrichment by a rapid, label-free, ultrasonic isopycnic technique for medical diagnostics [PDF]

, 2014
One significant challenge in medical diagnostics lies in the development of label-free methods to separate different cells within complex biological samples.
Bell, Bourquin, Chappuis, Franke, Friend, Grover, Kumar, Leif, Lejon, Minion, Nash, Raghavan, Reboud, Rogers, Tan, Urdea, Wilson, Yager, Yatsushiro   +18 more
core   +1 more source

Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation

FEBS Letters, EarlyView.
The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt, Marcus S. Münchhoff, Jacqueline Krohn, Philip M. Palarz, Manuel H. Taft, Johannes N. Greve, Nataliya Di Donato, Falk F. R. Buettner, Dietmar J. Manstein   +8 more
wiley   +1 more source

Persistent accumulation of unrepaired DNA damage in rat cortical neurons: nuclear organization and ChIP-seq analysis of damaged DNA

Acta Neuropathologica Communications, 2018
Neurons are highly vulnerable to DNA damage induced by genotoxic agents such as topoisomerase activity, oxidative stress, ionizing radiation (IR) and chemotherapeutic drugs.
Jorge Mata-Garrido, Olga Tapia, Iñigo Casafont, Maria T. Berciano, Ana Cuadrado, Miguel Lafarga   +5 more
doaj   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

The Red Cell [PDF]

BMJ, 1972
  +12 more sources

Automated and closed clinical-grade manufacturing protocol produces potent NK cells against neuroblastoma cells and AML blasts

Scientific Reports
Natural killer (NK) cells are a promising allogeneic immunotherapy option due to their natural ability to kill tumor cells, and due to their apparent safety.
Farhana Jahan, Leena Penna, Annu Luostarinen, Laurens Veltman, Heidi Hongisto, Kaarina Lähteenmäki, Sabine Müller, Seppo Ylä-Herttuala, Matti Korhonen, Kim Vettenranta, Anita Laitinen, Urpu Salmenniemi, Erja Kerkelä   +12 more
doaj   +1 more source

BET protein inhibition regulates cytokine production and promotes neuroprotection after spinal cord injury

Journal of Neuroinflammation, 2019
Background Spinal cord injury (SCI) usually causes a devastating lifelong disability for patients. After a traumatic lesion, disruption of the blood-spinal cord barrier induces the infiltration of macrophages into the lesion site and the activation of ...
Judith Sánchez-Ventura, Jesús Amo-Aparicio, Xavier Navarro, Clara Penas   +3 more
doaj   +1 more source