Results 191 to 200 of about 5,500,427 (306)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Chromosome-level reference genome of a foundational California native legume, Acmispon strigosus. [PDF]

J Hered
Torres Martínez L, Escalona M, Toffelmier E, Miller C, Sahasrahbudhe R, Marimuthu MPA, Nguyen O, Chumchim N, Beraut E, Sacco S, Seligmann W, Fairbairn CW, Cooper RD, Shaffer HB, Purcell J, Sachs JL.   +15 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

A high-quality reference genome for the ornate shrew (Sorex ornatus). [PDF]

J Hered
Linn IJ, Escalona M, Nguyen O, Marimuthu MPA, Chumchim N, Fairbairn CW, Seligmann W, Beraut E, Conroy CJ, Bowie RCK, Nachman MW.   +10 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Reference genome of the leopard seal (Hydrurga leptonyx), a Southern Ocean apex predator

Frontiers in Genetics
J. Canitz, S. S. Kienle, S. S. Kienle, K. van der Linde, R. Borras-Chavez, R. Borras-Chavez, E. S. Sperou, A. Leahy, A. Leahy, S. Rivera, S. Rivera, M. Autenrieth, J. I. Hoffman, J. I. Hoffman, J. I. Hoffman, J. I. Hoffman, J. I. Hoffman, C. A. Bonin   +17 more
doaj   +1 more source

A telomere-to-telomere reference genome assembly of the Hypomesus nipponensis. [PDF]

Sci Data
Zhou Y, Fang D, You Y, Tang F, Bai Y, Zhang M, Li X, Deng G, Xu D.   +8 more
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Reference Genome Assembly of a Tetraploid Accession of the Tuber Crop Tropaeolum tuberosum. [PDF]

Genome Biol Evol
Ruiz-Mateus D, Scheffler I, Márquez-Cardona MDP, Greb T, Terán W, Hunziker P.   +5 more
europepmc   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source