Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
KNexPHENIX: A PHENIX-Based Workflow for Improving Cryo-EM and Crystallographic Structural Models. [PDF]
J Chem Inf ModelNandi S, Conn GL.
europepmc +1 more source
Refinement dell'Analgesia e dell'Anestesia negli Animali da Laboratorio
, 2009 The intrinsic value of each laboratory animal requires skilled refinement procedures to minimize suffering, implementing fine sedation, analgesia and anaesthesia standards.The remarkable progress in anaesthetic techniques achieved after Directive 86/609 ...
VESCE, GIOVANNI
core
FEBS Open Bio, EarlyView.UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Structure-Preserving Point Cloud Completion with Symmetry-Guided Progressive Refinement. [PDF]
Sensors (Basel)Zhao S, Niu Y.
europepmc +1 more source
FEBS Open Bio, EarlyView.The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley +1 more source
Sensory Blurring in Nociplastic Pain: The Role of Descending Inhibitory Dysfunction and Gut-Brain Axis Alterations in Older Adults. [PDF]
Geriatrics (Basel)Nagamine T.
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Mechanistic origin of high-cycle fatigue enhancement by grain refinement in AZ81 magnesium alloy for sports equipment. [PDF]
PLoS OneLi D, He L.
europepmc +1 more source
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source